Vicky Fachinger

The effect of vaccination against porcine circovirus type 2 in pigs suffering from porcine respiratory disease complex

A field study was conducted to investigate the effect of vaccination against porcine circovirus type 2 (PCV2) in pigs suffering from porcine respiratory disease complex (PRDC). A total of 1542 pigs were allocated randomly into two treatment groups at approximately 20 days of age. Groups received either a Baculovirus-expressed recombinant PCV2 Open Reading Frame (ORF) 2 vaccine or placebo by single intramuscular injection. Median onset of PCV2 viraemia and respiratory signs occurred when animals were 18 weeks old. Vaccination reduced the mean PCV2 viral load by 55-83% (p < 0.0001) and the mean duration of viraemia by 50% (p < 0.0001). During the period of study (from 3 to 25 weeks of age) vaccinated animals exhibited a reduced mortality rate (6.63% vs. 8.67%, difference -2.04%; p = 0.1507), an improved average daily weight gain (649 g/day vs. 667 g/day; difference +18 g/day; p < 0.0001) and a reduced time to market (164.8 days vs. 170.4 days; difference -5.6 days; p < 0.0001). The effects on performance were greatest in the 8-week period between the onset of PCV2 viraemia and the end of finishing. These data demonstrate that vaccination against PCV2 alone can significantly improve the overall growth performance of pigs in a multi-factorial, late occurring disease complex such as PRDC.

Reduction of PMWS-associated clinical signs and co-infections by vaccination against PCV2

The effects of a single-dose recombinant Porcine circovirus type 2 (PCV2) open reading frame 2 (ORF2) subunit vaccine were studied in a post-weaning multisystemic wasting syndrome (PMWS)-affected pig herd. A total of 1519 3-week-old piglets were allocated randomly into two treatment groups and either vaccinated against PCV2 or treated with a placebo. Study animals were followed from the time of vaccination until the end of finishing. Onset of PCV2 viraemia and clinical signs of PMWS (wasting, cough, dyspnoea, pallor and lethargy) were observed when animals were approximately 9-10 weeks old. Compared to placebo-treated animals, vaccinated animals had a significantly reduced PCV2 viral load and duration of viraemia (p < 0.0001). This reduction in viraemia was not affected by the level of maternal anti-PCV2 antibodies present at the time of vaccination. During the period of viraemia (10-26 weeks of age) vaccinated animals exhibited a 53% reduction in mortality rate (p = 0.0010), a 4.84 kg higher body weight gain (p < 0.0001) and a significant reduction in clinical signs (p < or = 0.0004). Furthermore, lung samples of vaccinated animals had a considerably reduced number of co-infections with PRRSV and Mycoplasma hyorhinis than lung samples of placebo-treated animals. These data indicate that vaccination against PCV2 alone protects pigs from clinical signs and co-infections associated with PMWS.

T-helper cells from naive to committed

T-helper cells play a central role in the onset and regulation of the antigen-specific immune response. In swine, two subpopulations of CD4 positive T-helper cells could be defined in extra-thymic compartments. Both differ in the surface antigen expression of distinct antigens. Besides the CD4(+)CD8(-) T-helper cell subpopulation representing the phenotype of T-helper cells known from other species, in swine CD4(+) T-helper cells exist showing expression of CD8alpha and MHCII molecules.In primary in vitro immune reactions after simulation with SEB the main response is distributed to the CD4(+)CD8(-) T-cell subpopulation, which contains the majority of naive T-helper cells. During the immune response CD8alpha as well as MHCII molecules are expressed on the surface of activated T-helper cells. This in vitro maturation is combined with a down-regulation of CD45RC. Thus, activated T-helper cells represent a cell phenotype with high similarity to the second porcine T-helper cell subpopulation. This CD4(+)CD8alpha(+) subpopulation contains in its majority MHCII(+) and CD45RC(-) cells. In a secondary in vitro immune response against classical swine fever virus only the CD4(+)CD8(+) T-helper subpopulation is able to respond. Therefore, T-helper memory cells can be distributed to this T-lymphocyte subpopulation. In summary, naive CD4(+)CD8(-)MHCII(-) porcine T-helper cells show an extra-thymic maturation to committed CD4(+)CD8alpha(+)MHCII(+) T-helper cells.

Poxvirus-Induced Immunostimulating Effects on Porcine Leukocytes

ABSTRACT The prophylactic application of inactivated parapox ovis viruses (Baypamun; Bayer AG, Leverkusen, Germany) has been shown to reduce efficiently the outbreak of stress-mediated diseases in different species. However, little is known about the basic mechanism behind this observed stimulatory property. We therefore tested eight inactivated poxvirus strains belonging to three different genera (Orthopoxvirus, Avipoxvirus, andParapoxvirus) for their capacity to activate cells of the porcine innate and specific immune systems in vitro. The results indicated that poxviruses failed to induce increased phagocytosis, oxidative burst, or natural killer cell activity in swine. In contrast, enhanced release of interleukin-2, alpha interferon, and gamma interferon, as well as strong proliferation, could be measured. Flow cytometric analyses and cell sorting experiments identified T-helper cells as the main target responding to inactivated poxviruses: the activated cells had a CD4high CD25+ major histocompatibility complex type II-positive phenotype and were the major source of secreted cytokines. Together, the results demonstrated that all tested poxviruses possessed immunostimulating capacity. These in vitro poxvirus-induced effects may be responsible at least in part for the in vivo immunostimulating capacity of inactivated poxviruses.

Influence of age on the effectiveness of PCV2 vaccination in piglets with high levels of maternally derived antibodies

Two field studies were conducted to investigate the influence of age on the efficacy of vaccination against Porcine Circovirus Diseases (PCVD) in animals with high levels of maternally derived antibodies (MDA). A total of 416 piglets (Study 1) and 600 piglets (Study 2) were randomly allocated to one of three groups. Two groups in each study received a single dose of a PCV2 subunit vaccine, one group at 1 week old and the other at 3 weeks of age. The third group was left untreated. Animals vaccinated at 3 weeks of age showed a significantly higher average daily weight gain and significantly reduced viraemia following PCV2 infection than the respective control groups. This difference was not observed in pigs vaccinated at 1 week of age. Furthermore, only animals vaccinated at 3 weeks of age showed an increased serological response and a higher frequency of IgM-positive animals compared with controls. The data indicated that PCV2 vaccination in the presence of high MDA levels is efficacious when used in 3-week old but not in 1-week old pigs. As the range of MDA titres of pigs vaccinated at both 1 and 3 weeks of age were comparable, the data suggest that PCV2 vaccine efficacy was independent of the level of MDA. It appears that other age-related factors affecting the active and passive transfer of immunity may perhaps have interfered with the efficacy of the vaccine in 1-week old piglets. These findings have implications for future PCV2 vaccine testing and administration strategies.

Evidence for a parapox ovis virus‐associated superantigen

As shown in a number of species, susceptibility to infectious diseases can be efficiently reduced following application of inactivated parapox ovis viruses (iPPOV). However, the basic mechanism for this stimulating capacity of iPPOV remains unclear. When analyzed, the interaction of iPPOV with porcine peripheral blood mononuclear cells was seen to involve T helper cells as the main target cell population responding to iPPOV. These cells displayed a strong proliferation, and were the major source for the observed increased levels of IL‐2. Activation of the T helper cells was MHC class II dependent, but not MHC class II restricted: cellular processing of iPPOV was not required for presentation by autologous, allogeneic or xenogeneic MHC class II molecules. Furthermore, CD3 and CD4 molecules were involved in the stimulation, indicating a receptor‐mediated activation of T helper cells. The results demonstrated typical characteristics of a superantigen‐induced response providing evidence for a viral component within PPOV functioning as superantigen(s) in swine.

Respiratory function and pulmonary lesions in pigs infected with porcine reproductive and respiratory syndrome virus

Abstract Pulmonary dysfunction was evaluated in pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV, isolate VR-2332) and compared to clinical and pathological findings. Infected pigs developed fever, reduced appetite, respiratory distress and dullness at 9days post-inoculation (dpi). Non-invasive pulmonary function tests using impulse oscillometry and rebreathing of test gases (He, CO) revealed peripheral airway obstruction, reduced lung compliance and reduced lung CO-transfer factor. PRRSV-induced pulmonary dysfunction was most marked at 9–18 dpi and was accompanied by a significantly increased respiratory rate and decreased tidal volume. Expiration was affected more than inspiration. On histopathological examination, multifocal areas of interstitial pneumonia (more severe and extensive at 10 dpi than 21 dpi) were identified as a possible structural basis for reduced lung compliance and gas exchange disturbances.