Valery N. Khabashesku

Magnetocontrollability of Fe7C3@C superparamagnetic nanoparticles in living cells.

BackgroundA new type of superparamagnetic nanoparticles with chemical formula Fe7C3@C (MNPs) showed higher value of magnetization compared to traditionally used iron oxide-based nanoparticles as was shown in our previous studies. The in vitro biocompatibility tests demonstrated that the MNPs display high efficiency of cellular uptake and do not affect cyto-physiological parameters of cultured cells. These MNPs display effective magnetocontrollability in homogeneous liquids but their behavior in cytoplasm of living cells under the effect of magnetic field was not carefully analyzed yet.ResultsIn this work we investigated the magnetocontrollability of MNPs interacting with living cells in permanent magnetic field. It has been shown that cells were capable of capturing MNPs by upper part of the cell membrane, and from the surface of the cultivation substrate during motion process. Immunofluorescence studies using intracellular endosomal membrane marker showed that MNP agglomerates can be either located in endosomes or lying free in the cytoplasm. When attached cells were exposed to a magnetic field up to 0.15 T, the MNPs acquired magnetic moment and the displacement of incorporated MNP agglomerates in the direction of the magnet was observed. Weakly attached or non-attached cells, such as cells in mitosis or after cytoskeleton damaging treatments moved towards the magnet. During long time cultivation of cells with MNPs in a magnetic field gradual clearing of cells from MNPs was observed. It was the result of removing MNPs from the surface of the cell agglomerates discarded in the process of exocytosis.ConclusionsOur data allow us to conclude for the first time that the magnetic properties of the MNPs are sufficient for successful manipulation with MNP agglomerates both at the intracellular level, and within the whole cell. The structure of the outer shells of the MNPs allows firmly associate different types of biological molecules with them. This creates prospects for the use of such complexes for targeted delivery and selective removal of selected biological molecules from living cells.

Demand for New Instrumentation for Well Logging and Natural Formations Monitoring

Oil well logging is a powerful approach for high-precision evaluation and prediction of earth formation parameters. As a result, this approach has found wide-spread application in the characterization of oil fields. Here we describe several methods on a base of scintillation detectors allowing reliable characterization of the formation around wells. Demand for new scintillation materials to operate in an environment of deep wells is discussed.

Magnet-induced behavior of Iron Carbide (Fe7C3@C) Nanoparticles in the Cytoplasm of Living Cells

I. Alieva, I. Kireev, A. Rakhmanina, A. Garanina, O. Strelkova, O. Zhironkina, V. Cherepaninets, V. Davydov, V. Khabashesku, V. Agafonov, R. Uzbekov Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia L. F. Vereshchagin Institute for High Pressure Physics of the RAS, Troitsk, Russia Center for Technology Innovation, Baker Hughes Inc., Houston, TX, 77040, USA GREMAN, UMR CNRS 7347, Universite Francois Rabelais, 37200 Tours, France Faculte de Medecine, Universite Francois Rabelais, Tours, France Faculty of Bioengineering and Bioinformatics, Moscow State University, Moscow, Russia rustem.uzbekov@univ-tours.fr, viatcheslav.agafonov@univ-tours.fr

New superparamagnetic fluorescent Fe@C-C5ON2H10-Alexa Fluor 647 nanoparticles for biological applications

A. S. Garanina, I. I. Kireev, I. B. Alieva, A. G. Majouga, V. A. Davydov, S. Murugesan, V. N. Khabashesku, V. N. Agafonov, R. E. Uzbekov7,8∗ GREMAN, UMR CNRS 7347, Francois Rabelais University, Tours, France National University of Science and Technology “MISiS”, Moscow, Russia Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia Faculty of Chemistry, Lomonosov Moscow State University, Moscow, Russia L. F. Vereshchagin Institute for High Pressure Physics of the RAS, Moscow, Russia Center for Technology Innovation, Baker Hughes a GE Company, Houston, TX, USA Faculty of Medicine, Francois Rabelais University, Tours, France Faculty of Bioengineering and Bioinformatics, Moscow State University, Moscow, Russia ∗rustem.uzbekov@univ-tours.fr

Soft-Lithographic Patterning of Luminescent Carbon Nanodots Derived from Collagen Waste

Luminescent carbon dots (Cdots) synthesized using inexpensive precursors have inspired tremendous research interest because of their superior properties and applicability in various fields. In this work, we report a simple, economical, green route for the synthesis of multifunctional fluorescent Cdots prepared from a natural, low-cost source: collagen extracted from animal skin wastes. The as-synthesized metal-free Cdots were found to be in the size range of ∼1.2-9 nm, emitting bright blue photoluminescence with a calculated Cdot yield of ∼63%. Importantly, the soft-lithographic method used was inexpensive and yielded a variety of Cdot patterns with different geometrical structures and significant cellular biocompatibility. This novel approach to Cdot production highlights innovative ways of transforming industrial biowastes into advanced multifunctional materials which offer exciting potential for applications in nanophotonics and nanobiotechnology using a simple and scalable technique.