Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Valentina Guida is active.

Publication


Featured researches published by Valentina Guida.


American Journal of Human Genetics | 2000

Tracing European Founder Lineages in the Near Eastern mtDNA Pool

Martin B. Richards; Vincent Macaulay; Eileen Hickey; Emilce Vega; Bryan Sykes; Valentina Guida; Chiara Rengo; Daniele Sellitto; Fulvio Cruciani; Toomas Kivisild; Richard Villems; Mark G. Thomas; Serge Rychkov; Oksana Rychkov; Yuri Rychkov; Mukaddes Gölge; Dimitar Dimitrov; Emmeline W. Hill; Daniel G. Bradley; Valentino Romano; Francesco Calì; Giuseppe Vona; Andrew G. Demaine; S.S. Papiha; Costas Triantaphyllidis; Gheorghe Stefanescu; Jiři Hatina; Michele Belledi; Anna Di Rienzo; Andrea Novelletto

Founder analysis is a method for analysis of nonrecombining DNA sequence data, with the aim of identification and dating of migrations into new territory. The method picks out founder sequence types in potential source populations and dates lineage clusters deriving from them in the settlement zone of interest. Here, using mtDNA, we apply the approach to the colonization of Europe, to estimate the proportion of modern lineages whose ancestors arrived during each major phase of settlement. To estimate the Palaeolithic and Neolithic contributions to European mtDNA diversity more accurately than was previously achievable, we have now extended the Near Eastern, European, and northern-Caucasus databases to 1,234, 2, 804, and 208 samples, respectively. Both back-migration into the source population and recurrent mutation in the source and derived populations represent major obstacles to this approach. We have developed phylogenetic criteria to take account of both these factors, and we suggest a way to account for multiple dispersals of common sequence types. We conclude that (i) there has been substantial back-migration into the Near East, (ii) the majority of extant mtDNA lineages entered Europe in several waves during the Upper Palaeolithic, (iii) there was a founder effect or bottleneck associated with the Last Glacial Maximum, 20,000 years ago, from which derives the largest fraction of surviving lineages, and (iv) the immigrant Neolithic component is likely to comprise less than one-quarter of the mtDNA pool of modern Europeans.


American Journal of Human Genetics | 1999

The emerging tree of West Eurasian mtDNAs: a synthesis of control-region sequences and RFLPs.

Vincent Macaulay; Martin B. Richards; Eileen Hickey; Emilce Vega; Fulvio Cruciani; Valentina Guida; Rosaria Scozzari; Batsheva Bonne-Tamir; Bryan Sykes; Antonio Torroni

Variation in the human mitochondrial genome (mtDNA) is now routinely described and used to infer the histories of peoples, by means of one of two procedures, namely, the assaying of RFLPs throughout the genome and the sequencing of parts of the control region (CR). Using 95 samples from the Near East and northwest Caucasus, we present an analysis based on both systems, demonstrate their concordance, and, using additional available information, present the most refined phylogeny to date of west Eurasian mtDNA. We describe and apply a nomenclature for mtDNA clusters. Hypervariable nucleotides are identified, and the relative mutation rates of the two systems are evaluated. We point out where ambiguities remain. The identification of signature mutations for each cluster leads us to apply a hierarchical scheme for determining the cluster composition of a sample of Berber speakers, previously analyzed only for CR variation. We show that the main indigenous North African cluster is a sister group to the most ancient cluster of European mtDNAs, from which it diverged approximately 50,000 years ago.


American Journal of Human Genetics | 2004

Phylogeographic analysis of haplogroup E3b (E-M215) y chromosomes reveals multiple migratory events within and out of Africa.

Fulvio Cruciani; Roberta La Fratta; Piero Santolamazza; Daniele Sellitto; Roberto Pascone; Pedro Moral; Elizabeth Watson; Valentina Guida; Eliane Beraud Colomb; Boriana Zaharova; João Lavinha; Giuseppe Vona; Rashid Aman; Francesco Calì; Nejat Akar; Martin B. Richards; Antonio Torroni; Andrea Novelletto; Rosaria Scozzari

We explored the phylogeography of human Y-chromosomal haplogroup E3b by analyzing 3401 individuals from five continents. Our data refine the phylogeny of the entire haplogroup, which appears as a collection of lineages with very different evolutionary histories, and reveal signatures of several distinct processes of migrations and/or recurrent gene flow that occurred in Africa and western Eurasia over the past 25000 years. In Europe, the overall frequency pattern of haplogroup E-M78 does not support the hypothesis of a uniform spread of people from a single parental Near Eastern population. The distribution of E-M81 chromosomes in Africa closely matches the present area of distribution of Berber-speaking populations on the continent, suggesting a close haplogroup-ethnic group parallelism. E-M34 chromosomes were more likely introduced in Ethiopia from the Near East. In conclusion, the present study shows that earlier work based on fewer Y-chromosome markers led to rather simple historical interpretations and highlights the fact that many population-genetic analyses are not robust to a poorly resolved phylogeny.


American Journal of Human Genetics | 2001

Do the four clades of the mtDNA haplogroup L2 evolve at different rates

Antonio Torroni; Chiara Rengo; Valentina Guida; Fulvio Cruciani; Daniele Sellitto; Alfredo Coppa; Fernando Luna Calderón; Barbara Simionati; Giorgio Valle; Martin B. Richards; Vincent Macaulay; Rosaria Scozzari

Forty-seven mtDNAs collected in the Dominican Republic and belonging to the African-specific haplogroup L2 were studied by high-resolution RFLP and control-region sequence analyses. Four sets of diagnostic markers that subdivide L2 into four clades (L2a-L2d) were identified, and a survey of published African data sets appears to indicate that these clades encompass all L2 mtDNAs and harbor very different geographic/ethnic distributions. One mtDNA from each of the four clades was completely sequenced by means of a new sequencing protocol that minimizes time and expense. The phylogeny of the L2 complete sequences showed that the two mtDNAs from L2b and L2d seem disproportionately derived, compared with those from L2a and L2c. This result is not consistent with a simple model of neutral evolution with a uniform molecular clock. The pattern of nonsynonymous versus synonymous substitutions hints at a role for selection in the evolution of human mtDNA. Regardless of whether selection is shaping the evolution of modern human mtDNAs, the population screening of L2 mtDNAs for the mutations identified by our complete sequence study should allow the identification of marker motifs of younger age with more restricted geographic distributions, thus providing new clues about African prehistory and the origin and relationships of African ethnic groups.


Heart | 2010

Familial transposition of the great arteries caused by multiple mutations in laterality genes

Alessandro De Luca; Anna Sarkozy; Federica Consoli; Rosangela Ferese; Valentina Guida; Maria Lisa Dentici; Rita Mingarelli; Emanuele Bellacchio; Giulia Tuo; Giuseppe Limongelli; Maria Cristina Digilio; Bruno Marino; Bruno Dallapiccola

Background The pathogenesis of transposition of the great arteries (TGA) is still largely unknown. In general, TGA is not associated with the more common genetic disorders nor with extracardiac anomalies, whereas it can be found in individuals with lateralisation defects, heterotaxy and asplenia syndrome (right isomerism). Objective To analyse genes previously associated with heterotaxy in order to assess mutations in familial TGA unassociated with other features of laterality defects. Methods Probands of seven families with isolated TGA and a family history of concordant or discordant congenital heart disease were screened for mutations in the ZIC3, ACVR2B, LEFTYA, CFC1, NODAL, FOXH1, GDF1, CRELD1, GATA4 and NKX2.5 genes. Results Mutation analysis allowed the identification of three sequence variations in two out of seven TGA probands. A FOXH1 (Pro21Ser) missense variant was found in a proband who was also heterozogous for an amino acid substitution (Gly17Cys) in the ZIC3 gene. This ZIC3 variant was also found in another family member with a second sequence variation (Val150Ile) in the NKX2.5 gene homeodomain who was affected by multiple ventricular septal defects. A second proband was found to harbour a splice site variant (IVS2-1G→C) in the NODAL gene. Conclusions The present study provides evidence that some cases of familial TGA are caused by mutations in laterality genes and therefore are part of the same disease spectrum of heterotaxy syndrome, and argues for an oligogenic or complex mode of inheritance in these pedigrees.


Diabetes | 2007

A Functional Variant of the Adipocyte Glycerol Channel Aquaporin 7 Gene Is Associated With Obesity and Related Metabolic Abnormalities

Sabrina Prudente; Elisabetta Flex; Eleonora Morini; Federica Turchi; Daria Capponi; Salvatore De Cosmo; Vittorio Tassi; Valentina Guida; Angelo Avogaro; Franco Folli; Francesca Maiani; Lucia Frittitta; Bruno Dallapiccola; Vincenzo Trischitta

Aquaporin 7 (AQP7), the gateway protein controlling glycerol release, has recently emerged as a modulator of adipocyte metabolism. AQP7 knockout mice develop obesity and hyperglycemia. The contribution of AQP7 to these abnormalities in humans is unknown. We examined whether common single nucleotide polymorphisms (SNPs) in the AQP7 gene modulate the risk of obesity and related abnormalities. Among several SNPs we identified, A-953G in the AQP7 promoter was associated with type 2 diabetes in 977 (530 female/447 male) Caucasians: odds ratio for XG (i.e., AG+GG) versus AA individuals was 1.36 (95% CI 1.01–1.84), P = 0.04. This finding was entirely due to the association among females (1.8 [1.2–2.6], P = 0.004), which was no longer significant when adjusted for BMI. In fact, BMI was higher in XG than in AA females (30.8 ± 6.6 vs. 28.9 ± 5.2, P = 0.002). This association was confirmed in independent case-control study (n = 299 female subjects) for morbid obesity (1.66 [1.01–2.74], P = 0.04). Luciferase and mobility shift assays showed that, compared with −953A, the −953G promoter had reduced transcriptional activity (P = 0.001) and impaired ability to bind CCAAT/enhancer binding protein (C/EBP)β transcription factor (P = 0.01). Finally, AQP7 expression in adipose tissue decreased from AA to AG to GG individuals (P = 0.036). These data strongly suggest that AQP7 downregulation is pathogenic for obesity and/or type 2 diabetes.


European Journal of Human Genetics | 2005

Screening of mutations in the CFTR gene in 1195 couples entering assisted reproduction technique programs

Liborio Stuppia; Ivana Antonucci; Francesco Binni; Alessandra Brandi; Nicoletta Grifone; Alessia Colosimo; Mariella De Santo; Valentina Gatta; Gianfranco Gelli; Valentina Guida; Silvia Majore; Giuseppe Calabrese; Chiara Palka; Anna Ravani; Rosanna Rinaldi; Gian Mario Tiboni; Enzo Ballone; Anna Venturoli; Alessandra Ferlini; Isabella Torrente; Paola Grammatico; Elisa Calzolari; Bruno Dallapiccola

Genetic testing of the cystic fibrosis transmembrane conductance (CFTR) gene is currently performed in couples undergoing assisted reproduction techniques (ART), because of the high prevalence of healthy carriers in the population and the pathogenic relationship with congenital bilateral absence of vas deferens (CBAVD). However, discordant data have been reported concerning the usefulness of this genetic test in couples with no family history of cystic fibrosis (CF). In this study, we report the results of CFTR molecular screening in 1195 couples entering ART. Genetic testing was initially carried out in a single partner of each couple. CFTR mutations were detected in 55 subjects (4.6%), a percentage that overlaps with the one reported in the general population. However, significantly higher frequencies of were found in CBAVD individuals (37.5%) and in males with nonobstructive azoospermia (6.6%). The 5T allele was found in 78 patients (6.5%). This figure was again significantly different in males with nonobstructive-azoospermia (9.9%) and in those with CBAVD (100%). All together, 139 subjects (11.6%) had either a CFTR mutation or the 5T allele. Subsequent molecular analysis of their partners disclosed a CFTR mutation or 5T allele in nine cases (6.5%). However, none of these couples had CFTR alterations in both members, a CFTR mutation being invariably present in one partner and the 5T allele in the other. In order to improve genetic counselling of these couples, the TG-M470V-5T association was analyzed, and a statistically significant relationship between 12TG-V470 and CBAVD was detected.


Blood Cells Molecules and Diseases | 2011

Application of MLPA assay to characterize unsolved α-globin gene rearrangements

Alessia Colosimo; Valentina Gatta; Valentina Guida; Eleonora Leodori; Enrica Foglietta; Silvana Rinaldi; Maria Pia Cappabianca; Antonio Amato; Liborio Stuppia; Bruno Dallapiccola

α-thalassemia belongs to those inherited diseases in which large genomic deletions/duplications represent a significant proportion of causative mutations. Until recently, large α-globin gene cluster rearrangements have been mainly detected by gap-PCR and Southern blotting, methods that have significant drawbacks. We tested the recently developed multiplex ligation-dependent probe amplification (MLPA) assay for deletional screening of the α-globin gene cluster in a cohort of 25 individuals suspected of having α-globin alteration(s), in which no or doubtful mutations had been found using conventional methods. In 13 out of 18 α-thalassemia carriers and in all 5 patients with HbH we found the causative α-globin defects. In 2 thalassemia intermedia patients, carriers of heterozygous β-globin mutations, the co-inheritance of homozygous α-genes triplication was detected. MLPA results were subsequently confirmed by real-time PCR. This study shows that MLPA can effectively identify different and unknown types of α-globin gene rearrangements, to allow characterizing previously unsolved α-thalassemia genotypes.


Journal of Materials Science: Materials in Medicine | 2010

Design of novel three-phase PCL/TZ-HA biomaterials for use in bone regeneration applications

Aurelio Salerno; Maria Oliviero; Ernesto Di Maio; Paolo A. Netti; Cristina Rofani; Alessia Colosimo; Valentina Guida; Bruno Dallapiccola; Paolo Palma; Emidio Procaccini; Anna C. Berardi; Francesco Velardi; Anna Teti; Salvatore Iannace

The design of bioactive scaffold materials able to guide cellular processes involved in new-tissue genesis is key determinant in bone tissue engineering. The aim of this study was the design and characterization of novel multi-phase biomaterials to be processed for the fabrication of 3D porous scaffolds able to provide a temporary biocompatible substrate for mesenchymal stem cells (MSCs) adhesion, proliferation and osteogenic differentiation. The biomaterials were prepared by blending poly(ε-caprolactone) (PCL) with thermoplastic zein (TZ), a thermoplastic material obtained by de novo thermoplasticization of zein. Furthermore, to bioactivate the scaffolds, microparticles of osteoconductive hydroxyapatite (HA) were dispersed within the organic phases. Results demonstrated that materials and formulations strongly affected the micro-structural properties and hydrophilicity of the scaffolds and, therefore, had a pivotal role in guiding cell/scaffold interaction. In particular, if compared to neat PCL, PCL–HA composite and PCL/TZ blend, the three-phase PCL/TZ–HA showed improved MSCs adhesion, proliferation and osteogenic differentiation capability, thus demonstrating potential for bone regeneration.


BMC Medical Genetics | 2007

Functional analysis of splicing mutations in exon 7 of NF1 gene.

Irene Bottillo; Alessandro De Luca; Annalisa Schirinzi; Valentina Guida; Isabella Torrente; Stefano Calvieri; Cristina Gervasini; Lidia Larizza; Antonio Pizzuti; Bruno Dallapiccola

BackgroundNeurofibromatosis type 1 is one of the most common autosomal dominant disorders, affecting about 1:3,500 individuals. NF1 exon 7 displays weakly defined exon-intron boundaries, and is particularly prone to missplicing.MethodsIn this study we investigated the expression of exon 7 transcripts using bioinformatic identification of splicing regulatory sequences, and functional minigene analysis of four sequence changes [c.910C>T (R304X), c.945G>A/c.946C>A (Q315Q/L316M), c.1005T>C (N335N)] identified in exon 7 of three different NF1 patients.ResultsOur results detected the presence of three exonic splicing enhancers (ESEs) and one putative exonic splicing silencer (ESS) element. The wild type minigene assay resulted in three alternative isoforms, including a transcript lacking NF1 exon 7 (NF1ΔE7). Both the wild type and the mutated constructs shared NF1ΔE7 in addition to the complete messenger, but displayed a different ratio between the two transcripts. In the presence of R304X and Q315Q/L316M mutations, the relative proportion between the different isoforms is shifted toward the expression of NF1ΔE7, while in the presence of N335N variant, the NF1ΔE7 expression is abolished.ConclusionIn conclusion, it appears mandatory to investigate the role of each nucleotide change within the NF1 coding sequence, since a significant proportion of NF1 exon 7 mutations affects pre-mRNA splicing, by disrupting exonic splicing motifs and modifying the delicate balance between aberrantly and correctly spliced transcripts.

Collaboration


Dive into the Valentina Guida's collaboration.

Top Co-Authors

Avatar

Bruno Dallapiccola

Sapienza University of Rome

View shared research outputs
Top Co-Authors

Avatar

Alessandro De Luca

Casa Sollievo della Sofferenza

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Bruno Marino

Sapienza University of Rome

View shared research outputs
Top Co-Authors

Avatar

Isabella Torrente

Sapienza University of Rome

View shared research outputs
Top Co-Authors

Avatar

Rosangela Ferese

Casa Sollievo della Sofferenza

View shared research outputs
Top Co-Authors

Avatar

Bruno Dallapiccola

Sapienza University of Rome

View shared research outputs
Top Co-Authors

Avatar

Federica Consoli

Casa Sollievo della Sofferenza

View shared research outputs
Top Co-Authors

Avatar

Liborio Stuppia

University of Chieti-Pescara

View shared research outputs
Researchain Logo
Decentralizing Knowledge