V. P. Polischuk

Virus Diseases and Crop Biosecurity

Preface. A short History of Warfare Emphasising the biological Aspects D.J. Osborne.- Crop Viruses and Virus Diseases: A Global Perspective J.M. Thresh.- Plant Viruses in European Agriculture: Current Problems and Future Aspects R.A.A. Van der Vlugt.- Significant Ways to spread Plant Virus Diseases in agricultural Ecosystems: Is Agroterrorism possible? G. Adam.- Soil-borne Viruses of Crop Plants - Potential Agents for bioterrorist Attacks? T. Kuhne.- Genomic Approaches in Virus Diagnostics: A personal Assessment of Realities when faced with Viruses in a Plant Biosecurity Context J.I. Cooper.- Molecular Methods for Detection and Quantitation of Viruses in Aphids M. Cambra.- The Use of monoclonal Antibodies in Plant Protection and for Studying Virus-induced pathogenic Processes T. Erokhina.- Identification of Plant Host Factors interacting with Viruses: Novel Targets for Virus Control M. Taliansky.- Current View on Host Components involved in Plant Virus intercellular Trafficking S. Morozov.- Abiotic environmental Factors: Effects on Epidemiology of Plant Virus Infections V. Polischuk.- Somaclonal Variation as a Source of Tomato Spotted Wilt Virus-Resistance in Plants I.S. Shcherbatenko.- List of contributors.- Index.

Investigation of Ukrainian isolates of cucumber green mottle mosaic virus

Abstract Isolates of cucumber green mottle mosaic virus (CGMMV) obtained from three greenhouses in Ukraine have been identified. The rabbit antisera have been generated for these isolates. These antisera can be used for diagnostics of CGMMV in plant material by indirect ELISA. Analysis of cDNA sequences of capsid protein of these viruses confirmed that they may be grouped into novel strain of CGMMV.

Monitoring of some phytoviral infections in the Ukraine

This paper presents the results of 3 year‐long monitoring of crop fields in four ecologically different areas of Ukraine for several phytoviral infections. Various modifications of ELISA were used as main assay to evaluate the spreading of a virus. The technique allowing to determine the frequency of occurrence for a virus of interest is proposed. This technique is based on an identification of viral antigens in crops, accompanying weeds and soil. The differences between the investigated regions on a virus occurrence frequency as well as dynamics of the parameter in relation to crop‐rotation have been revealed. The possibility to use results obtained for predicting phytoviral disease spreading is discussed.

The exploration of rhabdoviruses infecting agricultural plants in conditions of the Ukraine

Bacillus‐like viruses were revealed in plants of sugar beet (300 × 42 ran), winter wheat (245–350 × 40–70 nm), black currant (250–285 × 65–85), raspberry (460–490 × 70–90) in the Ukraine. The viruses’ morphology and their intracellular localization was studied with electron microscopy.

POTYVIRUSES INFECTING VEGETABLE CROPS IN UKRAINE

This paper describes detection of some potyvirus infecting vegetable crops in Ukraine. Collected samples were screened for the presence of Zucchini yellow mosaic virus and Watermelon mosaic virus-2. Obtained isolates of Zucchini yellow mosaic virus were clustered with isolates from Slovenia, Hungary, Czech Republic, Austria and France within subgroup AI. According to the topology of Neighbor-Joining tree based on sequences of NIb-CP genome region obtained WMV-2 isolates showed that belong to group G1. Viruses infecting cucurbits in Ukraine presented by phylogenetic groups widespread in Europe.