Vedran Lovric

Experimental Study and Analysis of Hydrostatic Pressure Sensitivity of Polymer Fibre Bragg Gratings

The intrinsic hydrostatic pressure sensitivity of polymer optical fiber Bragg grating (POFBG) with different diameters are investigated. POFBGs are inscribed in single-mode polymer fiber and etched down to different diameters. We have experimentally demonstrated that the material properties of the polymer optical fiber can change after etching and thus the etching procedure can have an impact on the pressure sensitivity of the POFBG. It is observed from the experimental results that hydrostatic pressure induces a positive wavelength shift to the POFBG and the pressure sensitivity of the POFBG shows significant increase as the fiber diameter reduces through etching. A pressure sensitivity of 0.20 pm/kPa is obtained for an unetched POFBG while for an etched POFBG with 55-μm diameter a sensitivity of 0.75 pm/kPa is observed. Temperature compensation techniques are also successfully implemented to extract the true intrinsic pressure sensitivity of the POFBG. Through this study, the intrinsic pressure sensitivity of POFBG with different diameters are obtained and also the significance of etching and its impact on pressure sensitivity is demonstrated. This information can lead to further research and development on high sensitivity pressure transducers based on etched POFBGs.

Effects of Demineralized Bone Matrix on Tendon-Bone Healing in an Intra-articular Rodent Model

Background: Techniques to improve and accelerate tendon-bone healing could be advantageous in anterior cruciate ligament (ACL) reconstruction. Effects of demineralized bone matrix (DBM) on intra-articular tendon-bone healing have not been examined. Hypothesis: Demineralized bone matrix has the potential to convey osteoinductive growth proteins to the site of healing at the tendon-bone interface. We hypothesized that the presence of DBM will result in more bone formation and hasten tendon-bone healing. Study Design: Controlled laboratory study. Methods: Fifty-six female athymic rnu/rnu (nude) rats were used. Rats were randomly allocated into 2 groups (control or treatment). The control group underwent an ACL reconstruction, while the treatment group had human DBM implanted in the tendon graft and bone tunnel before reconstruction. Rats were sacrificed at 2 (n = 8), 4 (n = 24), and 6 (n = 24) weeks for histological, and immunohistochemical (t = 2, 4, and 6 weeks), and biomechanical testing and micro–computed tomography (t = 4 and 6 weeks) end points. Results: Our findings suggest that in the presence of DBM, tendon-bone healing is augmented by increased woven bone formation and enhanced bone remodeling as indicated by histology and micro–computed tomography. This ultimately resulted in a statistically significant increase in peak load to failure of the tendon-bone interface at 4 weeks (DBM group: 5.96 ± 1.36 N; control group: 2.86 ± 0.7 N) and 6 weeks (DBM group: 9.13 ± 0.97 N; control group: 5.81 ± 1.1 N). Conclusion: Demineralized bone matrix at the tendon-bone interface promotes healing between the tendon and bone in a rodent ACL model. Clinical Relevance: Introduction of osteoinductive DBM at the tendon-bone interface during ACL reconstructive surgery may improve short-term outcomes.

How do porosity-inducing techniques affect antibiotic elution from bone cement? An in vitro comparison between hydrogen peroxide and a mechanical mixer

BackgroundIncreasing the porosity of an antibiotic-loaded cement spacer increases the antibiotic elution, but the correlation between porosity and antibiotic elution is not well documented. The purposes of this study was to attempt new porosity-increasing methods and to investigate the correlation between antibiotic elution and both total and surface porosity.Materials and methodsFive types of antibiotic-loaded bone cement (ALBC) using 2 g cefazolin and 40 g cement were prepared. Other than manual mixing, hydrogen peroxide was used as a foaming agent and a mixing drill piece was used as a mechanical device to try to induce porosity when mixing the cement. Elution of antibiotic into phosphate-buffered saline was measured from 1 h to 1 week. Surface porosity was calculated from density values which were measured with a density kit and an electronic balance, while total porosity was quantified using micro-computed tomography.ResultsWhen a mixing drill piece was used to induce porosity, we observed a significant increasin antibiotic elution compared to a manually mixed ALBC. On the other hand, hydrogen peroxide reduced the elution significantly. Mild correlation between the total amount of cluted in 1 week antibiotic elution and total porosity was observed.ConclusionsIn terms of improving elution, the mixing drill piece seemed to be efficient. A relationship between surface porosity and elution efficacy was not observed.

Intrinsic High-Sensitivity Sensors Based on Etched Single-Mode Polymer Optical Fibers

The significance of etched single-mode polymer optical fibers and their potential for the development of high-sensitivity sensors are presented. A polymethyl methacrylate-based single-mode polymer optical fiber is etched to various diameters and it is observed that etching can lead to change in the material properties of the fiber, such as Youngs modulus and thermal expansion coefficient. This can play a vital role in improving the intrinsic sensing capabilities of sensors based on etched polymer optical fiber. To demonstrate that the modified material properties of the etched polymer fiber can enhance its intrinsic sensing capabilities, sensing characteristics of etched polymer fiber Bragg gratings for strain, temperature, and pressure are obtained. From the results, it is confirmed that the sensors based on etched polymer fibers exhibit high intrinsic sensitivity compared with un-etched ones. The potential of developing a sensing system for simultaneous measurement of strain and temperature is also demonstrated.

High Intrinsic Sensitivity Etched Polymer Fiber Bragg Grating Pair for Simultaneous Strain and Temperature Measurements

A sensing configuration for simultaneous measurement of strain and temperature with enhanced intrinsic sensitivity based on a fiber Bragg grating (FBG) pair with one grating inscribed in the etched and the other in unetched polymer fiber region is demonstrated. A poly (methyl methacrylate) based single-mode polymer fiber is etched to different diameters, and it is observed that etching can lead to change in the material properties of the fiber, such as Youngs modulus and thermal expansion coefficient, which can play a vital role in improving its intrinsic sensing capabilities. Thus, exploiting the different strain and temperature sensitivities exhibited by etched and unetched polymer FBGs, strain and temperature can be simultaneously measured with very high sensitivity. Experimental results show that rms deviations of ±8.42 με and ±0.39 °C for strain and temperature, respectively, in a real simultaneous measurement. The effect of individual thermal and strain sensitivity coefficients on measurement accuracy is also analyzed.

Development of a Novel Model for the Assessment of Dead-Space Management in Soft Tissue

Following extensive surgical debridement in the treatment of infection, a “dead space” can result following surgical closure that can fill with hematoma, an environment conducive to bacterial growth. The eradication of dead space is essential in order to prevent recurrent infection. This study describes a novel small animal model to investigate dead-space management in muscle tissue. Two absorbable test materials were implanted in each animal; beads of calcium sulfate alone, and beads loaded with vancomycin and tobramycin. In-life blood samples and radiographs were taken from each animal following implantation. Animals were sacrificed at 1, 7, 21, 42, and 63 days post-operatively (n = 4), and implant sites were analysed by micro-computed tomography, histology and immunohistochemistry. Complete resorption was confirmed radiographically at 3 weeks post-implantation. Histologically, the host tissue response to both materials was identical, and subsequent healing at the implant sites was observed with no dead space remaining. Vancomycin was not detected in blood serum. However, peak tobramycin levels were detected in all animals at 6 hours post-implantation with no detectable levels in any animals at 72 hours post implantation. Serological inflammatory cytokine expression for IL-6, TNF-α and IL-1β indicated no unusual inflammatory response to the implanted materials or surgical procedure. The model was found to be convenient and effective for the assessment of implant materials for management of dead space in muscle tissue. The two materials tested were effective in resolving the surgically created dead space, and did not elicit any unexpected adverse host response.

Critical Size Bone Defect Healing Using Collagen-Calcium Phosphate Bone Graft Materials.

The need for bone graft materials to fill bony voids or gaps that are not related to the intrinsic stability of the bone that arise due to trauma, tumors or osteolysis remains a clinically relevant and significant issue. The in vivo response of collagen–tricalcium phosphate bone graft substitutes was evaluated in a critical size cancellous defect model in skeletally mature rabbits. While the materials were chemically virtually identical, new bone formation, implant resorption and local in vivo responses were significantly different. Differences in the in vivo response may be due, in part, collagen source and processing which influences resorption profiles. Continued improvements in processing and manufacturing techniques of collagen—tricalcium phosphate bone graft substitutes can result in osteoconductive materials that support healing of critical size bone defects even in challenging pre-clinical models.

Etching Process Related Changes and Effects on Solid-Core Single-Mode Polymer Optical Fiber Grating

Etching process related material and mechanical changes on solid-core singlemode polymer optical fiber (POF) and their influence on the characteristic properties of polymer fiber Bragg gratings (PFBGs) are studied. A poly(methyl methacrylate)-based POF is etched to different diameters, and it is experimentally demonstrated that etching can lead to a change in the Youngs modulus of the fiber. It is found that etching process induced material changes of the polymer fiber can enhance the reflectivity and inherent sensitivity of PFBGs. It is demonstrated that gratings based on etched POF exhibit higher reflectivity with a shorter exposure time compared with unetched ones. Highest reflectivity of 98.54% is observed within 7 seconds of exposure for a diameter of 85 μm. The stability of fabricated Bragg gratings is also studied. By tailoring the etching diameter of POF, Bragg gratings with high sensitivity and high reflectivity can be fabricated.

Effects of supercritical fluid CO2 and 25 kGy gamma irradiation on the initial mechanical properties and histological appearance of tendon allograft

Tendon allografts, when autograft options are limited or when obtaining an autograft is not aligned with the patients’ best interest, play an important role in tendon and ligament reconstruction. To minimize the risk of infectious disease transmission tissue banks perform screening tests and the allografts cleaned are sterilized. The current study examines and compares the initial mechanical properties and histological appearance of supercritical CO2 (SCCO2)-treated and gamma-irradiated porcine extensor tendons. Thirty intact porcine forelimb extensor tendons randomized equally into three groups: control group, gamma-irradiation group, and SCCO2-treated group. Once treated, histological assessment and histomorphologic measurements were made on the histological sections obtained from each tendon while stiffness and ultimate failure loads were evaluated from tensile testing. Histological evaluation of gamma-irradiated tendons showed significant disruption to the hierarchical morphology of the fascicle bundles, which was not evident in SCCO2-treated specimens. Histomorphologic measurements showed a significant increase for measured dead space (void) between tendon fibrils of the gamma-irradiated group comparing to both control and SCCO2 treated groups (p < 0.01). There was a significant reduction in the ultimate failure load for tendons treated by gamma-irradiation compared to the control group (p < 0.05). No statistically significant difference was detected between control and SCCO2-treated tendons in the ultimate failure load. Stiffness values were not significantly different between three-study groups. This study suggests that while gamma-irradiation has a deleterious effect on mechanical properties of tendon tissue, SCCO2 does not alter the biomechanical properties and the histological structure of porcine extensor tendons.

Application of Calcium Sulfate for Dead Space Management in Soft Tissue: Characterisation of a Novel In Vivo Response

Management of dead space (DS) is a fundamental aspect of surgery. Residual DS following surgery can fill with hematoma and provide an environment for bacterial growth, increasing the incidence of postoperative infection. Materials for managing DS include polymethyl-methacrylate (PMMA), which is nonresorbing and requires removal in a second surgical procedure. The use of calcium sulfate (CS) offers the advantage of being fully absorbed and does not require subsequent surgical removal. As CS has historically been used as a bone void filler, there are some concerns for the risk of heterotopic ossification (HO) when implanted adjacent to soft tissue. This study assessed the osteoinductive potential of CS and identified and characterised residual material present in muscle tissue using histology, energy-dispersive X-ray spectroscopy analysis, and scanning electron microscopy (SEM). CS beads with and without antibiotic were implanted in intramuscular sites in both athymic rats and New Zealand white rabbits. At 28 days after implantation in the rat model, no signs of osteoinduction were observed. In the rabbit model, at 21 days after implantation, almost complete bead absorption and presence of a “halo” of material in the surrounding muscle tissue were confirmed. Our results suggested that the halo of material was a calcium phosphate precipitate, not HO.