Umi Cahyaningsih

Molecular evidence of natural hybridization between Fasciola hepatica and F. gigantica

Nucleotide sequences of two regions, cytochrome c-oxidase subunit 1 (CO1) and NADH dehydrogenase subunit 1 (ND1) of the mitochondrial DNA and two regions, internal-transcribed spacer 2 (ITS2) and the D2 region in the 28S rDNA (28S) of the nuclear DNA were obtained from five Korean worms of the genus Fasciola in order to elucidate their taxonomic status. The CO1 and ND1 regions are all monomorphic in the Korean worms and similar to those of F. gigantica. On the other hand, the ITS2 and D2 regions were found to be polymorphic; that is, out of five worms, two possessed a F. gigantica-type sequence, one, a F. hepatica-type sequence and two possessed sequences of both types indicating an existence of different alleles at the loci. It should be noted that these variations of the ITS2 and D2 regions co-occur at the same individual worms. This was confirmed by sequencing five to six cloned PCR products for each worm. The present study strongly suggests interspecific cross-hybridization between the two species coexisting in Korea.

ENDOPARASIT CACING PADA ORANGUTAN EX-CAPTIVE DI SUAKA MARGASATWA SUNGAI LAMANDAU KALIMANTAN TENGAH INDONESIA

Penelitian ini bertujuan melakukan identifikasi dan mengetahui persentase orangutan yang terinfeksi endoparasit (cacing) pada orangutan excaptive yang berada di Suaka Margasatwa Sungai Lamandau. Sampel feses orangutan yang diperiksa sebanyak 30 individu yang didapatkan di camp Gemini dan Siswoyo. Pemeriksaan feses dilakukan secara makroskopis dan mikroskopis. Pemeriksaan mikroskopis dilakukan dengan menggunakan dua metode yaitu metode Ridley dengan larutan sodium acetate, acetic acid, dan formaldehyde (SAF) fiksatif dan metode apung. Hasil pengamatan menunjukkan adanya infeksi cacing gastrointestinal sebesar 56,7%. Hasil penelitian menunjukkan bahwa orangutan positif terinfestasi telur cacing tipe ascarid; trichurid; dan strongiloid masing-masing sebesar 43,3; 16,7; dan 6,7%. Orangutan dapat mengalami infeksi lebih dari satu jenis tipe telur cacing.

Protozoa Parasitik pada Ikan Sidat (Anguilla spp.) Asal Danau Lindu, Sulawesi Tengah

Infection protozoan parasitic on eels ( Anguilla spp.) from Lindu Lake was affected weight eels and health eels. The protozoan parasitic examinations were collection of eels, inspection eels, observation, measurement, identification of parasites, and staining protozoan used giemsa stained, trematode used semichons’s acetocarmine stained and nematode used clove oil and KOH. The diversity of parasitic protozoan found such as: Myxidium sp., Myxobolus sp., Chilodonella sp., Ceratomyxa sp., Balantidium sp., Henneguya sp., and Glugea sp. The highest prevalence Myxidium sp. in eels was 77% and no protozoan dominated between other parasites. Based on chi-square test, protozoan parasitic had a different preference to organs of eels.