Veruscka Mannoni
Istituto Superiore di Sanità
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Featured researches published by Veruscka Mannoni.
International Journal of Food Microbiology | 2003
Paolo Aureli; Anna Maria Ferrini; Veruscka Mannoni; Snjezana Hodzic; Christina Wedell-Weergaard; Brunello Oliva
The susceptibility of 148 strains of Listeria monocytogenes isolated from food to antibiotics currently used in veterinary and human therapy was determined by standard agar dilution and disk diffusion methods. The antibiotics included amikacin, amoxicillin, cefazolin, chloramphenicol, erythromycin, flumequine, fosfomycin, gentamicin, kanamycin, lincomycin, oxytetracycline, rifampicin, spiramycin, streptomycin, tetracycline, tobramycin and vancomycin. Soussys breakpoints and MIC(50)-MIC(90) values were used to classify the strains into sensitive, moderately sensitive and resistant groups. This work is part of a wider surveillance program on listeriosis started in Italy in 1995.
Journal of Food Protection | 1999
Giovanna Franciosa; Manoocheher Pourshaban; Monica Virginia Gianfranceschi; Antonietta Gattuso; Lucia Fenicia; Anna Maria Ferrini; Veruscka Mannoni; Gregorio De Luca; Paolo Aureli
A total of 1,017 mascarpone cheese samples, collected at retail, were analyzed for Clostridium botulinum spores and toxin, aerobic mesophilic spore counts, as well as pH, a(w) (water activity), and Eh (oxidation-reduction potential). In addition 260 samples from other dairy products were also analyzed for spores and botulinum toxin. Experiments were carried out on naturally and artificially contaminated mascarpone to investigate the influence of different temperature conditions on toxin production by C. botulinum. Three hundred and thirty-one samples (32.5%) of mascarpone were positive for botulinal spores, and 7 (0.8%) of the 878 samples produced at the plant involved in an outbreak of foodborne botulism also contained toxin type A. The chemical-physical parameters (pH, a(w), Eh) of all samples were compatible with C. botulinum growth and toxinogenesis. Of the other milk products, 2.7% were positive for C. botulinum spores. Growth and toxin formation occurred in naturally and experimentally contaminated mascarpone samples after 3 and 4 days of incubation at 28 degrees C, respectively.
Journal of Agricultural and Food Chemistry | 2008
Anna Maria Ferrini; Veruscka Mannoni; Graziella Carpico; Guido Enrico Pellegrini
A novel application of a hybrid biosensor is here employed as an analytical method for the detection and presumptive identification of beta-lactam residues in milk. The method is based on measurements of carbon dioxide (CO2), the production of which is related to the microbial growth of the test microorganism Bacillus stearothermophilus var. calidolactis. The presence of beta-lactams in milk inhibits microbial growth and, consequently, the CO2 production rate. The analysis is based on the variation of CO2 between a milk sample spiked with beta-lactams and a twin milk sample containing beta-lactams plus a broad spectrum beta-lactamase, using an electrochemical device of biosensor. A blank milk sample is included as control. The result is obtained starting from the first 120 min. Moreover, the ability to recognize all of the beta-lactams speeds the total time of analysis when chemical identification and quantification are required. The analytical method appears to be adequate for milk control for qualitative screening purposes, complying with the requirements stated in Decision 2002/657/EC.
Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment | 2006
Anna Maria Ferrini; Veruscka Mannoni; Paolo Aureli
This paper proposes an improved high throughput microbial method for the simultaneous performance of first and second level screening for antibacterial residues in meat. It is based on growth inhibition of B. subtilis on agar medium pH 6, 7.2 and 8, of B. cereus on agar medium pH 5.9, of M. luteus on agar medium pH 8 and of E. coli on agar medium pH 7.2 (research or first level screening) and on the use of confirmatory solutions (Pase, Paba, MgSO4) for the identification or second level screening. In kidney control samples, dialysis membranes were interposed between samples and the agar surface to both prevent the action of lysozyme and reduce false positive results. The proposed method detects β-lactams, sulfonamides, tetracyclines, aminoglycosides, macrolides and quinolones at MRL concentrations and reliably indicates the inhibitor family. Results are obtained in 18–24 h.
Food Control | 1996
Paolo Aureli; Anna Maria Ferrini; Veruscka Mannoni
Abstract A microbial procedure is described for the presumptive identification of some antibiotic and sulphonamide residues in milk. Penicillins, cephalosporins, sulphonamides and streptomycin residues are tested using three different agar media and two microorganisms. The identification is reached by enclosing in the media four different substances to reverse the normal action of the antibiotics and sulphonamides under investigation.
Journal of Medical Microbiology | 2002
Manoocheher Pourshaban; Anna Maria Ferrini; Veruscka Mannoni; Brunello Oliva; Paolo Aureli
Mechanisms of tetracycline resistance were investigated in two recent Listeria monocytogenes isolates from food, with L. innocua 52P tet(r) as a control. Tetracycline resistance was transferred conjugatively from all three strains to L. ivanovii and from one isolate and the control to Enterococcus faecalis. Molecular analysis demonstrated a chromosomal location for the tet determinant, which was identified as tetM in all cases. These studies are the first to show that L. monocytogenes from food could be a source of tetracycline resistance genes able to spread to other micro-organisms.
Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment | 2017
Veruscka Mannoni; Giorgio Padula; Oronzo Panico; A. Maggio; Claudio Arena; M. R. Milana
ABSTRACT The migration of formaldehyde and melamine monomers has been measured on 90 samples of plastic tableware in three different situations – new articles, already used articles and artificially aged articles – by using simulant, contact times and temperatures prescribed by Commission Regulation (EU) No. 10/2011. Formaldehyde was determined by ultraviolet spectroscopy analysis of the coloured complex obtained by reaction with chromotropic acid. Melamine was measured by an ultra high performance liquid chromatography method. Fourier Transformed – Infrared Analysis was applied to characterise the plastic. The results highlighted the presence of different amino resins based on formaldehyde-melamine, urea-formaldehyde or melamine-urea-formaldehyde with different migration behaviour. The migration of monomers was related to progressive degradation of the resins. Ageing studies demonstrated that the potential degradation of the resins and the consequent migration of the monomers may continue throughout the service life of the product. The specific migration limit (SML) of melamine was exceeded after ageing.
Journal of Agricultural and Food Chemistry | 2010
Anna Maria Ferrini; Simona Trenta; Veruscka Mannoni; Remo Rosati; Ettore Coni
Although goat milk production represents today a very small percentage of the world milk market, this percentage has been growing continuously during the past 20 years. Goat milk is the basic milk supply in many developing countries and provides tasteful derivative products in developed countries. Goats, as well as all milk-producing animals, can be affected by mastitis, but goats being considered a minor species, few drugs are specifically registered for these animals; most, at least for mastitis treatment, are usually tested and registered for use in cows. This situation leads often to the adoption for goat milk of withdrawal periods defined for cows even if these extrapolations prove almost never valid for goats. In the present study, the elimination of the β-lactam antibacterial agent ampicillin in goat milk was investigated. Ampicillin was chosen because it is one of the most common antibiotics used by goat farmers against mastitis due to the fact that it is well tolerated and has short elimination times in cows. Goats were treated with long-acting ampicillin at 15 mg (kg of body weight)(-1) by double intramuscular injection at 72 h interval. Milk was collected in a 12 h milking scheme. The method used to determine the levels of ampicillin in goat milk was based on a liquid-liquid extraction of this drug from the matrix, successive derivatization with formaldehyde, and final separation by HPLC with fluorescence detection. The results point out a slow depletion of ampicillin and, consequently, a withdrawal period (13 milkings) longer than that extrapolated and authorized for cows and sheep.
European Journal of Epidemiology | 2002
Lucia Fenicia; Anna Maria Ferrini; Fabrizio Anniballi; Veruscka Mannoni; Paolo Aureli
In Italy, neurotoxigenic Clostridium butyricum has been reported as a new agent of intestinal toxemia botulism, and most of the cases have been associated with enterocolitis. Although infections concomitant with botulism must be treated with antibiotics, this can increase the severity of botulism. We discuss the sensitivity of this agent to certain antibiotics, compared to findings on the sensitivity of C. botulinum.
Archive | 2017
Gaetano Settimo; Marco Gola; Veruscka Mannoni; Marco De Felice; Giorgio Padula; Ambra Mele; Barbara Tolino; Stefano Capolongo
Introduction. The Indoor Air Quality is a topic that in recent years is obtaining great attention in healthcare facilities. Several studies are reporting a growing number of data and research works that allow to know all the possible indoor levels and the development of management procedures (such as materials’ choice, products for cleaning and disinfection, improvement of HVAC system, etc.) in order to improve the healing environments and health of medical and technical staffs, patients, ambulatory users and visitors. Ensuring a good IAQ in hospitals is fundamentally because there are the most vulnerable category of the population for their health conditions; although the assessment of IAQ results arduous.