Uppeandra Dhar

Setting priorities for conservation of medicinal plants –– a case study in the Indian Himalaya

The paper analyses available information on various aspects of medicinal plants (MPs) of the Indian Himalaya region (IHR). Among the identified gaps in knowledge, lack of objective assessment of threats was considered a major impediment in setting conservation priorities. The paper addresses this issue keeping in view the needs of users (industry) and conservation concerns of academics (biologists). An approach for prioritisation of MPs for conservation was thus developed. Prioritisation based on three indices: (i) use value index (UVI) indicate threats imposed by users, (ii) sensitivity index (SI) reflect conservation concerns of biologists, and (iii) importance value index (IVI) is the cumulative value of (i) and (ii) to prevent biased approach. Dependence of pharmaceutical industry on only 10% of the available stock and that too on non-native elements suggest that the potential of true Himalayan MPs has not been harnessed as yet. However, increased dependence on exclusive wild forms (64.6%), dominance of destructive harvest trend (69%) and restricted distribution range of most MPs used by the industry is a pointer to the intensity of threat. The paper identifies 20 top ranking MPs for conservation in each life form.

Prioritization of medicinal plants on the basis of available knowledge, existing practices and use value status in Uttaranchal, India

In order to understand the pattern of indigenous uses of medicinal plants available in the Uttaranchal state of the Indian Himalaya, this study was undertaken through literature survey and fieldwork in various parts of the state. A list of all the major and most of the lesser categories of ailments was prepared and categorized with the help of medical practitioners. A total of 300 plant species used in curing 114 ailments prevailing in various ethnic and non-ethnic communities of Uttaranchal were documented. These 114 ailments were further grouped into 12 broad classes of diseases in order to project the indigenous uses of medicinal plants for various ailments. It was found that herbs contributed the highest number of medicinal plants (65%), followed by shrubs (19%) and trees (16%). The maximum number of plant species were used to cure generalized body aches and colic, followed by gastrointestinal and dermatological problems. Vitex negundo was the most important species, used for the treatment of more than 48 ailments. Azadirachta indica, Woodfordia fruticosa, Centella asiatica, Aegle marmelos, Cuscuta reflexa, Butea monosperma, Phyllanthus emblica, and Euphorbia hirta were among other important medicinal plants based on their high use values. The underground parts of the plant were used in the majority of cases. Of 300 medicinal plants, 35 were rare and endangered species, of which about 80% was restricted to the high altitude alpine region of Uttaranchal Himalaya. A priority list of 17 medicinal plant species was prepared on the basis of endemism, use value, mode of harvesting and rarity status. Strategies for long-term conservation of these valuable medicinal plants are discussed.

Structural diversity and representativeness of forest vegetation in a protected area of Kumaun Himalaya, India: implications for conservation

Forest vegetation of a Protected Area (Askot Wildlife Sanctuary) in Kumaun (west Himalaya) was analysed for structure, composition and representativeness. A high percentage of non-natives was noticed in herbaceous flora of all representative forest types. Floristic representativeness in all growth forms (tree, shrub and herbs) increased significantly (tree, p<0.05; shrub and herbs, p<0.01) with altitude. The population structures of trees suggest, (i) expansion of riverine and Pinus roxburghii forests; (ii) compositional changes in Quercus leucotrichophora and Quercus lanuginosa forests, largely owing to preferred extraction demand of inhabitants; and (iii) infrequent regeneration and declining population of Quercus semecarpifolia and Abies pindrow forests. The possible pathways of non-native introduction in the study area were examined. Considering the existing status of forest vegetation and future trends, proliferation of non-native species in most forest types was discussed. It is suggested that the compositional changes vis-a`-vis proliferation of non-native species need priority attention while initiating conservation activities in the reserve.

ORGANOGENESIS, EMBRYOGENESIS, AND SYNTHETIC SEED PRODUCTION IN ARNEBIA EUCHROMA - A CRITICALLY ENDANGERED MEDICINAL PLANT OF THE HIMALAYA

SummaryThis is the first report of simultaneous organogenesis and somatic embryogenesis in Arnebia euchroma, a highly valued, critically endangered medicinal plant of the Himalaya. Root-derived callus showed only rhizogenesis, whereas leaf-derived callus showed simutaneous organogenesis and somatic embryogenesis. Organogenesis was optimal (12.2 shoots per culture) in 1 μM indole-3-butyric acid combined with 2.5 μM 6-benzyladenine and induction of somatic embryogenesis (16.3 embryos per culture) occurred in 2.5 μM indole-3-butyric acid combined with 2.5 μM 6-benzyladenine. Shoots rooted (100%) best in half-strength Murashige and Skoog (MS) medium supplemented with 2.0 μM indole-3-butyric acid. Early cotyledonary-stage embryos encapsulated with 3% sodium alginate and calcium nitrate (100 mM for 25 min) showed 60.6% germination in MS medium. Rooted shoots transferred to a mixture of sterile soil, sand, and peat (1∶1∶1 by volume) showed 72% survival ex vitro. Application of these protocols would be helpful in reducing pressure in natural populations, in genetic transformation studies, and in long-term storage of elite genotypes through synthetic seed production.

Micropropagation of Indian wild strawberry.

An efficient method of micropropagation based on an increased percentage survival of explants and reduced phenol-induced browning in wild strawberry has been developed. Serial transfer of nodal explants was carried out at 24-, 48- and 96-h intervals. Nodal segments cultured on Murashige and Skoog medium supplemented with 6-benzyladenine (4.0 μM) and α-naphthalene acetic acid (0.1 μM) gave the best (94.4%) explant establishment and shoot number (22.3) per explant. Of the cytokinins tested, 6-benzyladenine was found more effective than kinetin and N6-(γ,γ dimethylallyamino) purine. Excised shoots rooted on half-strength agar-gelled medium with 1.0 μM α-naphthalene acetic acid. Rooted shoots with fully expanded leaves acclimatized successfully and about 70% of plantlets survived ex vitro.

Factors controlling micropropagation of Myrica esculenta buch. – Ham. ex D. Don: a high value wild edible of Kumaun Himalaya

Various factors such as browning, season, media type and plant growth regulators influence the micropropagation of female trees of Myrica esculenta . These factors have successfully been addressed after addition of some media additives, collection of the explant at right season, standardizing the media type and use of plant growth regulators at varying concentrations. Polyvinylpyrollidone (PVP - 0.5%) was found effective for successful partial removal of phenolic compounds and obtaining maximum percent survival of explants. The explant collection season played an important role in reducing phenol induced browning and winter season was found best for explant establishment. The maximum number of shoots (4-5/explant) was obtained in Woody Plant Medium (WPM) supplemented with 10µM kinetin and 0.1µM naphthalene acetic acid (NAA). Of all the media types tried, Woody Plant Medium was found to be the best. Kinetin was found superior to benzyl amino purine and N 6 (γ,γ-dimethylallyamino)purine (2iP) for explant establishment and multiplication. NAA induced rooting to 45.8% of explants in 1/2 strength Woody Plant Medium. However, ex vitro survival percentage was low. Key Words: Browning, in vitro, plant regeneration, tissue culture, woody plant medium. African Journal of Biotechnology Vo l.3(10) 2004: 534-540

Assessment of fuel resource diversity and utilization patterns in Askot Wildlife Sanctuary in Kumaun Himalaya, India, for conservation and management

A general decrease in abundance of wild plant species used as sources of fuel suggests that more detailed information is urgently needed on species-level trends and their conservation. Such studies have not been carried out so far in India and elsewhere; we therefore quantified the species-wise extraction of fuel from a site (Gori Ganga Valley) in Askot Wildlife Sanctuary in the Kumaun Himalaya. In all, 31 species (26 trees and 5 shrubs) were used as fuel, of which 14 were native to the Himalaya. Utilization patterns, distributions, probabilities of use (PU), resource use indices (RUI), preferences and availabilities in forest communities of these species were determined. Use pattern did not vary much amongst low altitude villages (Similarity: 52–74%), whereas along the vertical (elevational) gradient it varied considerably (Similarity: 15–31%). Woodfordia fruticosa (L.) Kurz, Pinus roxburghii Sarg., Quercus leucotrichophora A.Camus, Macaranga pustulata King ex Hk. f., Quercus lanuginosa Don, Engelhardtia spicata Bl. and Mallotus philippensis (Lamk.) Muell. contributed most to collections, while Pyracantha crenulata (Don) Roem., Syzygium cuminii (L.) Skeels, Alnus nepalensis Don and Bauhinia vahlii Wt. & Arn. were in lesser demand. W. fruticosa , P. roxburghii , M. pustulata , Casearia elliptica Willd., E. spicata , M. philippensis , Q. leucotrichophora and Phoebe lanceolata (Nees) Nees showed high values of PU and RUI, indicating high pressure. High density of P. roxburghii , Rhododendron arboreum Sm., Q. lanuginosa , Q. leucotrichophora , Lyonia ovalifolia (Wall.) Drude, C. elliptica and M. pustulata amongst trees and Maesa indica A.DC., P. crenulata and W. fruticosa amongst shrubs exhibited high density but the remaining species showed low density indicating their possible depletion. Intensive management of natural habitats of species highly-preferred for fuel, diversification of choice of species from natives to non-natives, large scale propagation of highly preferred taxa and plantation of seedlings in the degraded, uncultivated and marginal lands through peoples’ participation should promote conservation and management of fuel resources.

Micropropagation of Pittosporum napaulensis (DC.) Rehder & Wilson - a rare, endemic Himalayan medicinal tree.

An in vitro propagation protocol has been developed from mature trees of Pittosporum napaulensis. The best bud proliferation (83.1%), shoot number (21 axillary shoots/ explant) and shoot length (5.5 cm) was achieved in Murashige and Skoog (MS) medium supplemented with 5.0 μM N−6 benzyladenine and 0.1 μM α- naphthalene acetic acid. Of the three cytokinins tested (N−6 benzyladenine, kinetin and thidiazuron), N−6 benzyladenine proved to be the best for shoot induction. Shoot regeneration potential varied among genotypes. Regenerated shoots rooted after 48 hours treatment on half-strength MS liquid medium supplemented with 20 μM indole-3-butyric acid. Rooted shoots transferred to 120 g (w/v) soilrite + sand + soil (1:1:1) mixture showed 70% survival. Twenty-one plantlets are growing well in green house conditions.

Micropropagation ofBauhinia vahlii Wight & Arnott - a leguminous liana.

Anin vitro propagation protocol for a leguminous liana,Bauhinia vahlii, has been established. In the first experiment, cotyledonary nodes fromin-vitro-germinated seedlings were cultured on various basic media (Murashige and Skoog medium, Woody Plant medium, B5, and 1/2 Murashige and Skoog medium) containing 1.0μM thidiazuron. Shoot proliferation (96.20%) and multiplication (5.55 shoots/explant) was best when cultured on Murashige and Skoog medium. The second experiment compared responses to benzylaminopurine, kinetin, zeatin and thidiazuron. Murashige and Skoog medium supplemented with 1.0μM thidiazuron proved most effective for both shoot proliferation and shoot multiplication. The effect of cytokinin type and concentration and their interaction was found to be significant (P<0.001) for explant proliferation, shoot number and length. Subsequent rooting (55.14%) of the regenerated shoots was achieved on half-strength Murashige and Skoog medium supplemented with IμM 1- naphthaleneacetic acid. Successful transfer of regenerants to soil has been accomplished, and efforts are being made to gradually transfer them to field conditions.

The availability, fruit yield, and harvest of Myrica esculenta in Kumaun (West Himalaya), India.

Abstract Myrica esculenta Buch.-Ham. ex D. Don is a popular, potentially income-generating wild edible in the Indian Himalaya. The species prefers Pinus roxburghii Sarg., Quercus leucotrichophora A. Cam., and mixed Quercus forests, contributing 15–26% of total tree density in the forests. It performs best in Pinus roxburghii forests, where its density correlates with Pinus tree biomass. The regeneration of Myrica is poor in all the habitats. However, recruitment of species increases consistently from abundant Myrica to no-Myrica stands. The fruit yield increases with tree size category and differs between habitats. The potential yield at different sites is 2.0–4.2 tonnes/ha, of which 2.8–7.2% is harvested for income generation. The income generated from Myrica fruit is significant, considering the regional annual per capita income. The possible impact of fruit harvesting and other disturbance factors on the regeneration of the species is discussed. There are significant options for enhancing the income-generating potential through value addition.