Umran Seven Erdemir

Fractionation analysis of manganese in Turkish hazelnuts (Corylus avellana L.) by inductively coupled plasma-mass spectrometry.

In this study, an analytical fractionation scheme based on water, diethyl ether, n-hexane, and methanol extractions has been developed to identify manganese-bound fractions. Additionally, in vitro simulated gastric and intestinal digestion, n-octanol extraction, and activated carbon adsorption were used to interpret the manganese-bound structures in hazelnuts in terms of bioaccessibility. The total content of manganese in the samples was determined by inductively coupled plasma-mass spectrometry after microwave-assisted digestion, and additional validation was performed using atomic absorption spectroscopy. Water fractions were further evaluated by high-performance liquid chromatography hyphenated to inductively coupled plasma-mass spectrometry for the identification of water-soluble manganese fractions in hazelnut samples. The limits of detection and quantification were 3.6 and 12.0 μg L(-1), respectively, based on peak height.

Fractionation analysis and bioavailability of manganese in spinach (Spinacia oleracea L.) leaves

Abstract This paper introduces a fractionation scheme using water, acetone, chloroform, diethyl ether, ethanol, n-hexane, and methanol as extractants for the determination of manganese in spinach samples by inductively coupled plasma-mass spectrometry (ICP-MS). Simulated gastric and intestinal digestions as well as n-octanol extraction and activated carbon adsorption were performed for the bioavailability assessments. Comparative studies of the various extraction treatments were evaluated for confirmation analysis. The total elemental concentrations were determined after digesting the samples in a microwave digestion system. The method validation parameters were defined in terms of the detection limits, accuracy, and precision. Additional validation was performed by comparing the ICP-MS method with atomic absorption spectrometry. The limits of detection and quantification were 0.046 and 0.154 mg kg-1, respectively. Additionally, the repeatability and reproducibility, calculated from the relative standard deviation (%RSD), were 2.4% and 3.7%, respectively.

Ni-induced Changes in Nitrate Assimilation and Antioxidant Metabolism of Verbascum olympicum Boiss.: Could the Plant be Useful for Phytoremediation or/and Restoration Purposes?

Verbascum olympicum Boiss. (Scrophulariaceae) were studied as a candidate plant for remediating the Ni polluted soils. The metabolic responses, such as nitrate assimilation (nitrate reductase and glutamine synthetase activity) and antioxidant system activity [superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activity], of this species exposed to nickel in Hoaglands nutrient medium were investigated as remediation performance parameters. The accumulation of nickel and the variations in the content of some elements (B, Cu, Fe, Mg, Mn, Mo and Zn) and some growth parameters, such as the water content, biomass production, and contents of chlorophyll and soluble protein, were also examined. The accumulation of Ni in both the roots and leaves varied depending on the exposure times and doses. Increased oxidative stress was suggested by the increases in the activities of SOD, CAT and APX. Although some element contents were inhibited by Ni treatments, these inhibitory effects was decreased depending on the time, and even these elements are accumulated in roots. These results are the novelties in the use of this species in biotechnology.

Characterization of Copper Bioavailability in Wheat Flour by Chemical Fractionation and Inductively Coupled Plasma–Mass Spectrometry

ABSTRACT The bioavailability of copper in wheat flour is reported based on chemical fractionation using water, diethyl ether, n-hexane, methanol, acetone, and chloroform/methanol to identify copper-bound matrix sites. The total copper concentrations in the fractions were determined by inductively coupled plasma-mass spectrometry. In vitro gastrointestinal digestion was performed using pepsin and pancreatin enzyme solutions at defined pH values for bioavailability studies. The bioavailable forms of copper in the samples were 61–80% of the total concentration. Extraction with n-octanol and activated carbon adsorption studies were evaluated to compare their efficiencies to the enzymatic approach.

Variation in trace element mobility and nitrogen metabolism of Verbascum olympicum Boiss. under copper stress

The aim of this study was to evaluate the effects of copper (Cu) stress on accumulation and transport of trace elements, nitrogen assimilation, and growth parameters of Verbascum olympicum. Eight-week-old seedlings were grown in Hoaglands solution and exposed to 0, 50, 250, or 500 µM CuSO4 for seven days in laboratory conditions. Bioaccumulation of trace elements (boron, bismuth, cobalt, Cu, iron, lithium, manganese, molybdenum, nickel, lead, zinc) in the roots and leaves was determined by inductively coupled plasma–mass spectrometry after one, three, and seven days. Chlorophyll content, nitrate reductase, and glutamine synthetase activities, soluble protein content, and biomass were determined. Copper accumulated in the roots and leaves (up to 19609.8 and 256.2 mg kg−1 dry weight, respectively). Other trace elements accumulated to higher levels in the roots of Cu-treated plants compared with those of control plants. High Cu concentrations decreased nitrogen-assimilatory enzyme activities. Compared with control plants, those treated with high Cu concentrations showed lower chlorophyll contents, total protein contents, and biomass. Nitrogen assimilation and growth parameters of V. olympicum were negatively affected by Cu treatment but mineral nutrition was not severely disrupted. The results support the suitability of V. olympicum as a candidate for phytoremediation of Cu-contaminated soils.

An alternative method for screening of Sudan dyes in red paprika paste by gas chromatography-mass spectrometry

An indirect analytical screening method for Sudan dyes by gas chromatography mass spectrometry was developed as an alternative method to novel liquid chromatography mass spectrometry. Red paprika paste samples were extracted with acetone and centrifuged at room temperature. The supernatant was removed from the solution and buffered. The reduction step is performed chemically with sodium dithionite and corresponding dyes as amine products were identified by GC-MS. The method was validated for analytical properties using spiked paste samples. Several parameters which may influence the reduction procedure, such as temperature and the amount of reducing agent, were optimized using central composite design methodologies for Sudan III in order to increase its lower apparent recovery as compared with the others. Optimum conditions for Sudan III were found to be 45 °C and 1.3 M by using a central composite design. The apparent recovery values were 77.6%, 69.4%, 51.3%, 42.5% with relative standard deviations of 31.8%, 20.4%, 20.3%, 31.2% for Sudan I, II, III, IV dyes respectively. This method was used successfully for screening of Sudan dyes in paprika paste products by GC-MS.

Correlation of lithium bioaccessibility from tea (Camellia sinensis L.) with tea type and consumption habits

In this study, the total contents, leachability into tea infusions, and bioaccessibility of lithium from black, Earl Grey, and green teas were evaluated by inductively coupled plasma mass spectrometry. Leachabilities were evaluated after infusion for 2, 5, or 10min. Bioaccessibility was determined in vitro under simulated stomach and intestinal digestion conditions. Addition of lemon juice, sugar, or milk for consumption, and calcium, tannic acid, and citric acid as additives were evaluated to determine if they affected bioaccessibility of lithium from black tea. The bioaccessible lithium contributed to 0.01%, 0.02%, and 0.03% of the recommended dietary allowances of lithium for black, Earl Grey, and green tea samples, respectively. These contributions may increase up to 4.4 times or decrease up to seven times with certain additives.

Assessment of Trace Elements in Plantago holosteum Scop. (Plantaginaceae) from Abandoned Tungsten Mine Works Using Inductively Coupled Plasma–Mass Spectrometry

ABSTRACT The influence of tungsten on the uptake and translocation of essential elements (B, Bi, Cd, Co, Cr, Cu, Fe, Mn, Mo, Pb, Zn, and W) of Plantago holosteum around the abandoned tungsten mine works on Uludağ Mountain, Turkey was evaluated. Open-wet and Kjeldahl digestion procedures were applied to the samples before elements were determined by inductively coupled plasma–mass spectrometry. Accuracy was evaluated using standard tea and spinach leaf and soil-certified reference materials. The novel approach of this work identified P. holosteum as a possible candidate bioaccumulator species, which was widespread in the tungsten mining area and provided overall, realistic evaluations for many elements, particularly W. Thus, the specific behavior of the selected species separated this species from the others in this damaged ecosystem. The W concentrations in soils and plants were in the range from 6 to 2591 mg kg−1 dry weight and 0.6 to 85.3 mg kg−1 dry weight, respectively. The concentrations of elements in soil samples from the waste removal pool were higher than those from the unpolluted sites, and all elements except manganese increased in plant samples.

Lead Fractionation Analysis in Lipstick Samples by Inductively Coupled Plasma-Mass Spectrometry

A new analytical approach based on fractionation was introduced for lead in lipstick samples. Different separation techniques including n-hexane, glycerol extraction, and activated carbon adsorption were used to characterize the lipid fraction, polar and aromatic components of the samples. Additionally, artificial saliva and food stimulant extractions were used for the risk assessment studies. Trace metal levels in fractions were determined by inductively coupled plasma-mass spectrometry. Method validation parameters in the total element determinations were defined in terms of detection limits, accuracy, and precision. The limits of detection and quantification were 0.02 and 0.07 mg kg−1 for Pb; whereas the repeatability and reproducibility of the results based on percent relative standard deviation were 3.0% and 7.2% for lead, respectively.

Assessing the Bioaccessibilities of Some Elements in Fruit Based Complementary Baby Foods

In this study, total concentrations and bioaccessibility of some essential and toxic elements (As, Cd, Cr, Cu, Fe, Mg, Mn, Ni, Pb and Zn) were determined in commercial fruit purees intended for baby consumption as complementary foods. Enzymatic in vitro digestions were performed to simulate the stomach and the intestine conditions. A five-level, three factor central composite design, was applied to optimize the open-wet digestion methodology as well as enzyme amounts used in vitro methods to achieve maximum elemental levels. Rice flour and baby food composite certified reference materials analysis were applied to optimize the digestion parameters and evaluate the accuracy of the optimized method. Water fractions of samples were analyzed and evaluated in terms of their suitability for risk assessment studies by inductively coupled plasma mass spectrometry. Recommended dietary allowance level of 1.3% for Zn, adequate intake levels of 15.3%, 0.04%, 4.4% and 68% for Cu, Mg, Mn and Cr, respectively were achieved as well as 20%, 6% level and 2% tolerable intakes of Cd, Ni and As, respectively. Thus, contribution of the consumption of one jar of fruit based complementary baby food to diet of infants aged up to 12 months were assessed.