V. Karthick

Facile green synthesis of gold nanoparticles using leaf extract of antidiabetic potent Cassia auriculata

A simple biological method for the synthesis of gold nanoparticles (AuNPs) using Cassia auriculata aqueous leaf extract has been carried out in the present study. The reduction of auric chloride led to the formation of AuNPs within 10 min at room temperature (28°C), suggesting a higher reaction rate than chemical methods involved in the synthesis. The size, shape and elemental analysis were carried out using X-ray diffraction, TEM, SEM-EDAX, FT-IR and visible absorption spectroscopy. Stable, triangular and spherical crystalline AuNPs with well-defined dimensions of average size of 15-25 nm were synthesized using C. auriculata. Effect of pH was also studied to check the stability of AuNPs. The main aim of the investigation is to synthesize AuNPs using antidiabetic potent medicinal plant. The stabilizing and reducing molecules of nanoparticles may promote anti-hyperglycemic if tested further.

Sargassum myriocystum mediated biosynthesis of gold nanoparticles.

Functionalized metal nanoparticles are unique in nature and are being developed for its specificity in drug targeting. In the present study, aqueous extract of Sargassum myriocystum is used for the biosynthesis of gold nanoparticles (AuNPs) by the reduction of chloroauric acid. The formation of nanoparticles reaction was complete within 15 min at 76 °C. The size, shape and elemental analysis of AuNPs were carried out using UV-visible absorption spectroscopy, FT-IR, TEM, SEM-EDAX, and XRD analysis. The newly formed AuNPs are stable, well-defined, polydispersed (triangular and spherical) and crystalline with an average size of 15 nm. The biomolecule involved in stabilizing AuNPs was identified using GC-MS.

Facile synthesis of silver chloride nanoparticles using marine alga and its antibacterial efficacy

Exploitation of advancements in antimicrobial agent synthesis assisted by nanomaterials has received considerable attention in the recent years. Based on this, an eco-friendly approach for the synthesis of silver chloride nanoparticles (AgClNPs) using aqueous extract of Sargassum plagiophyllum is emphasized. UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), high resolution transmission electron microscopy (HR-TEM), field emission scanning electron microscopy (FESEM) were used to characterize the formation of AgClNPs. X-ray diffraction (XRD) patterns clearly illustrate the presence of AgClNPs. The synthesized AgClNPs were tested for its antibacterial activity and it was found to cause considerable amount of deterioration to bacterial cells, when examined using electron microscope and cell viability analysis.

Blue green alga mediated synthesis of gold nanoparticles and its antibacterial efficacy against Gram positive organisms.

Biofunctionalized gold nanoparticles (AuNPs) play an important role in design and development of nanomedicine. Synthesis of AuNPs from biogenic materials is environmentally benign and possesses high bacterial inhibition and bactericidal properties. In the present study, blue green alga Spirulina platensis protein mediated synthesis of AuNPs and its antibacterial activity against Gram positive bacteria is discussed. AuNPs were characterized using Ultraviolet-visible (UV-vis) spectroscopy, Fluorescence spectroscopy, Fourier Transform-Infrared (FTIR) spectroscopy, Raman spectroscopy, High Resolution-Transmission Electron Microscopy (HR-TEM) and Energy Dispersive X-ray analysis (EDAX). Stable, well defined AuNPs of smaller and uniform shape with an average size of ~ 5 nm were obtained. The antibacterial efficacy of protein functionalized AuNPs were tested against Gram positive organisms Bacillus subtilis and Staphylococcus aureus.

Effect of biologically synthesized gold nanoparticles on alloxan-induced diabetic rats-an in vivo approach.

Development of novel antidiabetic agents using various organic compounds and biomolecules has been in practice for a long time. Recently, nanomaterials are also being used in antidiabetic studies for their unique properties such as small size, biocompatibility and ability to penetrate cell membrane for carrying drugs. Herein, in vivo antidiabetic activity of gold nanoparticles (AuNPs) synthesized using the antidiabetic potent plant Gymnema sylvestre R. Br on wistar albino rats has been evaluated. The formation of AuNPs and their morphology were confirmed using spectroscopic and microscopic analyses, respectively. The treatment of AuNPs has shown significant reduction in blood glucose level on diabetic rats. AuNPs were also tested for its anti-inflammatory effect by estimating the serum levels of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and high-sensitive C-reactive protein (CRP).

Size controlled biogenic silver nanoparticles as antibacterial agent against isolates from HIV infected patients

Silver nanoparticles (AgNPs) are synthesized using biological sources due to its high specificity in biomedical applications. Herein, we report the size and shape controlled synthesis of AgNPs using the aqueous extract of blue green alga, Spirulina platensis. Size, shape and elemental composition of AgNPs were characterized using UV-vis spectroscopy, Fluorescence spectroscopy, FT-IR (Fourier Transform-Infrared Spectroscopy), FT-RS (Fourier Transform-Raman Spectroscopy), SEM-EDAX (Scanning Electron Microscopy-Energy Dispersive X-ray analysis) and HR-TEM (High Resolution Transmission Electron Microscopy). AgNPs were stable, well defined and monodispersed (spherical) with an average size of 6 nm. The synthesized AgNPs were tested for its antibacterial potency against isolates obtained from HIV patients.

Pectin mediated gold nanoparticles induces apoptosis in mammary adenocarcinoma cell lines

Pectin and its several modified forms have shown remarkable impact in therapeutic use against various cancers. In the present study, pectin, an anionic polysaccharide isolated from Musa paradisiaca is employed for the synthesis of gold nanoparticles at ambient temperature conditions. The synthesized nanoparticles were characterized using microscopic and spectroscopic studies and its anti-cancer potential was evaluated in mammary adenocarcinoma cell lines MCF-7 and MDA-MB-231. Apoptosis induction was evident from increase in sub-G1 population studied using flow cytometry analysis. DNA damage followed by cell death in pectin mediated gold nanoparticles (p-GNPs) treated cells was confirmed by Comet assay. Uptake of p-GNPs by cancer cells (MCF-7 and MDA-MB-231) was analyzed using FE-SEM which revealed the presence of p-GNPs as aggregates over the surface of cells with loss in cellular integrity compared to control cells.

Green Synthesis of Well Dispersed Nanoparticles using Leaf Extract of Medicinally useful Adhatoda Vasica Nees

Abstract: Development of reliable method for the green synthesis of gold nanoparticles (AuNPs) using medicinally valued Adhatoda vasica Nees has been studied here. The color change and the Surface Plasmon Resonance (SPR) confirmed the formation of AuNPs. The biosynthesized AuNPs were characterized using UV-visible Spectroscopy (UV-vis), Fourier Transform Infrared Spectroscopy (FT-IR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDAX) and Transmission Electron Microscopy (TEM) analysis. The nanoparticles synthesized were predominantly monodisperse, stable spherical in nature with well-defined dimensions of size ranging from 22 to 47 nm. The crystalline nature of the synthesized particles was also evident by the X-ray diffraction analysis. Keywords: Adhatoda vasica, Gold nanoparticles, Biosynthesis, Electron Microscopy, Diffraction. INTRODUCTION Nanobiotechnology combines biological principles with physical and chemical procedures to generate nano-sized particles with well defined functions. Particles of interestingly small size make the field of drug delivery more interesting and effective. Synthesizing gold particles (AuNPs) with medicinal applications is the recent trend in the field of nanobiotechnology. The surface availability of nanoparticles for binding/reactivity of other species on them is an important function as it is synthesized in different structures like nanorods, spheres, prims and hexagons. The controlled growth of AuNPs of different morphologies and the various chemical mechanisms involved in the anisotropic growth were studied using different chemical procedures [1]. The low toxicity effects of green synthesized AuNPs on biological systems made researchers to synthesize it by biological method rather by chemical means. Extensive studies were done on AuNPs and its binding affinity towards nucleic acids and proteins in biological systems [2]. AuNPs have been synthesized from various sources like plants [3], microbes [4], seaweeds [5] and microalgae [6]”. Sastry

Hydrothermal synthesis of hydroxyapatite plates prepared using low molecular weight heparin (LMWH)

Materials with enhanced physical and biological properties have been used for biomedical applications and can be developed by functionalizing them using various components. Hydroxyapatite (HAP), among other available synthetic material, serves as one of the best tools in orthopaedics and ceramic coatings. The porous structure of HAP helps in bone cell regeneration, chemical integration of bone and also favours the interaction between bone and tissues. Herein, we have demonstrated a simple procedure for the synthesis of HAP using low molecular weight heparin (LMWH), a structural analogue of bone heparan sulphate proteoglycan. The presence of small sized HAP plates with well-defined structures was revealed using electron microscopic analysis. The phase purity of the synthesized HAP was evaluated using X-ray diffraction pattern obtained before and after immersion in simulated body fluid (SBF).

Effect of biosynthesized gold nanoparticles by Sargassum swartzii in alloxan induced diabetic rats

Biosynthesis of gold nanoparticles (AuNPs) using Sargassum swartzii and its anti-diabetic effect were studied using male wistar Albino rats. Formation of AuNPs were confirmed by UV-vis spectroscopy, Fourier transformed infrared (FTIR) spectroscopy, High-Resolution transmission electron microscopy (HR-TEM) and X-ray diffraction (XRD). Fasting blood glucose levels, serum insulin, hemoglobin and glycosylated hemoglobin levels in diabetic treated rats with AuNPs were significantly decreased compared to the control group. The results of the blood glucose level and serum insulin levels indicated that AuNPs could significantly improve the insulin resistance and glucose level in diabetic rats. AuNPs also shows reduction in anti-inflammation, tumor necrosis factor-alpha, interleukin-6 and high-sensitive C-reactive protein in diabetic rats. The data showed that AuNPs synthesized using S. swartzii exerted antidiabetic effect, accordingly improve pancreas, liver and kidney damage caused by alloxan induced diabetic rats.