V. F. Kobzev
Russian Academy of Sciences
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FEBS Letters | 1999
Gennady V. Vasiliev; Vasily M. Merkulov; V. F. Kobzev; T. I. Merkulova; M. P. Ponomarenko; N. A. Kolchanov
Single base mutations G→A at position 663 and G→T at position 666 of intron 6 of the human tryptophan oxygenase gene (TDO2) are associated with a variety of psychiatric disorders [Comings, D.E. et al. (1996) Pharmacogenetics 6, 307–318]. Binding of rat liver nuclear extract proteins to synthetic double‐strand oligonucleotides corresponding to three allelic states of the region between 651 bp and 680 bp of human TDO2 intron 6 has been studied by gel shift assay. It has been demonstrated that to each allelic state of the region there corresponds a specific set of proteins that interacts with it. With the aid of computer analysis and using specific anti‐YY‐1 antibodies it has been shown that both mutations damage the YY‐1 transcription factor binding site.
Human Genetics | 1998
N. S. Yudin; V. Vinogradov Sergey; Tatyana A. Potapova; Tatyana M. Naykova; Violetta V. Sitnikova; Igor V. Kulikov; Viacheslav I. Khasnulin; Cheche Konchuk; Pavel E. Vloschinskii; Sergey V. Ivanov; V. F. Kobzev; Aida G. Romaschenko; M. I. Voevoda
Abstract 32-bp inactivating deletion in the β-chemokine receptor 5 (CCR5) gene, common in Nothern European populations, is associated with reduced HIV-1 transmission risk and delayed disease progression. We have studied the deletion distribution in many populations in Eurasia by polymerase chain reaction analysis of 531 DNA samples representing West and East Siberian, Central Asian, and Far Eastern parts of Russia. An unusually high frequency (11.1%) of the deleted variant in natives of West Siberia, of Finno-Ugrian descent, was observed. Furthermore, the deletion was infrequent in indigenous populations of Central Asia, East Siberia, the Russian Far East, and Canada. We conclude that the Δccr5 distribution is limited primarily to Europeans and related western Siberian Finno-Ugrian populations, with a sharp negative gradient toward the east along the territory of Russian Asia.
Russian Journal of Genetics | 2003
S. V. Mikhailova; V. F. Kobzev; I. V. Kulikov; Aida G. Romaschenko; V. I. Khasnulin; M. I. Voevoda
The incidence of hereditary hemochromatosis as well as the predisposition to the iron overload syndrome and sporadic porphyria cutanea tarda are currently believed to be associated with the inheritance of certain allelic variants of the HFE gene. Allele frequencies of the C282Y (845A) and H63D(187G) mutations in theHFE gene in human populations of different races are remarkably different, and the prevalence of the S65C (193T) mutation is still poorly studied. In the present study we estimated allele frequencies ofHFE mutations in Russians and in a number of Siberian ethnic indigenous populations. In Russians, the allele frequencies of the C282Y, H63D andS65C mutations were 3.7, 13.3 and 1.7%, respectively. These values were similar to those observed in populations of Europe. The C282Y mutation was not detected in the population samples of Siberian ethnic groups, including Mansis, Khantys (Finno-Ugric group), Altaians, and Nivkhs (Asians), suggesting that the frequency of this allele in the populations examined was lower than 1%. The frequency of the C282Y allele in the Tuvinian and Chukchi samples (Asians) constituted 0.45 and 0.8%, respectively. Furthermore, pedigree analysis of both identified Chukchi carriers discovered showed that some of their ancestors were from other ethnic groups. Low frequencies of this allelic variant were typical of many Eastern Asian populations, which were also characterized by rather low frequencies of the H63D variant. In contrast, in some ethnic groups of Western Siberia, the allelic frequency of the H63D mutation was rather high, constituting 8.5% in Altaians, 15.5% in Mansis, and 11.3% in Khantys. The frequency of this allele in Tuvinians, Nivkhs, and Chukchis constituted 5.0, 4.7, and 0.8%, respectively. These findings made it possible to estimate the proportion of individuals predisposed to the iron overload syndrome in different Russian ethnic groups. TheHFE allele frequency distribution patterns observed in the populations examined pointed to pre-Celtic appearance of the C282Y allele. It also provides an explanation of the evolutionary genetic relationships between Siberian ethnic groups and the contemporary populations of Eastern and Western Europe.
Russian Journal of Genetics | 2008
S. V. Nikitin; N. S. Yudin; S. P. Knyazev; R. B. Aytnazarov; V. F. Kobzev; V. A. Bekenev; M. A. Savina; V. I. Yermolaev
Statistical analysis of the frequency of chromosomes carrying three types (A, B, and C) of porcine endogenous retroviruses (PERV) was based on molecular-genetic testing of populations of domestic pigs and wild boars. Domestic pigs were shown to have higher frequency of PERV and haplotypes containing two and three types of PERV than wild boars.
Molecular Biology | 2010
Andrey V. Barkhash; V. N. Babenko; V. F. Kobzev; Aida G. Romaschenko; M. I. Voevoda
Abstract2′-5′-oligoadenylate synthetases are a family of interferon-induced enzymes playing an important role in antiviral defense in mammals. In the human genome, three genes encoding functional synthetases (OAS1, OAS2 and OAS3) form a cluster. Previously, we found that particular genotypes and/or alleles of five single nucleotide polymorphisms (SNPs) of OAS2 and OAS3 are associated with predisposition to severe forms of tick-borne encephalitis (TBE) in Russians. In the current study, we investigated the distribution of three of the above SNPs, OAS3 rs2285932 (C/T, Ile438Ile), OAS3 rs2072136 (G/A, Ser567Ser), and OAS2 rs15895 (G/A, Trp720Ter relative to p71 isoform), in seven populations of North Eurasia: Caucasians (Russians, Germans from Altai region), Central Asian Mongoloids (Altaians, Khakass, Tuvinians, and Shorians), and Arctic Mongoloids (Chukchi). Interpopulational differences in genotype, allele and haplotype frequencies and in linkage disequilibrium structure for these SNPs were detected. These frequencies correlated with the ethnicity of the populations and with their supposed differential exposure to the TBE virus. In particular, the lowest frequencies of G/G genotype for OAS3 SNP rs2072136 (which, according to our earlier results, is associated with predisposition to severe forms of TBE) were found in Altaians, Khakass, Tuvinians, and Shorians, who commonly contact with the TBE virus in their habitation regions. Thus, the data obtained suggest that the TBE virus might act as a selection factor for particular OAS variants in Central Asian Mongoloids.
Bulletin of Experimental Biology and Medicine | 2014
Tatyana A. Potapova; V. N. Babenko; V. F. Kobzev; A. G. Romashchenko; V. N. Maksimov; M. I. Voevoda
We analyzed associations of single nucleotide polymorphisms rsl13004520 (R247T), rs11562975 (L250L), rs7593557 (S419N), rs11563208 (I1016I), and rs11563071 (V1058V) of the cold receptor TRPM8 (2q37.1) gene with blood plasma lipids and anthropometric parameters in Russian population (randomly chosen residents of Novosibirsk: 507 women and 459 men, mean age 57 years). The studied polymorphisms are localized in regions encoding NH2-terminal (R247T, L250L, S419N) and COOH-terminal (I1016I, V1058V) cytoplasmic domains of the channel. We showed association of single nucleotide polymorphism V1058V with the levels of total cholesterol and LDL and HDL cholesterol, and association of I1016I polymorphism with triglyceride content. Polymorphisms L250L and S419N correlated with anthropometric parameters (body mass index and waist and hip circumferences).
Russian Journal of Genetics | 2011
N. S. Yudin; M. V. Nefedova; V. F. Kobzev; Aida G. Romaschenko; M. I. Voevoda
The region of intron 2 of the SDF1 gene encoding a chemokine of the CXC subfamily has been resequenced in Galloway, Hereford, and Black Pied cattle. Five of the single-nucleotide polymorphisms (SNP) that were earlier detected by other authors in various breeds of cattle in North America (99C/G, 128T/C, 206C/T, 267C/G and 313C/T) have been found. The 270insC polymorphic marker has proved to be monomorphic in Russian cattle breeds. Hereford cattle significantly differ from Galloway and Black Pied cattle in the frequencies of some SNP variants and their combinations. The number of SNP combinations in Hereford and Galloway cattle exceeds that in Black Pied cattle.
Russian Journal of Genetics | 2004
T. N. Kouznetsova; M. I. Voevoda; O. A. Podkolodnaya; I. V. Kulikov; V. F. Kobzev; S. N. Ustinov; S. K. Maliutina; N. I. Logvinenko; N. V. Cherdinzeva; Yu. V. Tumanov; O. A. Morozova; V. A. Baum; Aida G. Romaschenko
Analysis of deletion polymorphism of the humanc-fms gene intron 11 (∼425-bp deletion) is of particular interest because of the increased proportion of the heterozygotes among the children born from parents, one of which lacks the deletion-carrying allele, and the other is heterozygous for this allele. In this study, allele and haplotype frequencies of the polymorphism were assessed in a number of Caucasoid and Mongoloid populations of Russia. In all populations tested, relatively high prevalence of the deletion-bearing allele, ranging from 9.45% in ethnic Germans to 20.75% in Altaians, was detected. Russians and Kazakhs were characterized by intermediate frequencies of the rare allele, constituting in these populations 12.89 and 14.93%, respectively. Hardy–Weinberg expectations were met in all populations examined, pointing to a stable level of polymorphism at the c-fms intron 11. It was established by the contextual analysis of the deleted DNA fragment along with the flanking sequences that this region contained a number of transcription factor motifs (ets, CArG, and myc), potentially capable of the regulation of the M-CSF-dependant c-fms transcription. The deletion breakpoint was localized within the CArG motif, which, together with the neighboring ets motif, form the potential CArG/ets composite element. It was suggested that the allele lacking the fragment of intron 11 might be restricted in its ability to modulate the level of the c-fms transcription in response to M-CSF. The molecular epidemiological survey provided indirect evidence favoring the suggestion on the possible functional value of this gene fragment. It was demonstrated that in the samples from acute bronchitis and trichomoniasis patients allelic and genotype frequencies were significantly different from those in the population sample. In case of trichmoniasis, the frequency of rare allele was 2.4 times lower, and in case of acute bronchitis it was 2.1 times higher than in the control sample.
Archaeological and Anthropological Sciences | 2010
Aleksandr S. Pilipenko; Aida G. Romaschenko; V.I. Molodin; Hermann Parzinger; V. F. Kobzev
Archaeology, Ethnology and Anthropology of Eurasia | 2008
Aleksandr S. Pilipenko; Aida G. Romaschenko; V.I. Molodin; I.V. Kulikov; V. F. Kobzev; D.V. Pozdnyakov; O.I. Novikova