Veronika Morvai

Site-specific risk assessment in contaminated vegetable gardens

A field survey was carried on in Gyöngyösoroszi, Hungary, near to an abandoned lead/zinc mine to analyse the metal contamination of flooded and non-flooded vegetable gardens, and to evaluate the health risks to local population. Contamination levels of arsenic, cadmium, lead, mercury and zinc were measured in soil and homegrown vegetable samples and bioconcentration factors and hazard indices were calculated. The high metal contents of flooded vegetable gardens were caused by floods, the results indicated significant differences between flooded and non-flooded vegetable gardens. The most accumulating vegetable was sorrel, the most mobile elements were cadmium and lead. Arsenic was not available for vegetables. The health risk was calculated for two exposure routes: ingestion of soil and ingestion of vegetables. The site-specific exposure parameters were established after a population based survey and a special equation was created to calculate the health risk due to homegrown vegetable consumption. The highest risk was associated with ingestion of vegetables, the most hazardous element being lead. The hazard index did not exceed the threshold value of one in flooded or non-flooded gardens. The analyses of health risk indicated that despite the high metal concentrations of soil the contamination of vegetable gardens does not pose an unacceptable risk to the inhabitants of the village.

Effects of cobalt sulfate on prenatal development of mice, rats, and rabbits, and on early postnatal development of rats

The effects of cobalt sulfate administered to pregnant C57Bl mice, OFA-SD rats, and New Zealand rabbits was studied on fetal and postnatal offspring. Cobalt concentration in the maternal blood was increased in proportion to the administered doses. Cobalt crossed the placenta and appeared in the fetal blood and amniotic fluid. Regardless of the administered dose of cobalt sulfate, cobalt concentration in the blood peaked 2 h after administration. Cobalt produced dose-dependent maternal toxicity and was found to be embryotoxic in all three species, as evidenced by elevated frequency of fetuses with body weight or skeletal retardation and embryolethality. Cobalt increased the frequency of major anomalies significantly in mice and rats, with anomalies of the eyes, kidneys, skull, spine, and sternum in mice, and anomalies of the urogenital system in rats. Cobalt sulfate was not teratogenic in rabbits. Intra-amnial administration of cobalt sulfate produced a dose-dependent increase of the frequency of dead fetuses, and weight retardation of the live fetuses. The direct cytotoxic effect probably plays a role in the embryotoxic and teratogenic effects of cobalt. The postnatal examinations revealed a decrease of the perinatal index in the treated group. The body weight of the pups in the treated group was lower during wk 1 of life, but no difference was found between the control and treated by the end of wk 2. Eye opening was completed in the usual time period in both groups, while time of appearance of the teeth, descending of the testes, shaping of ears, and development of hearing was delayed in the treated group. The development of muscle strength and of the locomotor system was delayed. All the functions studied (forward movement, swimming, righting reflex) normalized by postnatal d 21, with the exception of muscle strength. It was concluded that cobalt sulfate exposure decreases the perinatal viability of the fetuses, but the functions of the surviving fetuses with perinatal retardation become compensated by postnatal wk 2-3. The development of fetuses is undisturbed thereafter.

Embryotoxic and teratogenic effects of indium chloride in rats and rabbits.

Daily indium chloride doses of control (0), 50, 100, 200, or 400 mg/kg were administered orally to Sprague-Dawley rats by gavage, on d 6-15 of gestation, and daily metal doses of control (0), 50, 100, or 200 mg/kg were administered to New Zealand rabbits on d 6-20 of gestation. Further groups of pregnant rats were treated with control (0) or 400 mg/kg indium chloride orally on one of d 8, 9, 10, 11, 12, 13, 14, or 15 of gestation. The dams and fetuses were examined on d 21 (rats) and 30 (rabbits) of gestation, using standard teratological methods. Indium concentration was determined in the maternal and fetal blood, as well as in the amniotic fluid, by atomic absorption spectrometry. Indium was found to cross the placenta and appeared in fetal blood in proportion to the metal concentration of the maternal blood. In the amniotic fluid, indium concentrations remained below the detection limit. In rats, indium chloride produced dose-dependent maternal toxic effects, with a dose of 400 mg/kg inducing embryotoxicity (embryolethality) and teratogenicity. Doses of 200 and 100 mg/kg were embryotoxic (retarding) and teratogenic, causing skeletal and visceral anomalies in addition to external anomalies (rudimentary or missing tail, syndactylia, clubfoot, exencephalia) in rats. In rabbits, 200 mg/kg indium chloride was lethal for the dams and the embryos (some of the animals died, and the number of abortions and full resorptions increased). This dose was found to be teratogenic (caused gross renal anomalies) and increased the frequency of fetuses with skeletal retardation. In rats, the effects of indium chloride causing fetal retardation was found to be independent of exposure time. The teratogenic effects were the highest on d 11 and 12 of gestation, when indium chloride caused gross external malformations. Data suggest that the teratogenic effects of indium chloride can be attributed primarily to a direct cytotoxic action of indium resulting from placental transfer, but the effect is not a selective one, as it appears only in the presence of maternal toxic effects.Daily indium chloride doses of control (0), 50, 100, 200, or 400 mg/kg were administered orally to Sprague-Dawley rats by gavage, on d 6-15 of gestation, and daily metal doses of control (0), 50, 100, or 200 mg/kg were administered to New Zealand rabbits on d 6-20 of gestation. Further groups of pregnant rats were treated with control (0) or 400 mg/kg indium chloride orally on one of d 8, 9, 10, 11, 12, 13, 14, or 15 of gestation. The dams and fetuses were examined on d 21 (rats) and 30 (rabbits) of gestation, using standard teratological methods. Indium concentration was determined in the maternal and fetal blood, as well as in the amniotic fluid, by atomic absorption spectrometry. Indium was found to cross the placenta and appeared in fetal blood in proportion to the metal concentration of the maternal blood. In the amniotic fluid, indium concentrations remained below the detection limit. In rats, indium chloride produced dose-dependent maternal toxic effects, with a dose of 400 mg/kg inducing embryotoxicity (embryolethality) and teratogenicity. Doses of 200 and 100 mg/kg were embryotoxic (retarding) and teratogenic, causing skeletal and visceral anomalies in addition to external anomalies (rudimentary or missing tail, syndactylia, clubfoot, exencephalia) in rats. In rabbits, 200 mg/kg indium chloride was lethal for the dams and the embryos (some of the animals died, and the number of abortions and full resorptions increased). This dose was found to be teratogenic (caused gross renal anomalies) and increased the frequency of fetuses with skeletal retardation. In rats, the effects of indium chloride causing fetal retardation was found to be independent of exposure time. The teratogenic effects were the highest on d 11 and 12 of gestation, when indium chloride caused gross external malformations. Data suggest that the teratogenic effects of indium chloride can be attributed primarily to a direct cytotoxic action of indium resulting from placental transfer, but the effect is not a selective one, as it appears only in the presence of maternal toxic effects.

The Effects of Carbon Disulfide and Ethanol on the Circulatory System of Rats

Carbon disulfide exerted adverse effects on the structure or hemodynamics of the cardiovascular system, and whether ethanol exposure modifies the cardiovascular effect of carbon disulfide, was examined. Male Sprague-Dawley rats were used in the study. Animals in the control and ethanol groups drank water containing 5% sugar, or 10% ethanol in addition to 5% sugar, respectively, for 14 wk. Sepatare animals inhaled 700 mg/m3 of carbon disulfide for 6 h daily. Carbon disulfide treatment did not affect the food and fluid consumption of the animals, while this gas decreased body mass gain. CS2 increased arterial blood pressure and cardiac index, decreased their cardiac output, the fraction of the cardiac output, and blood flow for the kidneys and the lungs, and increased the relative heart, liver, and kidneys mass and the vascular resistance of the brain, lungs, and kidneys. Ethanol decreased the food and fluid consumption and body mass gain of the animals, the fraction of the cardiac output for the kidneys, and the vascular resistance of the liver, while it increased the blood flow of the brain and liver. Simultaneous administration of carbon disulfide and ethanol decreased the heart rate and increased the QRS duration. Significant interaction was found between the effect in case of heart rate, PQ distance, and QRS duration; carbon disulfide significantly increased the minimal–moderate effect of ethanol on all three parameters. With histological examinations no pathologic alterations were found in the organs studied. It was concluded that the early hemodynamic changes produced by carbon disulfide may play a significant role in the pathomechanism of the effects of the substance (hypertension, damage to the myocardium and kidneys). On the other hand, a potentiating interaction of carbon disulfide was expected with the effects of ethanol, at the administered concentration and dose in the study.

Effects of simultaneous alcohol and toluene poisoning on the cardiovascular system of rats.

A group of rats was fed a control liquid diet while another group was fed a liquid diet containing alcohol up to 36% of the total caloric intake. One-half of both groups was placed in an inhalation chamber supplied with fresh air; the other two half-groups were made to inhale air containing 4000 mg toluene/m3 for 6 hr daily, 5 days a week, for a period of 4 weeks. After exposure the ECG, hematocrit, histological structure of the heart, blood pressure, cardiac output, distribution of the cardiac output to the organs, nutritive blood flow and circulatory resistance of the organs were studied in these groups of animals. The ECG, hematocrit values, and histological and histochemical structure of the heart did not change in any of the groups. Toluene inhalation increased myocardial vascular resistance and reduced cerebral nutritive blood flow. Alcohol ingestion reduced the arterial blood pressure, the cardiac index, and nutritive blood flow to the myocardium, kidney, skin, and carcass, while myocardial and cutaneous vascular resistance as well as the cerebral blood flow fraction increased. It was concluded that the cardiovascular sites of action of toluene and alcohol were not identical.

Hemodynamic effect of indium chloride in pregnant rats.

Daily indium chloride doses of control (0) or 200 mg/kg were administered orally to pregnant Sprague-Dawley (SD) rats by gavage, on d 6-15 of gestation. On d 16 of gestation hemodynamic tests were performed; Arterial blood pressure, cardiac output (CO), and volume organ blood flow were determined with radioactive microspheres using the reference sample method (McDevitt & Nies, 1976). Indium chloride increased the cardiac index (CI), but did not change arterial blood pressure and total peripheral resistance (TPR). Indium decreased the organ fractions of the cardiac output to kidneys, ovaries, uterus, and placenta, while those to brain, lungs, and liver were not affected. In the placenta the blood flow was reduced significantly while the vascular resistance increased. The blood flow and vascular resistance did not change in the rest of the organs studied. The changes in arterial blood pressure, CO, CI, TPR, organ fraction of cardiac output, blood flow, and vascular resistance in most of the organs displayed normal responsiveness to noradrenaline (NA) infusion. The reduction of uterine and placenta fractions and placental blood flow, produced by NA infusion were significantly greater in control than in the indium-treated group. Data indicate that the hemodynamic changes induced by indium are detrimental to the fetus. Indium chloride exposure modifies the maternal effect of noradrenaline such that there is maternal survival at the expense of fetal mortality.

Roma és nem roma munkanélküliek közegé szségügyi helyzete az Ózdi kistérségben

INTRODUCTION In their previous studies authors of the present work showed that public health situation and socioeconomic position of unemployed Roma persons in the Ozd microregion were well below the average of the whole Hungarian population. AIM To continue these previous studies, the authors wanted to determine whether the greater proportion of the unemployed Roma persons in the Ózd microregion compared to the country average could contribute to the worse public health situation and the poor hygienic situation of the living environment of unemployed persons hardly or not suitable for learning. METHOD Data from 400 unemployed Roma (96 males, 97 females) and caucasian non-Roma subjects (114 males, 93 females) obtained in 2012 and 2013 using self-completed and interview questionnaires were analysed. In addition, occupational medical examination methods were applied and the results were analysed. RESULTS It was found that all studied parameters (public health and epidemiological safety, in-door living environmental hygiene, conditions for learning) indicated significantly disadvantaged situation of the Roma compared to the non-Roma unemployed persons. There was a clear relationship between these examined parameters and other factors adversely influencing the quality of life of Roma persons (unemployment, deep poverty, lower level of education, shorter life span). Finally, the results showed that the quality of life conditions of the lowest 1/10, 1/5 of the non-Roma unemployed persons were comparable to those found in the average of Roma unemployed persons. CONCLUSIONS The authors conclude that i) the greater proportion of unemployed Roma persons in the Ozd microregion contributes to the worse public health, epidemiological safety and the worse living conditions of unemployed persons in this regions of the country; ii) It would be essential to provide Roma persons with conditions appropriate for learning, which could enable them to get qualification necessary for employment.

Roma és nem roma munkanélküliek közegészségügyi helyzete az Ózdi kistérségben@@@Public health issues of Roma and non-Roma unemployed persons in the Ózd microregion

INTRODUCTION In their previous studies authors of the present work showed that public health situation and socioeconomic position of unemployed Roma persons in the Ozd microregion were well below the average of the whole Hungarian population. AIM To continue these previous studies, the authors wanted to determine whether the greater proportion of the unemployed Roma persons in the Ózd microregion compared to the country average could contribute to the worse public health situation and the poor hygienic situation of the living environment of unemployed persons hardly or not suitable for learning. METHOD Data from 400 unemployed Roma (96 males, 97 females) and caucasian non-Roma subjects (114 males, 93 females) obtained in 2012 and 2013 using self-completed and interview questionnaires were analysed. In addition, occupational medical examination methods were applied and the results were analysed. RESULTS It was found that all studied parameters (public health and epidemiological safety, in-door living environmental hygiene, conditions for learning) indicated significantly disadvantaged situation of the Roma compared to the non-Roma unemployed persons. There was a clear relationship between these examined parameters and other factors adversely influencing the quality of life of Roma persons (unemployment, deep poverty, lower level of education, shorter life span). Finally, the results showed that the quality of life conditions of the lowest 1/10, 1/5 of the non-Roma unemployed persons were comparable to those found in the average of Roma unemployed persons. CONCLUSIONS The authors conclude that i) the greater proportion of unemployed Roma persons in the Ozd microregion contributes to the worse public health, epidemiological safety and the worse living conditions of unemployed persons in this regions of the country; ii) It would be essential to provide Roma persons with conditions appropriate for learning, which could enable them to get qualification necessary for employment.

Public health issues of Roma and non-Roma unemployed persons in the Ózd microregion

INTRODUCTION In their previous studies authors of the present work showed that public health situation and socioeconomic position of unemployed Roma persons in the Ozd microregion were well below the average of the whole Hungarian population. AIM To continue these previous studies, the authors wanted to determine whether the greater proportion of the unemployed Roma persons in the Ózd microregion compared to the country average could contribute to the worse public health situation and the poor hygienic situation of the living environment of unemployed persons hardly or not suitable for learning. METHOD Data from 400 unemployed Roma (96 males, 97 females) and caucasian non-Roma subjects (114 males, 93 females) obtained in 2012 and 2013 using self-completed and interview questionnaires were analysed. In addition, occupational medical examination methods were applied and the results were analysed. RESULTS It was found that all studied parameters (public health and epidemiological safety, in-door living environmental hygiene, conditions for learning) indicated significantly disadvantaged situation of the Roma compared to the non-Roma unemployed persons. There was a clear relationship between these examined parameters and other factors adversely influencing the quality of life of Roma persons (unemployment, deep poverty, lower level of education, shorter life span). Finally, the results showed that the quality of life conditions of the lowest 1/10, 1/5 of the non-Roma unemployed persons were comparable to those found in the average of Roma unemployed persons. CONCLUSIONS The authors conclude that i) the greater proportion of unemployed Roma persons in the Ozd microregion contributes to the worse public health, epidemiological safety and the worse living conditions of unemployed persons in this regions of the country; ii) It would be essential to provide Roma persons with conditions appropriate for learning, which could enable them to get qualification necessary for employment.