Victoria Jato

Variation assessment of airborne Alternaria and Cladosporium spores at different bioclimatical conditions.

The study of mould spores is of major importance as many fungi can cause considerable economic losses worldwide acting as plant pathogens or triggering respiratory diseases and allergenic processes in humans. Knowledge of spore production relationships to different altitudes or weather patterns can be applied in a more efficient and reliable use of pesticides or improving diagnosis and treatment of respiratory allergic diseases. In this way monitoring of Cladosporium cladosporioides, C. herbarum and Alternaria spp. airborne spores during 2002 was carried out by means of three LANZONI VPPS 2000 pollen traps located in areas of north-west Spain at various altitudes and with various weather patterns. High spore counts were recorded in the late summer and early autumn, with a fairly similar hourly spore-count pattern, increasing the concentrations in the late evening (7-10 p.m.). High spore concentrations were detected in inland rural areas in front of coastal ones. As the continentality index increased, C. cladosporioides spore concentrations rose and Alternaria declined. C. herbarum concentrations increased with increasing height above sea level. The weather factor displaying the strongest positive correlation with mean daily spore counts was temperature. The optimal conditions for high airborne spore concentrations were recorded at temperatures ranging from 23-29 degrees C and RH values of around 80%, followed rapidly by rainfall in Vigo and Ourense and preceded by heavy rain two days prior to recording peak values in Trives.

Pollen content in the atmosphere of Lugo (NW Spain) with reference to meteorological factors (1999–2001)

This study shows the results of monitoring thepollen present in the atmosphere of the city ofLugo for three years (1999–2001) using a Hirstvolumetric trap (model Lanzoni VPPS-2000).During the three-year study, 61,381 pollengrains were counted. Poaceae represents 39.5%of the total pollen identified, Pinus12.3%, Quercus 8.7% and Betula8.4%. More than half of the total annualpollen was recorded in June and July. Itscorrelation was positive with temperature,hours of sun and wind speed, and negative withrainfall and relative humidity. The maximumvalues were recorded between 11 am and 8 pm,coinciding with the highest temperatures andlowest relative humidity.

Heat and chill requirements of Fraxinus flowering in Galicia (NW Spain)

Fraxinus pollen data from eight Galician localities (1999-2003), recorded using 7-day Lanzoni VPPS pollen traps, were studied to determine their temporal and spatial distribution. The determination of the chill and heat required to trigger flowering and the start cumulative date were calculated using ten years of pollen data from Ourense. The sum of maximum temperatures from the 55 days before the peak date showed the lowest standard deviation coefficient and the mean quantity of accumulated heat was 741. Temperatures below 0°C and/or rainfall at the beginning of flowering caused a longer period before the peak date was registered. Temperatures recorded in November were very important for chill accumulation and determine the heat requirement needed to trigger Fraxinus flowering in Galicia.

Seasonal variation of airborne fungal spore concentrations in a vineyard of North-West Spain

Conidial types collected daily in the air above a vineyard in northwest Spain were identified and counted. A total of 26 fungal spore types were recognised; ten of which (Cladosporium, Botrytis, Fusarium-Leptosphaeria type,Torula, Puccinia, Alternaria, Uncinula, Helminthosporium type,Agrocybe andStemphylium) gave a seasonal total concentration exceeding 1000 spores. Seasonal patterns are shown for 12 of the identified taxa.

Influence of precipitation and temperature on airborne pollen concentration in Santiago de Compostela (Spain)

This paper presents data of airborne pollen concentration of Pinus , Platanus , Quercus , Betula , Poaceae and Castanea , the most abundant pollen types, during the last eight years, in Santiago de Compostela (NW Iberian Peninsula). We determined their respective principal pollen release periods and analysed statistically the influence of the main meteorological parameters on their pollen concentration, obtaining a negative correlation with precipitation and a positive correlation with temperature, in the majority of the cases, during these years. A detailed study of accumulated pollen values, in relation to accumulated average temperatures and the precipitation distribution, enabled us to establish a relationship between these parameters and the principal pollination period of each of these taxa.

The influence of climate changes in Platanus spp. pollination in Spain and Italy

There is evidence of a significant increase in air temperature in the northern hemisphere over recent decades, with consequent changes for anemophilous pollen. In this work we present the effects of climatic change on Platanus spp. pollination in different areas of Italy and Spain, characterized by different climates. In particular, the historical series of pollen monitoring and meteorological data of two Italian stations, Perugia (1982 – 2003) and Torino (1985 – 2003), and two Spanish stations, Santiago de Compostela (1992 – 2003) and Vigo (1994 – 2003), were analysed. The changes recorded in all stations included the timing and behaviour of pollen release. However, no or minimal influence on the total pollen emission was found. Research has linked the changes in phenological events to an increase in temperature, moreover in this study temperature changes are believed to be mainly responsible for the variations recorded in the pollen season of Platanus. A previous start of pollination (−0.66; −1.21; days/year) is reported in both Italian stations where the temperatures have significantly increased and a delay of 0.2 – 0.8 days/year in Spanish stations where a different trend of temperature is recorded. Other important data is given regarding the type of discharge of pollen grains during the pollen season. Pollination curves are examined by two statistical shape parameters (kurtosis and skewness) which show that pollen release is more gradual with higher temperatures or faster under colder conditions. A regression analysis links the atmospheric pollen presence to mean air temperature.

The use of aerobiological methods for forecasting Botrytis spore concentrations in a vineyard

Abstract Grey mould, caused by Botrytis cinerea, is one of the most common diseases in Galician vineyards. Knowledge of local airborne spore concentrations is of great value for developing models to predict fungal propagule concentrations, thus enabling phytosanitary treatments to be applied when a real risk of infection is detected. In order to develop an accurate model for forecasting airborne B. cinerea spore concentrations, a phenological and aerobiological survey was carried out in a vineyard located in Galicia (north-west Spain), from 2004 to 2008. Phenological observations were made on 20 vines of each of three grape varieties (Treixadura, Godello and Loureira) using the BBCH standardised phenological scale. A Hirst-type volumetric spore-trap was used for the aerobiological monitoring. The study sought to chart Botrytis spore concentrations as function of grapevine phenological stage. The highest spore concentrations over the grapevine cycle as a whole were recorded in 2008 (37299 spores), and the lowest in 2005 (1700 spores). In the five study years, the highest concentrations were registered during stage 8 (berry ripening), stage 7 (fruit development) and stage 5 (inflorescence emergence). The weather-related parameters displaying the most significant correlation with spore concentrations were dew point and relative humidity. ARIMA (Autoregressive Integrated Model of Running Mean) time-series models was used to forecast daily spore concentrations, considering B. cinerea spore concentrations and weather data as predictor variables.

The relationship between the flowering phenophase and airborne pollen of Betula in galicia (N.W. spain)

The aim of this work was to investigate thephenological behaviour of Betula in Galicia, NW Spain, and to examine therelationship between the Betula pollencurves and the flowering phenophase. Threetrees were chosen from the each of ninepopulations of Betula located atdifferent altitudes and phytogeographicpositions. Phenological observations of theflowering periods of Betula were made ineach of them. Environmental factors such asfrequency of mist, latitudinal and topographicposition, proximity of the ocean, degree ofsolar exposure, and altitude result inphenological differences between theinvestigated populations. The correlationbetween the Pollinic Production Index of Betula pollen in Galicia and theaerobiological data of the seven monitoringstations showed that the period in which thehighest concentrations were registered wasalmost synchronous with the flowering times atmost of the phenological stations studied.Other factors such as transport and reflotationshould also be taken into account to provide anadequate interpretation of the aerobiologicaldata of Betula pollen in the atmosphere.

Pinus pollen aerobiology and clinical sensitization in northwest Spain

BACKGROUND Pinus pollen allergy has been generally considered to be rare and clinically insignificant. In our geographical area, Pinus pollen constitutes one of the most predominant pollen together with Poaceae and Urticaceae pollen. Pinus pinaster and Pinus radiata are the main species. OBJECTIVE This work aimed to determine the atmospheric fluctuations and the existence of patients monosensitized to Pinus pollen in our region. METHODS Patients attending our outpatient clinic in the last 4 years with positive cutaneous tests to Pinus pollen and with respiratory symptoms were selected. They were skin tested with commercial extracts of a battery of inhalants including Pinus pollen. Serologic specific IgE to Pinus pollen was performed by CAP system (Pharmacia & Upjohn, Uppsala, Sweden). Airborne Pinus pollen counts in our city were obtained for a 5-year period (1995 to 1999). RESULTS We present 10 patients with sensitivity to Pinus pollen and with symptomatology coinciding with Pinus pollen season (February to April). Most of these patients were monosensitizated to Pinus pollen and suffered from seasonal rhinoconjunctivitis. Pinus pollen is present in our area in large amounts from February to April with a peak pollen count in March and April. CONCLUSIONS Pinus pollen may be an important allergen since by itself can be the only cause of allergic disease. Therefore, we think that it must be taken into account in patients living in areas with high Pinus pollen concentrations and with seasonal respiratory disease.

Fungi associated with three houses in Havana (Cuba)

Aerobiological sampling was carried out during two consecutive years, 2000 and 2001, in order to ascertain the level of fungal spores present in indoor and outdoor environments in the city of Havana. The samples were collected using Burkard (Burkard Personal Culture and Burkard Personal Slide) and Andersen traps. Sampling was completed by using adhesive tape. We identified 19 spore types, of which the most frequent were Aspergillus (A. flavus, A. niger and A. clavatus), Penicillium (P. citrinum) and Cladosporium (Cl. cladosporioides) . The other fungal types identified include important potential allergens and/or phytopathogens.