Uzma Yunus

Synthesis, characterization and in vitro evaluation of methotrexate conjugated fluorescent carbon nanoparticles as drug delivery system for human lung cancer targeting.

Nanotechnology based cancer therapeutics have rapidly advanced towards the solution of many limitations associated with other drug delivery agents such as nonspecific distribution within the body, low water solubility and non-biocompatibility. Carbon nanoparticles have demonstrated unique properties that are useful to combat with these issues, including their properties dependent on size, high stability in different solvents, compatible size for drug delivery and ease of surface modifications. Fluorescent carbon nanoparticles with good water solubility were obtained from a carbohydrate source by acid assisted ultrasonic treatment at 35kHz for 4h. This simple and economical method can be used for large scale production. Electron microscopic, spectroscopic and thermo gravimetric analysis techniques were used to characterize these carbon nanoparticles. Functionalized CNPs were further conjugated with anticancer drug-methotrexate and used as fluorescent nano-carriers. In this research work, we determined the in vitro bioactivity of CNPs-methotrexate conjugates by lactate dehydrogenase assay, cell adhesion assay and sulforhodamine B assay in human lung carcinoma cell line (H157). The CNPs showed promising biocompatibility and CNPs-MTX conjugates demonstrated potent cytotoxic effects and high anticancer activities in human lung cancer cell line.

Ethyl 4-(3-benzoyl-thio-ureido)benzoate.

The title compound, C17H16N2O3S, crystallizes in the thioamide form with an intramolecular N—H⋯O hydrogen bond across the thiourea system. Molecules are connected in chains parallel to [10] by hydrogen bonds from the second thiourea N—H group to the benzoate C=O function.

Synthesis, Characterization, and Biological Activity of Novel Schiff and Mannich Bases of 4-Amino-3-(N-phthalimidomethyl)-1,2,4-triazole-5-thione

The present work describes the syntheses and antimicrobial activity studies of a series of novel Schiff bases (4a–4i) and their Mannich bases (5a–5h) starting from 4-amino-3-(N-phthalimido-methyl)-1,2,4-triazole-5-thione (3). All the synthesized compounds were characterized by IR, 1H-NMR, 13C-NMR, and MS. All the synthesized compounds were screened for four Gram-negative strains, one Gram-positive strain of bacteria, and one diploid fungal strain. In general the antimicrobial activity increased remarkably on the introduction of azomethine functionality in parent triazole (3). The antimicrobial activity further improved when morpholine group was added to them except for Enterobacter cloacae, where loss of activity was observed. The results are promising and show that the fine tuning of the structures (5a, 5b, 5e, 5f, and 5h) can lead to some new antimicrobial compounds.

1-(1,3-Benzothia­zol-2-yl)-3-benzoyl­thio­urea

The title compound, C15H11N3OS2, was synthesized from benzoyl thiocyanate and 2-aminobenzothiazole in dry acetone. The thiourea group is in the thioamide form. The molecules are stabilized by two intermolecular C—H⋯S and C—H⋯O hydrogen bonds. Intramolecular N—H⋯O hydrogen bonding results in a pseudo-S(6) planar ring with dihedral angles of 11.23 and 11.91° with the benzothiazole ring system and the phenyl ring, respectively.

2-(4-Amino-5-thioxo-4,5-dihydro-1H-1,2,4-triazol-3-ylmeth- yl)isoindoline-1,3-dione

The title compound, C11H9N5O2S, was synthesized from N-phthaloylglycine and thiocarbohydrazide by the fusion method. This is the first report of a triazole derivative of N-phthaloylglycine. The title compound exists in the thione form. The molecule is non-planar, with a dihedral angle between the isoindoline ring system and the triazole ring system of 82.24 (5)°. The crystal structure is stabilized by intermolecular hydrogen bonding linking the molecules into a three-dimensional network.

1-(4-Acetyl-phen-yl)-3-butyrylthio-urea.

The title compound, C13H16N2O2S, crystallizes in the thioamide form with an intramolecular hydrogen bond of type N—H⋯Obutyryl. Molecules are linked into chains parallel to [10] by a further hydrogen bond of type N—H⋯Oacetyl. C—H⋯O and C—H⋯S hydrogen bonds are also present.

2-Propyl-4H-thia-zolo[3,2-a][1,3,5]triazine-4-thione.

In the title compound, C8H9N3S2, the n-propyl chain is disordered over two orientations (site-occupancy ratio = 0.522:0.478) and is roughly perpendicular to the fused thiazolotriazine system. The angle between the fused ring and the propyl chain is 83.6 (1)° [ 82.2 (1)° for the disordered chain]. The structure is stabilized by C—H⋯N hydrogen bonds.

catena-Poly[[tri-phenyl-tin(IV)]-μ-N-(4-acetyl-phen-yl)maleamato].

The crystal structure of the polymeric title compound, [Sn(C6H5)3(C12H10NO4)]n, comprises polymeric chains whereby adjacent Sn atoms are bridged by carboxylate and amide carbonyl O atoms [Sn—O = 2.115 (15) and 2.653 (1) Å, respectively]. The SnIV atom is five-coordinated showing a distorted trigonal–bipyramid geometry, with the three phenyl ipso-C atoms defining the trigonal plane and the axial positions occupied by O atoms [O—Sn—O = 171.91 (5)°]. Intramolecular N—H⋯O hydrogen bonding leads to a seven-membered loop. There is an intramolecular C—H⋯O interaction within the polymeric chain. An intermolecular C—H⋯O interaction along c links the polymeric chains into sheets which are linked into a three-dimensional network via C—H⋯π interactions.

Caffeine–N-phthaloyl-β-alanine (1/1)

The title co-crystal [systematic name: 3-(1,3-dioxoisoindolin-2-yl)propanoic acid–1,3,7-trimethyl-1H-purine-2,6(3H,7H)-dione (1/1)], C8H10N4O2·C11H9NO4, is the combination of 1:1 adduct of N-phthaloyl-β-alanine with caffeine. The phthalimide and purine rings in the N-phthaloyl-β-alanine and caffeine molecules are essentially planar, with r.m.s. deviations of the fitted atoms of 0.0078 and 0.0118 Å, respectively. In the crystal, the two molecules are linked via an O—H⋯N hydrogen bond involving the intact carboxylic acid (COOH) group. The crystal structure is consolidated by C—H⋯O interactions. The H atoms of a methyl group of the caffeine molecule are disordered over two sets of sites of equal occupancy.

The 1:1 adduct of caffeine and 2-(1,3-dioxoisoindolin-2-yl)acetic acid

In the crystal structure of the title adduct [systematic name: 2-(1,3-dioxoisoindolin-2-yl)acetic acid–1,3,7-trimethyl-1,2,3,6-tetrahydro-7H-purine-2,6-dione (1/1)], C8H10N4O2·C10H7NO4, the components are linked by an O—H⋯N hydrogen-bond and no proton transfer occurs.