Umezuruike Linus Opara

Colour Measurement and Analysis in Fresh and Processed Foods: A Review

Colour is an important quality attribute in the food and bioprocess industries, and it influences consumer’s choice and preferences. Food colour is governed by the chemical, biochemical, microbial and physical changes which occur during growth, maturation, postharvest handling and processing. Colour measurement of food products has been used as an indirect measure of other quality attributes such as flavour and contents of pigments because it is simpler, faster and correlates well with other physicochemical properties. This review discusses the techniques and procedures for the measurement and analysis of colour in food and other biomaterial materials. It focuses on the instrumental (objective) and visual (subjective) measurements for quantifying colour attributes and highlights the range of primary and derived objective colour indices used to characterise the maturity and quality of a wide range of food products and beverages. Different approaches applied to model food colour are described, including reaction mechanisms, response surface methodology and others based on probabilistic and non-isothermal kinetics. Colour is one of the most widely measured product quality attributes in postharvest handling and in the food processing research and industry. Apart from differences in instrumentation, colour measurements are often reported based on different colour indices even for the same product, making it difficult to compare results in the literature. There is a need for standardisation to improve the traceability and transferability of measurements. The correlation between colour and other sensory quality attributes is well established, but future prospects exist in the application of objective non-destructive colour measurement in predictive modelling of the nutritional quality of fresh and processed food products.

NIR Spectroscopy Applications for Internal and External Quality Analysis of Citrus Fruit—A Review

The global citrus industry is continually confronted by new technological challenges to meet the ever-increasing consumer awareness and demand for quality-assured fruit. To face these challenges, recent trend in agribusiness is declining reliance on subjective assessment of quality and increasing adoption of objective, quantitative and non-destructive techniques of quality assessment. Non-destructive instrument-based methods are preferred to destructive techniques because they allow the measurement and analysis of individual fruit, reduce waste and permit repeated measures on the same item over time. A wide range of objective instruments for sensing and measuring the quality attributes of fresh produce have been reported. Among non-destructive quality assessment techniques, near-infrared (NIR) spectroscopy (NIRS) is arguably the most advanced with regard to instrumentation, applications, accessories and chemometric software packages. This paper reviews research progress on NIRS applications in internal and external quality measurement of citrus fruit, including the selection of NIR characteristics for spectra capture, analysis and interpretation. A brief overview on the fundamental theory, history, chemometrics of NIRS including spectral pre-processing methods, model calibration, validation and robustness is included. Finally, future prospects for NIRS-based imaging systems such as multispectral and hyperspectral imaging as well as optical coherence tomography as potential non-destructive techniques for citrus quality assessment are explored.

Modified Atmosphere Packaging of Pomegranate Fruit and Arils: A Review

Ongoing global drive for a healthier diet has led to a rise in demand for convenient and fresh food produce, with high nutritional value and free of additives. Minimally fresh processed fruits and vegetables, satisfies the consumers’ perception of a high nutritional quality and convenience produce. Minimally processed fruit and vegetables are susceptible to increased deterioration in quality and microbial infestation due to increase in endogenous enzymatic processes and respiration rate. Modified atmosphere packaging (MAP) technology offers the possibility to retard produce respiration rate and extend the shelf life of fresh produce. However, it is important to correlate the permeability properties of the packing films with the respiration rate of the produce, in order to avoid anaerobic conditions which could lead into fermentation of produce and accumulation of ethanol. Hence, mathematical prediction modelling is now widely applied in the design and development of effective MAP technology in both whole and minimally processed fresh produce. With increasing global interest in postharvest handling and nutrition value of pomegranate, MAP of minimally processed pomegranate arils offers additional innovative tool for optimal use and value addition, including the utilization of lower-grade fruit with superficial peel defects such as; cracks, splits, and sunburnt. This review paper highlights the current status and applications of modified atmosphere packaging in whole fruit and minimally processed pomegranate arils and identifies future prospects.

Modified atmosphere packaging technology of fresh and fresh-cut produce and the microbial consequences ? A review

Modified atmosphere packaging (MAP) technology offers the possibility to retard the respiration rate and extend the shelf life of fresh produce, and is increasingly used globally as value adding in the fresh and fresh-cut food industry. However, the outbreaks of foodborne diseases and emergence of resistant foodborne pathogens in MAP have heightened public interest on the effects of MAP technology on the survival and growth of pathogenic organisms. This paper critically reviews the effects of MAP on the microbiological safety of fresh or fresh-cut produce, including the role of innovative tools such as the use of pressurised inert/noble gases, predictive microbiology and intelligent packaging in the advancement of MAP safety. The integration of Hazard Analysis and Critical Control Points-based programs to ensure fresh food quality and microbial safety in packaging technology is highlighted.

Antibacterial, antioxidant and tyrosinase-inhibition activities of pomegranate fruit peel methanolic extract.

BackgroundThis study evaluated, using in vitro assays, the antibacterial, antioxidant, and tyrosinase-inhibition activities of methanolic extracts from peels of seven commercially grown pomegranate cultivars.MethodsAntibacterial activity was tested on Gram-positive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli and Klebsiella pneumonia) using a microdilution method. Several potential antioxidant activities, including radical-scavenging ability (RSA), ferrous ion chelating (FIC) and ferric ion reducing antioxidant power (FRAP), were evaluated. Tyrosinase enzyme inhibition was investigated against monophenolase (tyrosine) and diphenolase (DOPA), with arbutin and kojic acid as positive controls. Furthermore, phenolic contents including total flavonoid content (TFC), gallotannin content (GTC) and total anthocyanin content (TAC) were determined using colourimetric methods. HPLC-ESI/MSn analysis of phenolic composition of methanolic extracts was also performed.ResultsMethanolic peel extracts showed strong broad-spectrum activity against Gram-positive and Gram-negative bacteria, with the minimum inhibitory concentrations (MIC) ranging from 0.2 to 0.78 mg/ml. At the highest concentration tested (1000 μg/ml), radical scavenging activities were significantly higher in Arakta (83.54%), Ganesh (83.56%), and Ruby (83.34%) cultivars (P< 0.05). Dose dependent FIC and FRAP activities were exhibited by all the peel extracts. All extracts also exhibited high inhibition (>50%) against monophenolase and diphenolase activities at the highest screening concentration. The most active peel extract was the Bhagwa cultivar against monophenolase and the Arakta cultivar against diphenolase with IC50 values of 3.66 μg/ml and 15.88 μg/ml, respectively. High amounts of phenolic compounds were found in peel extracts with the highest and lowest total phenolic contents of 295.5 (Ganesh) and 179.3 mg/g dry extract (Molla de Elche), respectively. Catechin, epicatechin, ellagic acid and gallic acid were found in all cultivars, of which ellagic acid was the most abundant comprising of more than 50% of total phenolic compounds detected in each cultivar.ConclusionsThe present study showed that the tested pomegranate peels exhibited strong antibacterial, antioxidant and tyrosinase-inhibition activities. These results suggest that pomegranate fruit peel could be exploited as a potential source of natural antimicrobial and antioxidant agents as well as tyrosinase inhibitors.

Design of Packaging Vents for Cooling Fresh Horticultural Produce

This review focuses on the design of vents in packages used for handling horticulture produce. The studies on vent designs that are conducted to obtain fundamental understanding of the mechanisms by which different parameters affect the rate and homogeneity of the airflow and the cooling process are presented. Ventilated packages should be designed in such a way that they can provide a uniform airflow distribution and consequently uniform produce cooling. Total opening area and opening size and position show a significant effect on pressure drop, air distribution uniformity and cooling efficiency. Recent advances in measurement and mathematical modelling techniques have provided powerful tools to develop detailed investigations of local airflow rate and heat and mass transfer processes within complex packaging structures. The complexity of the physical structure of the packed systems and the biological variability of the produce make both experimental and model-based studies of transport processes challenging. In many of the available mathematical models, the packed structure is assumed as a porous medium; the limitations of the porous media approach are evident during vented package design studies principally when the container-to-produce dimension ratio is below a certain value. The complex and chaotic structure within horticultural produce ventilated packages during a forced-air precooling process complicates the numerical study of energy and mass transfer considering each individual produce. Future research efforts should be directed to detailed models of the vented package, the complex produce stacking within the package, as well as their interaction with adjacent produce, stacks and surrounding environment. For the validation of the numerical models, the development of better experimental techniques taking into account the complex packaging system is also very important.

Transpiration rate and quality of pomegranate arils as affected by storage conditions

This study investigated the transpiration rate (TR) of pomegranate (Punica granatum L.) arils under various combinations of temperature (5, 10 and 15°C) and relative humidity (RH) (76, 86 and 96%) during storage. Transpiration rateTR ranged from 1.14 to 16.75 g/kg day across the various combinations of RH and temperature studied. Relative humidityRH had the most significant impact on TR (p < 0.05). Transpiration rateTR increased six-fold when RH was reduced from 96 to 76%, and correlated well with water vapour pressure deficit (WVPD) (R 2 = 96.1%). Aril weight loss increased at higher WVPD. After 8 days of storage, losses in quality attributes of arils were higher with increasing storage temperature and lowering RH. A mathematical model to predict TR as a function of temperature and RH was developed and successfully validated at 8°C. The target water vapour transmission rate of packaging materials for pomegranate arils was found to be 33 to 68 g/m2 day.

Analysis of genetic diversity in banana cultivars (Musa cvs.) from the South of Oman using AFLP markers and classification by phylogenetic, hierarchical clustering and principal component analyses

Banana is an important crop grown in Oman and there is a dearth of information on its genetic diversity to assist in crop breeding and improvement programs. This study employed amplified fragment length polymorphism (AFLP) to investigate the genetic variation in local banana cultivars from the southern region of Oman. Using 12 primer combinations, a total of 1094 bands were scored, of which 1012 were polymorphic. Eighty-two unique markers were identified, which revealed the distinct separation of the seven cultivars. The results obtained show that AFLP can be used to differentiate the banana cultivars. Further classification by phylogenetic, hierarchical clustering and principal component analyses showed significant differences between the clusters found with molecular markers and those clusters created by previous studies using morphological analysis. Based on the analytical results, a consensus dendrogram of the banana cultivars is presented.

Antioxidant contents of pre-packed fresh-cut versus whole fruit and vegetables.

Purpose – The purpose of this paper is to evaluate the difference in antioxidant contents of pre‐packed fresh‐cut and whole fruit and vegetables as sold in the market.Design/methodology/approach – Samples of pre‐packed fresh‐cut fruit and vegetables as well as whole produce were collected from the market in Muscat and Oman, and analyzed for vitamin C, lycopene and total carotenoids. Analysis of variance was carried out to determine the level of statistical differences between fresh‐cut and whole fruit and vegetables.Findings – In both fruit and vegetables, vitamin C contents are higher in whole than fresh‐cut produce, with greater reductions in vitamin C contents of fresh‐cut vegetables than fruit. In both fresh‐cut and whole fruit, lycopene content is 30‐36 times higher in watermelon than the contents of other fruit genotypes studied. Similarly, total carotenoids content of watermelon is six to 21 times higher than other types of fruit studied. Both lycopene and total carotenoids content are higher in wh...

Phytochemical content, antioxidant capacity and physicochemical properties of pomegranate grown in different microclimates in South Africa

We investigated the antioxidant and physicochemical properties of pomegranate (Punica granatum cv. Bhagwa) fruit grown in three microclimates in South Africa. The physicochemical properties of fruit peel and arils differed among the growing locations, including weight and redness color of both peel and arils. Contents of vitamin C, anthocyanin and total phenolics were 0.67–1.41 mg ml−1, 0.07–0.16 mg ml−1 and 8.54–13.91 mg ml−1, respectively. Fruit grown in locations with high light intensity had higher vitamin C content and lower phenolic content than those of fruit from low-altitude locations and high light intensity. The pH of juice did not significantly differ among locations, whereas significant variation was observed in total soluble solids (TSS), titratible acidity (TA) and TSS:TA ratio. Fruit from locations with low rainfall had high TSS and low TA. Principal component analysis was used successfully to classify fruit by growing location on the basis of antioxidant and phytochemical contents.