Véronique Alunni-Perret

Scanning Electron Microscopy Analysis of Experimental Bone Hacking Trauma

The authors report on their macro- and microscopy study of bone lesions made by a sharp force instrument (a single blade knife), and a sharp-blunt instrument classified as a chopping weapon (a hatchet). The aim of this work was to attempt to identify the instrument by analyzing the general class characteristics of the cuts. Each weapon was used on human bones. The results indicate that macroscopic analysis is more problematic. The microscopic analysis assessed that characteristics examined were effective in distinguishing sharp from sharp-blunt injury to the bone. The microscope facilitates analysis unachievable with macroscopic methods, some three-dimensional characteristics not visible to the naked eye being clearly defined with its use. Emphasis has been placed on the value of SEM as an anthropologists tool in bone lesion injuries.

Aristolochic acid (AA)-DNA adduct as marker of AA exposure and risk factor for AA nephropathy-associated cancer.

Volker M. ARLT*, Véronique ALUNNI-PERRET, Gérald QUATREHOMME, Patrick OHAYON, Laetitia ALBANO, Hacène GAı̈D, Jean-Francois MICHIELS, ALAIN MEYRIER, Elisabeth CASSUTO, Manfred WIESSLER, Heinz H. SCHMEISER and Jean-Pierre COSYNS Section of Molecular Carcinogenesis, Institute of Cancer Research, Sutton, United Kingdom Department of Clinical Forensic Medicine and Forensic Pathology, Hôpital Pasteur, Nice, France Department of Nephrology, Hôpital Pasteur, Nice, France Department of Pathology, Hôpital Pasteur, Nice, France Department of Nephrology, Hôpital Européen Georges Pompidou, Paris, France Division of Molecular Toxicology, German Cancer Research Center, Heidelberg, Germany Department of Pathology, UniversitéCatholique de Louvain Medical School, Brussels, Belgium

A comparison between neural network and other metric methods to determine sex from the upper femur in a modern French population.

Forensic anthropologists are frequently asked to assess partial or badly damaged skeletal remains. One such request led us to compare the predictive accuracy of different mathematical methods using four non-standard measurements of the proximal femur (trochanter-diaphysis distance (TD), greater-lesser trochanter distance (TT), greater trochanter width (TW) and trochanter-head distance (TH)). These measurements were taken on 76 femurs (38 males and 38 females) of French individuals. Intra- and inter-observer trials did not reveal any significant statistical differences. The predictive accuracy of three models built using linear and non-linear modelling techniques was compared: discriminant analysis, logistic regression and neural network. The neural network outperformed discriminant analysis and, to a lesser extent, logistic regression. Indeed, the best results were obtained with a neural network that correctly classified 93.4% of femurs, with similar results in males (92.1%) and females (94.7%). Univariate functions were less accurate (68-88%). Discriminant analysis and logistic regression, both using all four variables, led to slightly better results (88.2% and 89.5%, respectively). In addition, all the models, save the neural network, led to unbalanced results between males and females. In conclusion, the artificial neural network is a powerful classification technique that may improve the accuracy rate of sex determination models for skeletal remains.

Assessment of the accuracy of three-dimensional manual craniofacial reconstruction: a series of 25 controlled cases

The aim of this work was to estimate the accuracy of craniofacial reconstruction (CFR), from a series of 25 controlled cases. Three protocols of blind CFRs (exhibiting an increasing complexity from A to C) were assessed in this paper, allowing comparison of the CFR with the actual face of the deceased. The whole results showed that an excellent, or good, to middle resemblance (between the blind CFR and the actual face of the subject) was reached in 9 out of 25 cases, but the success gradually increased from the A to the C protocol of CFR, reaching six cases out of eight in the latter. Statistical comparison of measurements (between the blind CFR and the actual face) was also achieved, revealing that some anthropological distances were constantly underestimated or overestimated. This experiment shows that a thorough anthropological, odontological, and X-ray analysis is indispensable before performing a CFR, and these encouraging results justify further efforts of research in this field.

Determination of heroin after embalmment

A 30-year-old male died in Thailand after a scuffle. The corpse was embalmed and repatriated to France where an autopsy was performed. As usual in cases of embalmment, fluids such as blood and urine were unavailable and the toxicological analyses was performed on the bile and the liver. An overdose of heroin was determined as the cause of death. A review of the literature indicates that several drugs can be detected in fluids and tissues that contain formaldehyde. This case demonstrates that in embalmed corpses, toxicological assessment is still possible, e.g. after heroin fatalities.

Fatal cerebral malaria diagnosed after death in a French patient

We report on the case of a French citizen who was found dead in his home, 4 days after returning from Cameroon. The patient died of imported malaria, as revealed by the postmortem investigations. Few such cases have been reported throughout the world. This article reviews deaths due to malaria diagnosed at the time of autopsy in France between 1995 and 2005. We conclude that the nonspecific symptoms of malaria can lead to a misdiagnosis and the need for a forensic expert to intervene at the scene of death, which usually occurs in the home. We will remind forensic pathologists of the clinical, biologic, and forensic aspects of this infectious disease. In particular, the uses of microbiologic analyses, the QBC malaria test and the Core malaria Pan/Pv/pf test as well as brain tissue histology will be reviewed.

Scanning electron microscopy analysis of experimental bone hacking trauma of the mandible.

The authors report on a macroscopic and microscopic study of human mandible bone lesions achieved by a single-blade knife and a hatchet. The aim of this work was to complete the previous data (scanning electron microscopy analysis of bone lesions made by a single-blade knife and a hatchet, on human femurs) and to compare the lesions of the femur with those of the mandible. The results indicate that the mandible is a more fragile bone, but the features observed on the mandible are quite similar to those previously observed on the femur. This work spells out the main scanning electron microscopy characteristics of sharp (bone cutting) and blunt (exerting a pressure on the bone) mechanisms on human bone. Weapon characteristics serve to explain all of these features.

Acute fatal poisoning with cyamemazine

A case of fatal poisoning with cyamemazine is presented. The cyamemazine was identified in post-mortem blood using a specific gas chromatographic/mass spectrometry method. The autopsy blood concentration of cyamemazine was 1800 ng/ml. Chronic use of cyamemazine was demonstrated by the presence of the drug in hair. Two other drugs were also detected (bromazepam and trimeprazine). We think that this current blood concentration (1800 ng/ml) is a fatal blood concentration because of the negativity of the other parameters, but careful interpretation of analytical findings are important, the possibility that this death was a consequence of the toxicity of combined drugs could not be excluded. Not many therapeutics and toxic levels were previously reported in overdosage cases in which cyamemazine was involved. We consider that this concentration is only of guidance value for a fatal cyamemazine poisoning.