Uttam Datta

Morphological study of the cytoplasmic droplets as an index of sperm maturation in Black Bengal buck (Capra hircus)

La ubicacion de los droplets citoplasmicos (CD) y sus dimensiones variaron significativamente (p <0,01) cuando las celulas espermaticas atraviesan a traves de las regiones de cabeza, cuerpo y cola de epididimo y vas deferens respectivamente. La disminucion gradual en la morfologia de los CD entre las regiones del epididimo se relacionaron de forma significativa (p <0,01, p <0,05). El desplazamiento caudal de las CD, junto con la regresion en el tamano y, finalmente, su exclusion desde los espermatozoides refleja uno de los eventos mas importantes en el proceso de maduracion de los gametos masculinos en la cabra Black Bengal.

Autologous bone marrow‐derived cells with placental extract for healing excisional cutaneous wounds in animal model

Topical wound‐healing potential of autologous bone marrow‐derived nucleated cells along with placental extract was evaluated in comparison with buffy coat of autologous blood on full‐thickness cutaneous wounds in the thoracolumbar region of 15 clinically healthy New Zealand rabbits. Three wounds of 2 × 2 cm, one on the right side of the body and two on the left side of the midline were created on the dorsal lumbar region of each rabbit under xylazine–ketamine anaesthesia. The wounds of each animal were randomly assigned to one of the three treatments: topical application of autologous bone marrow‐derived cells with placental extract (group I), application of buffy coat in the autologous plasma with placental extract (group II) and autologous plasma with placental extract as control (group III). Wounds were observed for 30 days macroscopically and for granulation tissue formation, histomorphological and histochemical evaluation. Time of appearance of granulation tissues and filling of wound beds were faster in group I followed by group II and group III animals, respectively. Histomorphological findings exhibited an earlier disappearance of inflammatory reaction, better epithelialisation, significantly maximum neovascularisation, fibroplasias and collagenation in group I followed by group II and group III animals, respectively. Histochemical findings also depicted maximum number of robust, thick, interwoven type of collagen fibres, stout, highly tortuous and interwoven network of elastin fibres and numerous mesh war form of reticulin fibres within the dermal component were present in group I when compared with group II and III animals. Experiment conclude that single application of autologous bone marrow‐nucleated cells with placental extract topically could be a novel option for faster healing in complicated non healing wounds both in human beings and animals.

Sperm Morphology and Natural Biomolecules from Marine Snail Telescopium telescopium: a Phylogenetic Perspective

El analisis bioquimico de la fraccion aislada del citosol desde las glandulas ovotestes/espermateca del molusco marino Telescopium telescopium y su estructura tubular espermatica revelaron biomoleculas relativamente similares como tales como diferentes enzimas, hormonas, minerales y estructuras de los espermatozoides que tambien existen en los seres humanos. Por otra parte, en el antisuero de la fraccion citosolica se encontro una reaccion cruzada con los antigenos del esperma humano indicando la presencia de una superficie espermatica de antigenicidad comun entre estas dos diversificadas especies. Estos hallazgos pueden apoyar y/o hipotetizar sobre el origen y la diversificacion de las moleculas de los vertebrados desde su forma (s) ancestral desde los invertebrados, y funciones basicas fisiologicas de estas biomoleculas ancestrales incluyendo algunas de las estructuras celulares siendo plausiblemente las mismas, independientemente de sus cambios estructurales incluso despues de la evolucion.

Is lithium essential for epididymal sperm maturation

A wider biological role of ultratrace element lithium in the mammalian reproduction has been reported, however, presence of lithium in the epididymal luminal fluid (ELF) and its influence on sperm during maturation events in the epididymal regions are still unknown. A pilot study was carried out in Jamunapari buck which revealed that levels of lithium in the ELF diminished gradually and significantly (P<0.01) from caput to cauda epididymis, concomitantly, a distinct increase (P<0.01) in the spermatozoan motility, viability and hypo-osmotic reactive sperm were observed, except spermatozoan motility that was found absent in the caput epididymis. Therefore, we hypothesize that levels of lithium in the epididymal regions is one of the motility initiation and/or regulatory factor for epididymal sperm maturation essential for acquiring fertilizing competence of sperm cells, hence, lithium could also be considered as one of the biomarker of sperm maturation in any species.

Structural Modification of the Black Bengal Buck (Capra hircus) Acrosome During Post-testicular Maturation of Spermatozoa

El presente estudio se realizo para medir diversos parametros biometricos del acrosoma (AC) intacto/normal recogido desde la cabeza, cuerpo y cola del epididimo y vas deferens de la Cabra Black Bengal. Los AC tenidos con Giemsa fueron medidos despues de la captura con camara lucida. Las observaciones revelaron caracteres dimensionales del capuchon acrosomal que disminuyeron gradualmente y de manera significativa (p <0,01, p <0,05) durante fases de maduracion espermatatica en las diferentes regiones del conducto. La forma y tamano del AC tambien fueron influenciados de manera significativa (p <0,01, p <0,05) por la edad y el peso corporal de los animales. La modificacion estructural junto con los cambios morfologicos del AC refleja uno de los indices de maduracion de los gametos masculinos de la Cabra Black Bengal.