Valérie Fritsch

Comparison of two amides as backbone replacement of the phosphodiester linkage in oligodeoxynucleotides

Abstract The two isomeric amide modifications 1 and 2 show similar effects on the melting temperature of RNA/DNA duplexes, when they replace the natural phosphodiester linkage in the DNA strand. The synthesis of the amide dimer 1 is presented.

Replacement of the phosphodiester linkage in oligonucleotides : comparison of two structural amide isomers

Abstract The two structural amide isomers 2 and 3 as backbone replacement in oligonucleotides lead to very similar results for the binding affinities (Tm) of the duplexes formed with their complementary RNA strands.

Amide backbone modifications for antisense oligonucleotides carrying potential intercalating substituents: Influence on the thermodynamic stability of the corresponding duplexes with RNA- and DNA- complements

Abstract The synthesis of the amide dimers 3–5 carrying a potential intercalating substituent on the nitrogen atom of the backbone is presented, together with the incorporation of these modifications into oligonucleotides, the resulting melting temperatures (Tms) of the corresponding duplexes with RNA- and DNA- complements, and a summary of molecular modeling studies.