Ulrich Andreas Dietz

Avaliação da ação da Aroeira (Schinus terebinthifolius Raddi) na cicatrização de feridas cirúrgicas em bexiga de ratos

PURPOSE: To evaluate the hydroalcoholic aroeira extract in the healing process of surgical incisions in the bladder of rats in an experimental comparative study. METHODS: Forty male Wistar rats were used. All of them underwent a median abdominal incision, with an 1cm cistotomy, followed by one plan suture with separated stitches of poliglactine 910 5-0. The animals were then divided into two groups of 20 rats each, and named the aroeira group (GA) and the control group (GC). In the first one, 80% hydro-alcoholic plant extract was injected in the peritoneal cavity, in a one dose of 100mg/kg. The second group, a 0.9% saline solution was injected in place of aroeira extract. Each group was divided into two subgroups (SGA and SGC) of 10 animals. According to their scheduled death, they were named subgroups SGA3 and SGC3 when killed in the 3th day after surgery and subgroups SGA7 and SGC7 in the 7th day. The abdominal cavity and the bladder suture were macroscopically evaluated. RESULTS: The microscopic analysis of the surgical incision in the bladder was performed by means of the hematoxilin-eosine stain and the trichrome of Masson. The macroscopic analysis showed an infection in the surgical incision in three rats of the SGC group and in one of the SGA, and peritoneal adhesion in the 26 rats belonging nine to SGC and 17 in the SGA. The microscopic evaluation revealed a more severe acute inflammation process in the SGC on the 3th (p=0.045) and in the 7th (0=0.002) days. In the SGA, a more significant colagenization (p=0.001) could be seen, as far as the evidence of vascular neoformation (p=0.002) on the 3th day. Chronic inflammatory reaction (p=0.006) and a more intense vascular neoformation (p=0.001) were observed in the 7th day. CONCLUSION: The hydroalcoholic aroeira extract have a favorable effect in the healing process of cystotomies done in rats.

PERITONITE FECAL EM RATOS: EFICÁCIA DA LAVAGEM DA CAVIDADE PERITONEAL COM SOLUÇÃO DE CLORETO DE SÓDIO A 0,9%

The aim of the present study is to analyze the influence of peritoneal lavage with saline solution in fecal peritonitis in rats. Thirty six Wistar rats were used, adult, male,weighing 160 to 210 g. The animals were allocated into three groups and submitted to peritonitis induced by homogenized human feces. In group I the procedure was carried out to verify the efficacy of the fecal peritonitis and all animals died after 24 hours of the intraperitoneal injection. After 6 hours of peritonitis evolution , the rats of the group II were submitted to laparotomy and irrigation of the abdominal cavity with saline solution. In this group all the animals were alive after 48 hours of the laparotomy. In group III the rats were submitted to laparotomy and cleaning of the peritoneal cavity with gauze. It was verify that only 6 rats were alive after 48 hours of the laparotomy.The present study demonstrated that the irrigation of the peritoneal cavity with saline solution was able to reduce the mortality rate in rats with fecal peritonitis.

Avaliação fitoterápica da Jatropha gossypiifolia L. na cicatrização de suturas na parede abdominal ventral de ratos

INTRODUCTION: The Jatropha gossypiifolia L., which is used in popular medicine is considered to have good diuretic effect in hypertension and is also used as a laxative drug. It seems to have a healing effect, although not proved till now. PURPOSE: To evaluate the influence of intraperitoneum administration of Jatropha Gossypiifolia L., in suture healing of ventral abdominal wall of rats, through tensiometric measurement, macro and microscopic aspect of post-operative period. METHODS: Forty wistar male rates were allocated in two groups of 20 animals . After the incision and exposure of abdominal cavity 1 ml/kg/weight of 0,9% sodium chloride solution was injected in control group, and in the other one the injection was of 1 ml/kg/weight of a gross ethanol extract of Jatropha gossypiifolia L. The suture of the abdominal wall was than performed with polypropylene separated stitches. The animals were followed-up and killed in the third and seventh days. The ventral abdominal wall was macroscopically analyzed, the resistance strength to strain was measured and it was also studied the histological aspects. RESULTS: On macroscopic examination more intense adhesion was found on the group of Jatropha in both third and seventh post-operative days. The strain evaluation was meanly greater on Jatropha group also in third and seventh days. CONCLUSION: The histological comparative analysis between the different groups showed that the acute inflammatory process was meanly greater for the Jatropha group in third and seventh post-operative days. The vascular neoformation was significantly greater in third pos-operative day of Jathopha group; the other histological parameters were just alike. The intraperitoneum injection of Jatropha extract did not have any significant improvement for the wound healing on ventral abdominal wall on the evaluated animals in this study, no matter if analyzed at the third or seventh pos-operative days.

Healing process in bladder suture of rats with and without the administration of intraperitoneal Jatropha gossypiifolia L. gross extract

INTRODUCTION The suture of tissues and their healing process is one of the basic fundamentals of surgery, and the research on substances which could improve the process is an ongoing challenge. The use of plants has been tested but till nowadays without scientific demonstration. PURPOSE To compare the macroscopic and histological alterations done by the use of intraperitoneal Jatropha gossypiifolia L. gross extract, in the healing process of sutures performed on the bladder of rats. METHODS Forty adult, male, Wistar rats were distributed into two animal groups. One centimeter longitudinal incision on the bladder ventral wall, and single plane synthesis with separated polyglactine 910 5-0 (Ethicon) stitches was done in all the animals. In the control group an intraperitoneal cavity instillation of distilled water at a ratio of 1 ml/kg/weight, and a 1 ml/kg/weight of Jatropha gossypiifolia L. extract for the Jatropha group was done representing 200 mg of the substance. Each group was subdivided into two with 10 animals in each, being submitted to euthanasia on 3rd and 7th post-operative days. Comparative histological, macroscopic and statistical analysis were undertaken between the subgroups. RESULTS Statistical significant difference was observed in the acute inflammation changes, vascular neoformation and bonding on day 3; the first one was greater in the control group, and the latter two greater in the Jatropha group; acute inflammation variables and fibroblastic proliferation presented to be more intense on day 7, with statistical significance favoring the control group. CONCLUSION No favorable healing effect was observed with the administration of single intraperitoneal dose of Jatropha gossypiifolia L. gross extract on the sutures of bladder in rats.

Translocação bacteriana: efeito de dieta imunoestimuladora em ratos com oclusão intestinal

The aim of this study was to analyze the occurrence of bacterial translocation following intestinal occlusion in rats and to verify the importance of a immune-enhancing diet in reducing the rate of bacterial translocation in these animals. Twenty-four adult, male Wistar rats were used, weighing 180 to 240g, divided into three groups of eight rats each. Group C (Control) received a rat chow group 1 (Immunmodulation) a immune-enhancing diet and group D ( Malnutrition) received the half of the same rat chow as group C. After seven days all rats were subjected to intestinal occlusion by ligation of the terminal ileum. After 18 hours, under strictly aseptic technique the abdomen was opened and 6 ml of blood was withdrawn from the inferior vena cava for the determination of the blood glucose, albumin and leukocyte count. The spleen, liver and mesenteric lymph node were removed separately to microbiologic study and a segment of the proximal jejunum for histologic study. Caloric intake was similar in groups C and I and half in group D. Blood glucose levels were lower in group D. The microbiologic culture of the mesenteric lymph nodes, spleen and liver were positive in all animals of group D, in 58.3% of group I and in 66.6% of the rats of the group C. Histological changes were minimal when the three groups were compared. It is concluded that bacterial translocation occurs in rats subjected to intestinal occlusion and that nutritional support with an immune-enhancing diet is able to reduce the rate of bacterial translocation in rats with intestinal occlusion.

Efeito de cálculos biliares humanos na cavidade peritoneal de ratos

The aim of the present study is to analize in an experimental study, the macroscopical and microscopical features of the human gallstones on the peritoneal cavity of rats. Thirty-two male Wistar rats, weighting between 205 to 268g, were used. The animals were divided into two groups. The surgical procedure was performed in each one group: in group A (n =16), the animals were underwent to intestinal mobilization; in group B (n =16), human gallstones were left on the peritoneal cavity. The rats were evaluated at postoperative days 21 and 42. The results showed that simple bowel mobilization caused adhesions on the animals (n =10). Adhesions were noted in 11 gallstones in group B. Histologically there was no exuberant fibrosis surrounding the gallstone, mainly at postoperative day 42. No macro or micro abscesses were noted, and there was no evidence of free intra peritoneal fluid. This study suggest that despite of low incidence of complications , every attempt should be made to retrieve stones lost during cholecystectomy.