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Featured researches published by V.V. Pully.


PLOS ONE | 2012

Replacement of Retinyl Esters by Polyunsaturated Triacylglycerol Species in Lipid Droplets of Hepatic Stellate Cells during Activation

Nicole Testerink; Mokrish Ajat; Martin Houweling; Jos F. Brouwers; V.V. Pully; Henk-Jan van Manen; Cees Otto; J. Bernd Helms; Arie B. Vaandrager

Activation of hepatic stellate cells has been recognized as one of the first steps in liver injury and repair. During activation, hepatic stellate cells transform into myofibroblasts with concomitant loss of their lipid droplets (LDs) and production of excessive extracellular matrix. Here we aimed to obtain more insight in the dynamics and mechanism of LD loss. We have investigated the LD degradation processes in rat hepatic stellate cells in vitro with a combined approach of confocal Raman microspectroscopy and mass spectrometric analysis of lipids (lipidomics). Upon activation of the hepatic stellate cells, LDs reduce in size, but increase in number during the first 7 days, but the total volume of neutral lipids did not decrease. The LDs also migrate to cellular extensions in the first 7 days, before they disappear. In individual hepatic stellate cells. all LDs have a similar Raman spectrum, suggesting a similar lipid profile. However, Raman studies also showed that the retinyl esters are degraded more rapidly than the triacylglycerols upon activation. Lipidomic analyses confirmed that after 7 days in culture hepatic stellate cells have lost most of their retinyl esters, but not their triacylglycerols and cholesterol esters. Furthermore, we specifically observed a large increase in triacylglycerol-species containing polyunsaturated fatty acids, partly caused by an enhanced incorporation of exogenous arachidonic acid. These results reveal that lipid droplet degradation in activated hepatic stellate cells is a highly dynamic and regulated process. The rapid replacement of retinyl esters by polyunsaturated fatty acids in LDs suggests a role for both lipids or their derivatives like eicosanoids during hepatic stellate cell activation.


Analytical Chemistry | 2010

Microbioreactors for Raman Microscopy of Stromal Cell Differentiation

V.V. Pully; Aufried Lenferink; Henk-Jan van Manen; Vinod Subramaniam; Clemens van Blitterswijk; Cees Otto

We present the development of microbioreactors with a sensitive and accurate optical coupling to a confocal Raman microspectrometer. We show that such devices enable in situ and in vitro investigation of cell cultures for tissue engineering by chemically sensitive Raman spectroscopic imaging techniques. The optical resolution of the Raman microspectrometer allows recognition and chemical analysis of subcellular features. Human bone marrow stromal cells (hBMSCs) have been followed after seeding through a phase of early proliferation until typically 21 days later, well after the cells have differentiated to osteoblasts. Long-term perfusion of cells in the dynamic culture conditions was shown to be compatible with experimental optical demands and off-line optical analysis. We show that Raman optical analysis of cells and cellular differentiation in microbioreactors is feasible down to the level of subcellular organelles during development. We conclude that microbioreactors combined with Raman microspectroscopy are a valuable tool to study hBMSC proliferation, differentiation, and development into tissues under in situ and in vitro conditions.


Biomaterials | 2012

Self-attaching and cell-attracting in-situ forming dextran-tyramine conjugates hydrogels for arthroscopic cartilage repair

Liliana Moreira Teixeira; Suzanne Bijl; V.V. Pully; Cees Otto; Rong Jin; Jan Feijen; Clemens van Blitterswijk; Pieter J. Dijkstra; Marcel Karperien


Journal of Physical Chemistry C | 2010

High-Density Periodic Arrays of Self-Aligned Subwavelength Nanopyramids for Surface-Enhanced Raman Spectroscopy

Mingliang Jin; V.V. Pully; Cees Otto; Albert van den Berg; Edwin T. Carlen


Journal of Raman Spectroscopy | 2011

Time Lapse Raman Imaging of Single Live Lymphocytes

V.V. Pully; Aufrid T.M. Lenferink; Cornelis Otto


Journal of Raman Spectroscopy | 2009

The intensity of the 1602 cm−1 band in human cells is related to mitochondrial activity

V.V. Pully; Cees Otto


Journal of Raman Spectroscopy | 2009

Hybrid Rayleigh, Raman and two‐photon excited fluorescence spectral confocal microscopy of living cells

V.V. Pully; Aufried Lenferink; Cees Otto


Vibrational Spectroscopy | 2010

Raman-fluorescence hybrid microspectroscopy of cell nuclei

V.V. Pully; Aufrid T.M. Lenferink; Cornelis Otto


Archive | 2009

Raman monitoring of chondrocyte dedifferentiation and differentiation

Aliz Kunstar; V.V. Pully; Cornelis Otto; Clemens van Blitterswijk; Aart A. van Apeldoorn


Chemistry and Physics of Lipids | 2009

Raman imaging and lipidomic analysis of lipid droplets in (activated) hepatic stellate cells

Arie B. Vaandrager; Nicole Testerink; Mokrish Ajat; Martin Houweling; Jos F. Brouwers; V.V. Pully; H.J. van Manen; Cornelis Otto; J.B. Helms

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Cornelis Otto

MESA+ Institute for Nanotechnology

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Aufried Lenferink

MESA+ Institute for Nanotechnology

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Albert van den Berg

MESA+ Institute for Nanotechnology

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