Valeria A. Guinder

The role of Sarcocornia perennis and tidal flooding on sediment biogeochemistry in a South American wetland

Abstract The roles of Sarcocornia perennis and tidal flooding on sediment biogeochemistry were evaluated within a wetland in the Bahía Blanca estuary. pH and Eh were measured in sediments while particulate organic carbon (POC) and dissolved inorganic nutrient concentrations were determined in porewater, at three sites with different conditions according to vegetation and flooding. Grain size was also analysed. pH varied in a narrow range (7–8.2) and was lower in the vegetated site. Eh values (50–250 mV) imply that sediment conditions were moderately reduced, in agreement with the relatively high percentage of sand; it was influenced by both factors. POC concentration was high (26.24±1.62 mg/l), especially at the vegetated site. The concentrations of ammonium and nitrate were similar (21.30±1.83 and 18.77±3.06 µmol/l, respectively) and not affected by flooding; only nitrate was affected by vegetation. Phosphate was rather constant (13.43±1.19 µmol/l) and affected mainly by flooding. Silicate was high (566.45±76.06 µmol/l) and not affected by either factor. These results suggest that the sediment biogeochemistry of this environment is significantly influenced by flooding and, especially, by S. perennis, as vegetation affected a higher number of parameters.

Microplankton Dynamics Under Heavy Anthropogenic Pressure: the Case of the Bahía Blanca Estuary, Southwestern Atlantic Ocean

Quantifying biotic feedbacks in response to environmental signals is fundamental to assess ecosystem perturbation. We analyzed the joint effects of eutrophication, derived from sewage pollution, and climate at the base of the pelagic food web in the Bahía Blanca Estuary (SW Atlantic Ocean). A two-year survey of environmental conditions and microplankton communities was conducted in two sites affected by contrasting anthropogenic eutrophication conditions. Under severe eutrophication, we found higher phytoplankton abundance consistently dominated by smaller sized, non siliceous species, while microzooplankton abundance remained lower and nutrient stoichiometry showed conspicuous deviations from the Redfield ratio. Phytoplankton growth in such conditions appeared controlled by phosphorous. In turn, microplankton biomass and phytoplankton size ratio (<20μm:>20μm) displayed a saturation relationship with nutrients in the highly eutrophic area, although mean phytoplankton growth was similar in both eutrophic systems. The strength of links within the estuarine network, quantified through path analysis, showed enhanced relationships under larger anthropogenic eutrophication, which fostered the climate influence on microplankton communities. Our results show conspicuous effects of severe sewage pollution on the ecological stoichiometry, i.e., N and P excess with respect to Si, altering nutrient ratios for microplankton communities. This warns on wide consequences on food web dynamics and ultimately in ecosystem assets of coastal pelagic environments.

Short-term variability in the phytoplankton and physico-chemical variables in a high-tidal regime, Bahía Blanca Estuary, Argentina

Corresponding autor: vguinder@criba.edu.ar Hydrological processes such as advection, resuspension, estuarine circulation, internal waves, tidal stirring, vertical mixing and stratification are the main physical forces affecting the phytoplankton distribution in estuaries (DENMAN; GARGETT, 1983; CLOERN et al., 1989; CUSHING 1989; DE JONGE; VAN BEUSEKOM, 1992). Vertical and horizontal transport of phytoplankton in coastal areas could be induced by winds, water mass intrusions from the open sea, turbulence, freshwater inflow and tides (CLOERN et al., 1989; LI; SMAYDA, 2001; BRUNET; LIZON, 2003; HAGY III et al., 2005; POPOVICH; MARCOVECCHIO, 2008; POPOVICH et al., 2008b) and these sorts of transport are related to biomass accumulation and dispersion. Many studies have revealed intratidal variations in phytoplankton dynamics (e.g. DEMERS; LEGENDRE, 1979; DEMERS et al., 1986; DUSTAN; PINCKNEY, 1989; BRUNET; LIZON, 2003) and even differences in biomass between micro and macrotidal estuaries (MONBET, 1992). Moreover, phytoplankton patchiness in estuaries has been associated with the physical influence of tides (DUSTAN; PINCKNEY, 1989). In estuaries characterized by high-tidal energy, a steady phytoplankton composition over short time periods might be expected owing to the intense mixing processes continuously occurring in the water column. However, heterogeneous distributions of phytoplankton cells in the water column on different temporal and spatial scales have been observed (e.g. CUSHING, 1989; BRUNET; LIZON, 2003). Many studies have used the biomass (estimated by the chlorophyll a concentration) as the main intrinsic property to describe the phytoplankton community dynamics during tidal cycles (DENMAN, 1977; FRECHETTE; LEGENDRE, 1982, CLOERN et al., 1989; DUSTAN; PINCKNEY, 1989; MONBET 1992), without taking into consideration the species-specific patterns related to these regular short-time events. Hence, studies based only on phytoplankton biomass variability throughout tidal cycles may overlook the short-term temporal changes in the species composition. The Bahia Blanca Estuary constitutes a mesotidal system with a semidiurnal cycle, the tides being the single most important input of energy (PERILLO; PICCOLO, 1991; PERILLO et al., 2004). The temporal and spatial dynamics of the phytoplankton in the estuary have been extensively studied (GAYOSO, 1998, 1999; POPOVICH; MARCOVECCHIO, 2008; POPOVICH et al. 2008a, 2008b) but none of these works has considered the short-term variability associated with the tidal cycle. The main goal of this study was to describe the temporal phytoplankton and physico-chemical dynamics over a short-time period at a fixed station under strong tidal influence. This study presents the first integrated description of daily phytoplankton-community dynamics together with physical and chemical variables in the Bahia Blanca Estuary. Beside its local relevance, it is also a contribution to the understanding of the short-term variability in estuaries characterized by high tidal energy. Bahia Blanca is a coastal plain estuary (38°45´S; 62°22´W) on the Atlantic coast of Argentina (Fig. 1). Tidal currents are reversible with maximum surface velocities of about 1.3 m s

Time-varying environmental control of phytoplankton in a changing estuarine system

Estuaries are among the most valuable aquatic systems by their services to human welfare. However, increasing human activities at the watershed along with the pressure of climate change are fostering the co-occurrence of multiple environmental drivers, and warn of potential negative impacts on estuaries resources. At present, no clear understanding of how coastal ecosystems will respond to the non-stationary effect of multiple drivers. Here we analysed the temporal interaction among multiple environmental drivers and their changing priority on shaping phytoplankton response in the Bahía Blanca Estuary, SW Atlantic Ocean. The interaction among environmental drivers and the number of significant direct and indirect effects on chlorophyll concentration increased over time in concurrence with enhanced anthropogenic stress, changing winter climate and wind patterns. Over the period 1978-1993, proximal variables such as nutrients, water temperature and salinity, showed a dominant effect on chlorophyll, whereas in more recent years (1993-2009) climate signals (SAM and ENSO) boosted indirect effects through its influence on precipitation, wind, water temperature and turbidity. Turbidity emerged as the dominant driver of chlorophyll while in recent years acted synergistically with the concentration of dissolved nitrogen. As a result, chlorophyll concentration showed a significant negative trend and a loss of seasonal peaks reflecting a pronounced reorganisation of the phytoplankton community. We stress the need to account for the changing priority of drivers to understand, and eventually forecast, biological responses under projected scenarios of global anthropogenic change.

Heavy Metal Concentrations Found in Seston and Microplankton from an Impacted Temperate Shallow Estuary along the Southwestern Atlantic Ocean

ABSTRACT Fernández Severini, M.D.; Villagran, D.M.; Biancalana, F.; Berasategui, A.A.; Spetter, C.V.; Tartara, M.N.; Menéndez, M.C.; Guinder, V.A., and Marcovecchio, J.E., 2017. Heavy metal concentrations found in seston and microplankton from an impacted temperate shallow estuary along the southwestern Atlantic Ocean. Heavy metal concentrations (Cd, Cu, Cr, Fe, Mn, Ni, Pb, and Zn) were studied in the Bahía Blanca Estuary (BBE), one of the most anthropogenically disturbed estuaries in the SW Atlantic Ocean. This study evaluated metal concentrations in the microplankton and seston for the first time, as well as their role in the transport of contaminants in a disturbed coastal environment of the SW Atlantic. Spatial patterns of metals in suspended particulate matter (SPM; seston > 0.45 μm) and microplankton (20–200 μm) were analyzed at sampling sites located at sewage or industrial discharges and, also far from this area, during warm months in 2012 and 2013. Dissolved inorganic nutrients, particulate organic matter (POM), and chlorophyll a (Chl-a) were also analyzed. The POM and Chl-a concentrations were higher near to the sewage discharges; however, metals showed different concentrations between sampling site stations. Those located far from human disturbances showed similar levels to the sites at the sewage or industrial discharges in some cases. In general, the SPM had higher concentrations of metals than the microplankton. In particular, Cr presented important levels in this fraction, which was potentially associated with industrial discharges. On the other hand, a contrasting partition was found for Pb, Zn, and Cu that exhibited higher levels in the microplankton-net material. The high levels of Pb in the microplankton of the BBE may indicate a high availability of this metal in the environment and high uptake rates, with potential health risks to humans and marine life attributable to its toxic effects.

Phenological Changes of Blooming Diatoms Promoted by Compound Bottom-Up and Top-Down Controls

Understanding phytoplankton species-specific responses to multiple biotic and abiotic stressors is fundamental to assess phenological and structural shifts at the community level. Here, we present the case of Thalassiosira curviseriata, a winter-blooming diatom in the Bahía Blanca Estuary, Argentina, which displayed a noticeable decrease in the past decade along with conspicuous changes in phenology. We compiled interannual field data to assess compound effects of environmental variations and grazing by the invasive copepod Eurytemora americana. The two species displayed opposite trends over the period examined. The diatom decreased toward the last years, mainly during the winters, and remained relatively constant over the other seasons, while the copepod increased toward the last years, with an occurrence restricted to winter and early spring. A quantitative assessment by structural equation modeling unveiled that the observed long-term trend of T. curviseriata resulted from the synergistic effects of environmental changes driven by water temperature, salinity, and grazing. These results suggest that the shift in the abundance distribution of T. curviseriata toward higher annual ranges of temperature and salinity—as displayed by habitat association curves—constitutes a functional response to avoid seasonal overlapping with its predator in late winters. The observed changes in the timing and abundance of the blooming species resulted in conspicuous shifts in primary production pulses. Our results provide insights on mechanistic processes shaping the phenology and structure of phytoplankton blooms.

Reproductive trade-off of the copepod Acartia tonsa in a hypersaline estuary of the Southwestern Atlantic. Temporal variations in the morphology of eggs

ABSTRACT The purpose of the present work was to study the seasonal variations in egg production, morphology and hatching success in the cryptic species Acartia tonsa, taking into account variations in female size, population abundance and environmental factors in a turbid and hypersaline estuary. Sampling was performed during the austral warm (18–23°C and 32–36 salinity) and cold seasons (5–7°C; 32–38) in Bahía Blanca Estuary (BBE), Argentina, during 2007 and 2009. Field-collected females were incubated in the laboratory simulating in situ environmental conditions, and specimens from fixed samples were measured using optical and scanning electronic microscopy. Acartia tonsa’s marked seasonality in its reproductive traits was found to ensure its permanence in the water column all over the year. During the warm season, small-sized females were observed to invest their energy in the production of subitaneous eggs with high hatching success and smooth appearance (12.95 ± 2.38 eggs f−1 day−1 and specific egg production rate (SEP) of 16.57%C f−1 day−1). During the cold season, females invested C in body mass as well as in the production of resting eggs of three different morphotypes (6.56 ± 3.2 eggs f−1 day−1 and SEP of 7.37%C f−1 day−1). Although these morphotypes were found to show differences in surface ornamentation, they exhibited the same delayed hatching behaviour. The eggs with shorter spines were found to integrate the resting egg bank in BBE. Our findings confirming a delayed egg hatching behaviour and a great tolerance to low temperatures and high salinities in the A. tonsa population in BBE suggest that this possible strain is a valuable phenotype for aquaculture.

Amphidoma parvula (Amphidomataceae), a new planktonic dinophyte from the Argentine Sea

ABSTRACT Amphidoma is an old though poorly studied thecate dinophyte that has attracted attention recently as a potential producer of azaspiracids (AZA), a group of lipophilic phycotoxins. A new species, Amphidoma parvula, sp. nov. is described from the South Atlantic shelf of Argentina. With a Kofoidean thecal plate pattern Po, cp, X, 6′, 6′′, 6C, 5S, 6′′′, 2′′′′, the cultivated strain H-1E9 (from which the type material of Am. parvula, sp. nov. was prepared) shared the characteristic plate arrangement of Amphidoma each with six apical, precingular and postcingular plates. Amphidoma parvula, sp. nov. differs from other species of Amphidoma by a characteristic combination of small size (10.7–13.6 µm in length), ovoid shape, high length ratio between epitheca and hypotheca, and small length ratio between apical and precingular plates. Other morphological details, such as the number and arrangement of sulcal plates and the fine structure of the apical pore complex support the close relationship between Amphidoma and the other known genus of Amphidomataceae, Azadinium. However, Am. parvula, sp. nov. lacks a ventral pore, a characteristically structured pore found in all contemporary electron microscopy studies of Amphidoma and Azadinium. As inferred from liquid chromatography coupled with tandem mass spectrometry, Am. parvula, sp. nov. did not produce AZA in measurable amounts. Molecular phylogenetics confirmed the systematic placement of Am. parvula, sp. nov. in Amphidoma (as sister species of Amphidoma languida) and the Amphidomataceae. The results of this study have improved the knowledge of Amphidomataceae biodiversity.