Verena B Heuer

Exploring deep microbial life in coal-bearing sediment down to ~2.5 km below the ocean floor

A deep sleep in coal beds Deep below the ocean floor, microorganisms from forest soils continue to thrive. Inagaki et al. analyzed the microbial communities in several drill cores off the coast of Japan, some sampling more than 2 km below the seafloor (see the Perspective by Huber). Although cell counts decreased with depth, deep coal beds harbored active communities of methanogenic bacteria. These communities were more similar to those found in forest soils than in other deep marine sediments. Science, this issue p. 420; see also p. 376 Coal beds more than 2 kilometers below the seafloor host methanogenic bacteria related to those found in forest soils. [Also see Perspective by Huber] Microbial life inhabits deeply buried marine sediments, but the extent of this vast ecosystem remains poorly constrained. Here we provide evidence for the existence of microbial communities in ~40° to 60°C sediment associated with lignite coal beds at ~1.5 to 2.5 km below the seafloor in the Pacific Ocean off Japan. Microbial methanogenesis was indicated by the isotopic compositions of methane and carbon dioxide, biomarkers, cultivation data, and gas compositions. Concentrations of indigenous microbial cells below 1.5 km ranged from <10 to ~104 cells cm−3. Peak concentrations occurred in lignite layers, where communities differed markedly from shallower subseafloor communities and instead resembled organotrophic communities in forest soils. This suggests that terrigenous sediments retain indigenous community members tens of millions of years after burial in the seabed.

Acetogenesis in Deep Subseafloor Sediments of The Juan de Fuca Ridge Flank: A Synthesis of Geochemical, Thermodynamic, and Gene-based Evidence

In deep subsurface sediments of the Juan de Fuca Ridge Flank, porewater acetate that is depleted in 13 C relative to sedimentary organic matter indicates an acetogenic component to total acetate production. Thermodynamic calculations indicate common fermentation products or lignin monomers as potential substrates for acetogenesis. The classic autotrophic reaction may contribute as well, provided that dihydrogen (H 2 ) concentrations are not drawn down to the thermodynamic thresholds of the energetically more favorable processes of sulfate reduction and methanogenesis. A high diversity of novel formyl tetrahydrofolate synthetase (fhs) genes throughout the upper half of the sediment column indicates the genetic potential for acetogenesis. Our results suggest that a substantial fraction of the acetate produced in marine sediment porewaters may derive from acetogenesis, in addition to the conventionally invoked sources fermentation and sulfate reduction.

Processes and Signals of Nonsteady-State Diagenesis in Deep-Sea Sediments and their Pore Waters

Nonsteady-state conditions — induced by changes in the fluxes of electron donors and acceptors and environmental conditions — are shown to have been and to be still widespread in sediments of the equatorial and South Atlantic Ocean. Typical diagenetic phenomena initiated under such nonsteady-state conditions comprise the fixation and downward progression of redox boundaries and reaction fronts. Intervals most severely altered by diagenetic overprint often occur cyclically within the sedimentary record and are mostly associated with full glacial/interglacial transitions. The extent of post-depositional oxidation of organic carbon as well as the dissolution and reprecipitation of minerals across these glacial terminations was shown to depend on the overall sedimentation rate and the magnitude of change encountered in the various depositional and geochemical factors. A sedimentation rate of about 2 cm/kyr was confirmed to be the critical value below which no significant amounts of non-refractory organic carbon are preserved. The influence of climatically induced variations in environmental conditions is not restricted to the geochemical boundaries in the vicinity of the sediment surface (e.g. oxic/post-oxic and Fe redox boundary) but well extends into much deeper sediment sections — namely into the zone of anaerobic oxidation of methane (AOM). In this way, processes within the zone of AOM can produce a further profound diagenetic alteration of the sediment composition up to hundreds of thousands of years after initial deposition and thus a significantly delayed chemical log-in. The long-term utility of all primary and secondary signals — also those formed and initially preserved across the oxic/post-oxic and Fe redox boundaries — is ultimately controlled by the geochemical processes within and below the sulfate/methane transition (SMT). While dissolution of authigenic and productivity-related barite takes place in sulfate-depleted sediment sections, iron sulfides as well as sulfurized organic matter and associated trace elements have a high potential to survive burial below the SMT. Nonsteady-state diagenesis can be triggered not only by changes in conditions at the sediment/water interface like TOC input, sedimentation rate or O2 content of bottom water but also by processes in the underlying sediment — namely the formation and/or liberation of methane. Apart from the distinct alteration of the solid-phase composition, variations in the upward flux of methane also have a considerable impact on the shape of sulfate pore water profiles. Modelling the effects of such variations in methane flux on sulfate profiles has illustrated that considering possible nonsteady-state situations in the sediment/pore water system is of utmost importance for the interpretation of pore water data.

Carbon flow from volcanic CO2 into soil microbial communities of a wetland mofette

Effects of extremely high carbon dioxide (CO2) concentrations on soil microbial communities and associated processes are largely unknown. We studied a wetland area affected by spots of subcrustal CO2 degassing (mofettes) with focus on anaerobic autotrophic methanogenesis and acetogenesis because the pore gas phase was largely hypoxic. Compared with a reference soil, the mofette was more acidic (ΔpH ∼0.8), strongly enriched in organic carbon (up to 10 times), and exhibited lower prokaryotic diversity. It was dominated by methanogens and subdivision 1 Acidobacteria, which likely thrived under stable hypoxia and acidic pH. Anoxic incubations revealed enhanced formation of acetate and methane (CH4) from hydrogen (H2) and CO2 consistent with elevated CH4 and acetate levels in the mofette soil. 13CO2 mofette soil incubations showed high label incorporations with ∼512 ng 13C g (dry weight (dw)) soil−1 d−1 into the bulk soil and up to 10.7 ng 13C g (dw) soil−1 d−1 into almost all analyzed bacterial lipids. Incorporation of CO2-derived carbon into archaeal lipids was much lower and restricted to the first 10 cm of the soil. DNA-SIP analysis revealed that acidophilic methanogens affiliated with Methanoregulaceae and hitherto unknown acetogens appeared to be involved in the chemolithoautotrophic utilization of 13CO2. Subdivision 1 Acidobacteriaceae assimilated 13CO2 likely via anaplerotic reactions because Acidobacteriaceae are not known to harbor enzymatic pathways for autotrophic CO2 assimilation. We conclude that CO2-induced geochemical changes promoted anaerobic and acidophilic organisms and altered carbon turnover in affected soils.

Origin and fate of acetate in an acidic fen.

Acetate is a central intermediate in the anaerobic degradation of organic matter, and the resolution of its metabolism necessitates integrated strategies. This study aims to (1) estimate the contribution of acetogenesis to acetate formation in an acidic fen (pH ~ 4.9), (2) assess the genetic potential for acetogenesis targeting the fhs gene encoding formyltetrahydrofolate synthetase (FTHFS) and (3) unravel the in situ turnover of acetate using stable carbon isotope pore-water analysis. H(2)/CO(2)-supplemented peat microcosms yielded (13)C-depleted acetate (-37.2‰ vs. VPDB (Vienna Peedee belemnite standard) compared with -14.2‰ vs. VPDB in an unamended control), indicating the potential for H(2)-dependent acetogenesis. Molecular analysis revealed a high diversity and depth-dependent distribution of fhs phylotypes with the highest number of operational taxonomic units in 0-20 cm depth, but only few and distant relationships to known acetogens. In pore waters, acetate concentrations (0-170 μM) and δ(13)C-values varied widely (-17.4‰ to -3.4‰ vs. VPDB) and did not indicate acetogenesis, but pointed to a predominance of sinks, which preferentially consumed (12)C-acetate, like acetoclastic methanogenesis. However, depth profiles of methane and δ(13)C(CH4) revealed a temporarily and spatially restricted role of this acetate sink and suggest other processes like sulfate and iron reduction played an important role in acetate turnover.

Gas hydrate transect across Northern Cascadia Margin

Gas hydrate is a solid compound mainly comprised of methane and water that is stable under low temperature and high pressure conditions. Usually found in offshore environments with water depths exceeding about 500 meters and in arctic regions associated with permafrost, gas hydrates form an efficient storage system for natural gas. Hence, they may represent an important future energy resource [e.g., Kvenvolden, 1988]. Gas hydrates also form a natural geo-hazard, and may play a significant role in global climate change [e.g., Dillon et al., 2001].

Real-time drilling mud gas monitoring for qualitative evaluation of hydrocarbon gas composition during deep sea drilling in the Nankai Trough Kumano Basin

BackgroundIntegrated Ocean Drilling Program Expedition 338 was the second scientific expedition with D/V Chikyu during which riser drilling was conducted as part of the Nankai Trough Seismogenic Zone Experiment. Riser drilling enabled sampling and real-time monitoring of drilling mud gas with an onboard scientific drilling mud gas monitoring system (“SciGas”). A second, independent system was provided by Geoservices, a commercial mud logging service. Both systems allowed the determination of (non-) hydrocarbon gas, while the SciGas system also monitored the methane carbon isotope ratio (δ13CCH4). The hydrocarbon gas composition was predominated by methane (> 1%), while ethane and propane were up to two orders of magnitude lower. δ13CCH4 values suggested an onset of thermogenic gas not earlier than 1600 meter below seafloor. This study aims on evaluating the onboard data and subsequent geological interpretations by conducting shorebased analyses of drilling mud gas samples.ResultsDuring shipboard monitoring of drilling mud gas the SciGas and Geoservices systems recorded up to 8.64% and 16.4% methane, respectively. Ethane and propane concentrations reached up to 0.03 and 0.013%, respectively, in the SciGas system, but 0.09% and 0.23% in the Geoservices data. Shorebased analyses of discrete samples by gas chromatography showed a gas composition with ~0.01 to 1.04% methane, 2 – 18 ppmv ethane, and 2 – 4 ppmv propane. Quadruple mass spectrometry yielded similar results for methane (0.04 to 4.98%). With δD values between -171‰ and -164‰, the stable hydrogen isotopic composition of methane showed little downhole variability.ConclusionsAlthough the two independent mud gas monitoring systems and shorebased analysis of discrete gas sample yielded different absolute concentrations they all agree well with respect to downhole variations of hydrocarbon gases. The data point to predominantly biogenic methane sources but suggest some contribution from thermogenic sources at depth, probably due to mixing. In situ thermogenic gas production at depths shallower 2000 mbsf is unlikely based on in situ temperature estimations between 81°C and 85°C and a cumulative time-temperature index of 0.23. In conclusion, the onboard SciGas data acquisition helps to provide a preliminary, qualitative evaluation of the gas composition, the in situ temperature and the possibility of gas migration.