Veit Braun
Carl Zeiss AG
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Publication
Featured researches published by Veit Braun.
Journal of Molecular Biology | 2010
David Albesa-Jové; Thomas Bertrand; Elisabeth P. Carpenter; Gemma V. Swain; Jenson Lim; Jiancheng Zhang; Lesley F. Haire; Nishi Vasisht; Veit Braun; Anton Lange; Christoph Von Eichel-Streiber; Dmitri I. Svergun; Neil F. Fairweather; Katherine A. Brown
Clostridium difficile is a nosocomial bacterial pathogen causing antibiotic-associated diarrhea and fatal pseudomembranous colitis. Key virulence factors are toxin A and toxin B (TcdB), two highly related toxins that are members of the large clostridial toxin family. These large multifunctional proteins disrupt cell function using a glucosyltransferase domain that is translocated into the cytosol after vesicular internalization of intact holotoxin. Although substantial information about the biochemical mechanisms of intoxication exists, research has been hampered by limited structural information, particularly of intact holotoxin. Here, we used small-angle X-ray scattering (SAXS) methods to obtain an ab initio low-resolution structure of native TcdB, which demonstrated that this molecule is monomeric in solution and possesses a highly asymmetric shape with a maximum dimension of approximately 275 A. Combining this SAXS information with crystallographic or modeled structures of individual functional domains of TcdB reveals for the first time that the three-dimensional structure of TcdB is organized into four distinct structural domains. Structures of the N-terminal glucosyltransferase, the cysteine protease, and the C-terminal repeat region can be aligned within three domains of the SAXS envelope. A fourth domain, predicted to be involved in the translocation of the glucosyltransferase, appears as a large solvent-exposed protrusion. Knowledge of the shapes and relative orientations of toxin domains provides new insight into defining functional domain boundaries and provides a framework for understanding how potential intra-domain interactions enable conformational changes to propagate between domains to facilitate intoxication processes.
Journal of Bacteriology | 2001
Adam P. Roberts; Veit Braun; Christoph von Eichel-Streiber; Peter Mullany
Previous work has identified the conjugative transposon Tn5397 from Clostridium difficile. This element was shown to contain a group II intron. Tn5397 can be conjugatively transferred from C. difficile to Bacillus subtilis. In this work we show that the intron is spliced in both these hosts and that nonspliced RNA is also present. We constructed a mutation in the open reading frame within the intron, and this prevented splicing but did not prevent the formation of the circular form of the conjugative transposon (the likely transposition intermediate) or decrease the frequency of intergeneric transfer of Tn5397. Therefore, the intron is spliced, but splicing is not required for conjugation of Tn5397.
Journal of Bacteriology | 2004
Oliver Hasselmayer; Veit Braun; Claudia Nitsche; Michael Moos; Maja Rupnik; Christoph von Eichel-Streiber
Screening a Clostridium difficile strain collection for the chimeric element CdISt1, we identified two additional variants, designated CdISt1-0 and CdISt1-III. In in vitro assays, we could prove the self-splicing ribozyme activity of these variants. Structural comparison of all known CdISt1 variants led us to define four types of IStrons that we designated CdISt1-0 through CdISt1-III. Since CdISt1-0 encodes two complete transposase-like proteins (TlpA and TlpB), we suggest that it represents the original genetic element, hypothesized before to have originated by fusion of a group I intron and an insertion sequence element.
Archive | 1999
Veit Braun; Christoph von Eichel-Streiber
Archive | 2009
Eichel-Streiber Christoph Von; Michael Moos; Karina Gisch; Veit Braun
Archive | 2009
Veit Braun; Karina Gisch; Michael Moos; Christoph von Eichel-Streiber
Laboratoriumsmedizin-journal of Laboratory Medicine | 2008
Christoph von Eichel-Streiber; Veit Braun
Archive | 2008
Veit Braun; Christoph von Eichel-Streiber; Karina Gisch; Michael Moos
Archive | 2008
Veit Braun; Karina Gisch; Michael Moos; Christoph von Eichel-Streiber
Archive | 2007
Franz Paul Armbruster; Veit Braun; Christoph Von Eichel-Streiber; Ralf Jochem