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Dive into the research topics where Valentino Romano is active.

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Featured researches published by Valentino Romano.


American Journal of Human Genetics | 2000

Tracing European Founder Lineages in the Near Eastern mtDNA Pool

Martin B. Richards; Vincent Macaulay; Eileen Hickey; Emilce Vega; Bryan Sykes; Valentina Guida; Chiara Rengo; Daniele Sellitto; Fulvio Cruciani; Toomas Kivisild; Richard Villems; Mark G. Thomas; Serge Rychkov; Oksana Rychkov; Yuri Rychkov; Mukaddes Gölge; Dimitar Dimitrov; Emmeline W. Hill; Daniel G. Bradley; Valentino Romano; Francesco Calì; Giuseppe Vona; Andrew G. Demaine; S.S. Papiha; Costas Triantaphyllidis; Gheorghe Stefanescu; Jiři Hatina; Michele Belledi; Anna Di Rienzo; Andrea Novelletto

Founder analysis is a method for analysis of nonrecombining DNA sequence data, with the aim of identification and dating of migrations into new territory. The method picks out founder sequence types in potential source populations and dates lineage clusters deriving from them in the settlement zone of interest. Here, using mtDNA, we apply the approach to the colonization of Europe, to estimate the proportion of modern lineages whose ancestors arrived during each major phase of settlement. To estimate the Palaeolithic and Neolithic contributions to European mtDNA diversity more accurately than was previously achievable, we have now extended the Near Eastern, European, and northern-Caucasus databases to 1,234, 2, 804, and 208 samples, respectively. Both back-migration into the source population and recurrent mutation in the source and derived populations represent major obstacles to this approach. We have developed phylogenetic criteria to take account of both these factors, and we suggest a way to account for multiple dispersals of common sequence types. We conclude that (i) there has been substantial back-migration into the Near East, (ii) the majority of extant mtDNA lineages entered Europe in several waves during the Upper Palaeolithic, (iii) there was a founder effect or bottleneck associated with the Last Glacial Maximum, 20,000 years ago, from which derives the largest fraction of surviving lineages, and (iv) the immigrant Neolithic component is likely to comprise less than one-quarter of the mtDNA pool of modern Europeans.


American Journal of Human Genetics | 1998

A European multicenter study of phenylalanine hydroxylase deficiency: Classification of 105 mutations and a general system for genotype-based prediction of metabolic phenotype

Per Guldberg; Francoise Rey; Johannes Zschocke; Valentino Romano; Baudouin François; Luc Michiels; Kurt Ullrich; Georg F. Hoffmann; Peter Burgard; H. Schmidt; Concetta Meli; Enrica Riva; Irma Dianzani; Alberto Ponzone; Jean Rey; Flemming Güttler

Phenylketonuria (PKU) and mild hyperphenylalaninemia (MHP) are allelic disorders caused by mutations in the gene encoding phenylalanine hydroxylase (PAH). Previous studies have suggested that the highly variable metabolic phenotypes of PAH deficiency correlate with PAH genotypes. We identified both causative mutations in 686 patients from seven European centers. On the basis of the phenotypic characteristics of 297 functionally hemizygous patients, 105 of the mutations were assigned to one of four arbitrary phenotype categories. We proposed and tested a simple model for correlation between genotype and phenotypic outcome. The observed phenotype matched the predicted phenotype in 79% of the cases, and in only 5 of 184 patients was the observed phenotype more than one category away from that expected. Among the seven contributing centers, the proportion of patients for whom the observed phenotype did not match the predicted phenotype was 4%-23% (P<.0001), suggesting that differences in methods used for mutation detection or phenotype classification may account for a considerable proportion of genotype-phenotype inconsistencies. Our data indicate that the PAH-mutation genotype is the main determinant of metabolic phenotype in most patients with PAH deficiency. In the present study, the classification of 105 PAH mutations may allow the prediction of the biochemical phenotype in >10,000 genotypes, which may be useful for the management of hyperphenylalaninemia in newborns.


Human Immunology | 2001

Human Y-chromosome variation in the western mediterranean area: Implications for the peopling of the region

Rosaria Scozzari; Fulvio Cruciani; Alessandra Pangrazio; Piero Santolamazza; Giuseppe Vona; Pedro Moral; Veronica Latini; Laurent Varesi; Marc Memmi; Valentino Romano; Giacomo De Leo; Massimo Gennarelli; Jadwiga Jaruzelska; Richard Villems; Jüri Parik; Vincent Macaulay; Antonio Torroni

Y-chromosome variation was analyzed in a sample of 1127 males from the Western Mediterranean area by surveying 16 biallelic and 4 multiallelic sites. Some populations from Northeastern Europe and the Middle East were also studied for comparison. All Y-chromosome haplotypes were included in a parsimonious genealogic tree consisting of 17 haplogroups, several of which displayed distinct geographic specificities. One of the haplogroups, HG9.2, has some features that are compatible with a spread into Europe from the Near East during the Neolithic period. However, the current distribution of this haplogroup would suggest that the Neolithic gene pool had a major impact in the eastern and central part of the Mediterranean basin, but very limited consequences in Iberia and Northwestern Europe. Two other haplogroups, HG25.2 and HG2.2, were found to have much more restricted geographic distributions. The first most likely originated in the Berbers within the last few thousand years, and allows the detection of gene flow to Iberia and Southern Europe. The latter haplogroup is common only in Sardinia, which confirms the genetic peculiarity and isolation of the Sardinians. Overall, this study demonstrates that the dissection of Y-chromosome variation into haplogroups with a more restricted geographic distribution can reveal important differences even between populations that live at short distances, and provides new clues to their past interactions.


Neuroreport | 2000

Dramatic brain aminergic deficit in a genetic mouse model of phenylketonuria.

Stefano Puglisi-Allegra; Simona Cabib; Tiziana Pascucci; Rossella Ventura; Francesco Calì; Valentino Romano

Clinical data suggest that brain catecholamines and serotonin are deficient in phenylketonuria (PKU), an inherited metabolic disorder that causes severe mental retardation and neurological disturbances. To test this hypothesis, brain tissue levels of dopamine (DA), norepinephrine (NE), 5-hydroxytryptamine (5-HT) and their metabolites were evaluated in the genetic mouse model of PKU (Pahenu2). Results indicated a significant reduction of 5-HT levels and metabolism in prefrontal cortex (pFC), cingulate cortex (Cg), nucleus accumbens (NAc), caudate putamen (CP), hippocampus (HIP) and amygdala (AMY). NE content and metabolism were reduced in pFC, Cg, AMY and HIP. Finally, significantly reduced DA content and metabolism was observed in pFC, NAc, CP and AMY. In pFC, NAc and CP there was also a marked reduction of DA release.


The Lancet | 1990

Molecular analysis of aldolase B genes in hereditary fructose intolerance

Nicholas C.P. Cross; Timothy M. Cox; R. de Franchis; Gianfranco Sebastio; C. Dazzo; Dean R. Tolan; C. Grégori; M. Odievre; M. Vidailhet; Valentino Romano; G. Mascali; Corrado Romano; Salvatore Musumeci; B. Steinmann; R. Gitzelmann

The molecular basis of hereditary fructose intolerance (HFI) was studied in 50 subjects (41 pedigrees, 82 apparently independent mutant alleles of aldolase B) by direct analysis of aldolase B genes amplified by means of the polymerase chain reaction. The mutation A149P (ala 149----pro) was found in 67% of alleles but was significantly more common in patients from northern than from southern Europe. Two other point mutations of aldolase B were identified. A174D (C----A; ala 174----asp) was found in subjects from Italy, Switzerland, and Yugoslavia (overall frequency 16%) but not in those from the United Kingdom, France, or the United States. L288 delta C carried a single base-pair deletion causing frameshift at codon 288 and was restricted to Sicilian subjects. By testing for these mutations in amplified DNA with a limited panel of allele-specific oligonucleotides, more than 95% of HFI patients will be susceptible to genetic diagnosis.


Annals of Human Genetics | 2006

Population Structure in the Mediterranean Basin: A Y Chromosome Perspective

Cristian Capelli; Nicola Redhead; Valentino Romano; Francesco Calì; Gérard Lefranc; Valérie Delague; André Mégarbané; Alex E. Felice; V. L. Pascali; P. I. Neophytou; Z. Poulli; Andrea Novelletto; Patrizia Malaspina; L. Terrenato; A. Berebbi; M. Fellous; Mark G. Thomas; David B. Goldstein

The Mediterranean region has been characterised by a number of pre‐historical and historical demographic events whose legacy on the current genetic landscape is still a matter of debate. In order to investigate the degree of population structure across the Mediterranean, we have investigated Y chromosome variation in a large dataset of Mediterranean populations, 11 of which are first described here. Our analyses identify four main clusters in the Mediterranean that can be labelled as North Africa, Arab, Central‐East and West Mediterranean. In particular, Near Eastern samples tend to separate according to the presence of Arab Y chromosome lineages, suggesting that the Arab expansion played a major role in shaping the current genetic structuring within the Fertile Crescent.


European Journal of Human Genetics | 2009

Differential Greek and northern African migrations to Sicily are supported by genetic evidence from the Y chromosome

Cornelia Di Gaetano; Nicoletta Cerutti; F. Crobu; Carlo Robino; S. Inturri; Sarah Gino; Simonetta Guarrera; Peter A. Underhill; Roy King; Valentino Romano; Francesco Calì; Mauro Gasparini; Giuseppe Matullo; Alfredo Salerno; Carlo Torre; Alberto Piazza

The presence or absence of genetic heterogeneity in Sicily has long been debated. Through the analysis of the variation of Y-chromosome lineages, using the combination of haplogroups and short tandem repeats from several areas of Sicily, we show that traces of genetic flows occurred in the island, due to ancient Greek colonization and to northern African contributions, are still visible on the basis of the distribution of some lineages. The genetic contribution of Greek chromosomes to the Sicilian gene pool is estimated to be about 37% whereas the contribution of North African populations is estimated to be around 6%.In particular, the presence of a modal haplotype coming from the southern Balkan Peninsula and of its one-step derivates associated to E3b1a2-V13, supports a common genetic heritage between Sicilians and Greeks. The estimate of Time to Most Recent Common Ancestor is about 2380 years before present, which broadly agrees with the archaeological traces of the Greek classic era. The Eastern and Western part of Sicily appear to be significantly different by the χ2-analysis, although the extent of such differentiation is not very high according to an analysis of molecular variance. The presence of a high number of different haplogroups in the island makes its gene diversity to reach about 0.9. The general heterogeneous composition of haplogroups in our Sicilian data is similar to the patterns observed in other major islands of the Mediterranean, reflecting the complex histories of settlements in Sicily.


Annals of Human Genetics | 2000

Patterns of male‐specific inter‐population divergence in Europe, West Asia and North Africa

Patrizia Malaspina; Fulvio Cruciani; Piero Santolamazza; Antonio Torroni; A. Pangrazio; Nejat Akar; V. Bakalli; Radim Brdicka; Jadwiga Jaruzelska; Andrey I. Kozlov; B. Malyarchuk; Syed Qasim Mehdi; Michalodimitrakis E; Laurent Varesi; Marc Memmi; Giuseppe Vona; Richard Villems; Jüri Parik; Valentino Romano; Mihaela Stefan; M. Stenico; L. Terrenato; Andrea Novelletto; Rosaria Scozzari

We typed 1801 males from 55 locations for the Y‐specific binary markers YAP, DYZ3, SRY10831 and the (CA)n microsatellites YCAII and DYS413. Phylogenetic relationships of chromosomes with the same binary haplotype were condensed in seven large one‐step networks, which accounted for 95% of all chromosomes. Their coalescence ages were estimated based on microsatellite diversity. The three largest and oldest networks undergo sharp frequency changes in three areas. The more recent network 3.1A clearly discriminates between Western and Eastern European populations. Pairwise Fst showed an overall increment with increasing geographic distance but with a slope greatly reduced when compared to previous reports. By sectioning the entire data set according to geographic and linguistic criteria, we found higher Fst‐on‐distance slopes within Europe than in West Asia or across the two continents.


Behavior Genetics | 2003

The behavioral profile of severe mental retardation in a genetic mouse model of phenylketonuria.

Simona Cabib; Tiziana Pascucci; Rossella Ventura; Valentino Romano; Stefano Puglisi-Allegra

Pahenu2 mice, created by chemically induced genetic mutation, are characterized by biochemical phenotypes closely resembling untreated human phenylketonuria (PKU). However, studies conducted in adult Pahenu2 mice have shown no indices of the severe mental retardation that characterizes untreated PKU. The present experiments explored recognition of novel spatial and nonspatial information in Pahenu2 mice by two nonassociative tests that do not use explicit reinforcement and avoid lengthy training. Moreover, we evaluated emotional reactivity by the Elevated Plus Maze. Finally, the performance of affected mutants was compared with that of their unaffected and heterozygous littermates and also with that of mice of the C57BL/6 (C57) inbred strain, an increasingly used background for genetic targeted organisms, and with DBA/2 (DBA) mice, known for their nonpathological deficits in spatial learning. The results demonstrated that mutant Pahenu2 mice are characterized by deficits involving both spatial and nonspatial recognition, that are not related to motor impairment or to high emotional reactivity to novelty. These results indicate that Pahenu2 mice show pathological cognitive deficits and support their use to test hypotheses about neurodevelopmental disturbances involved in mental retardation.


International Journal of Legal Medicine | 2002

Continental and subcontinental distributions of mtDNA control region types

Peter Forster; Francesco Calì; Arne Röhl; Ene Metspalu; Rosalba D’Anna; Mario G. Mirisola; Giacomo De Leo; Anna Flugy; Alfredo Salerno; Giovanni Ayala; Anastasia Kouvatsi; Richard Villems; Valentino Romano

When the mtDNA profile of a crime scene matches that of a suspect, it is necessary to determine the probability of a chance match by consulting the frequencies of the identified allele in a “reference population”. The ceiling principle suggests that that population should be chosen in which the allele of the suspect is found at the highest frequency, in order to give the suspect the maximum benefit of doubt. Recently, we advocated the use of a worldwide mitochondrial database combined with a geographical information system to identify the regions of the world with the highest frequencies of matching mtDNA types. Here, we demonstrate that the alternative approach of defining a ceiling reference population on the basis of continent or phenotype (race) is too coarse for a non-negligible percentage of mtDNA control region types.

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Paolo Bosco

Istituto Nazionale di Fisica Nucleare

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