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Dive into the research topics where Vijaya Lakshmi Nag is active.

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Featured researches published by Vijaya Lakshmi Nag.


Journal of the Neurological Sciences | 2008

Dengue encephalopathy in children in Northern India: Clinical features and comparison with non dengue

Sanjeev Tripathi; J.J. Tambe; Vikas Arora; Amit Srivastava; Vijaya Lakshmi Nag

BACKGROUND Acute febrile encephalopathy (AFE) is a common cause of childhood hospital admissions in Lucknow. In recent years, many patients have been hospitalized with AFE and hemorrhagic manifestations, some of whom were proven to have dengue viral infection. OBJECTIVES To (i) define the role of dengue encephalopathy (DE) as a cause of AFE in children in Lucknow, (ii) document features of dengue hemorrhagic fever (DHF) in them and (iii)compare clinical features of definite dengue and non dengue AFE. STUDY DESIGN Prospective study at a teaching hospital in northern India. Children between 1-12 years of age hospitalized with fever and altered consciousness of 2 weeks or less duration were enrolled after excluding bacterial and tuberculous meningitis and frank hepatic encephalopathy. Clinical and laboratory details were charted. Haemagglutination inhibition (HI) test for dengue and Japanese encephalitis viruses in paired sera and IgM antibody capture ELISA for dengue were done. Real time PCR was done in those samples testing positive for dengue IgM. Those with either positive HI test or positive dengue PCR in CSF or serum were considered definite dengue infection and features of DHF were charted in them. Those negative for IgM antibodies after 5 days of illness or whenever done, HI test, were considered definite non dengue. Clinical and laboratory features were compared between definite dengue and non dengue groups. RESULTS A total of 265 patients of AFE were enrolled over a 2 year period. HI test was positive in 15/49 (30.6%) and IgM in 52/238 (21.8%) patients thus tested. A total of 62 patients were positive for dengue antibodies by either test. Real time PCR assay for dengue virus genome was positive in 28/42 (69%) tested -- 21/29 (72.4%) in CSF and 9/15 (60%) in serum. A total of 39 patients met the criteria for definite dengue infection of which only 2 fulfilled the WHO criteria for DHF. Comparing DE and non DE, rash, bleeding, swelling over body, and hepatomegaly were significantly more common and meningeal signs less frequent in DE. Mean platelet counts and serum albumen were lower and liver enzymes and INR were significantly higher in DE. CONCLUSIONS Dengue viral infection is a cause of AFE in children in this region. Majority of DE here appears to be due to viral invasion of brain as suggested by high PCR positivity in CSF and lack of WHO criteria for DHF. Differentiating features of DE include swelling and hepatic dysfunction.


BioScience Trends | 2011

The advantage of using IS6110-PCR vs. BACTEC culture for rapid detection of Mycobacterium tuberculosis from pleural fluid in northern India.

Anand Kumar Maurya; Surya Kant; Ram Awadh Singh Kushwaha; Vijaya Lakshmi Nag; Manoj Kumar; Tapan N. Dhole

Pleural tuberculosis is an extra-pulmonary disease which poses a diagnostic dilemma. The detection of mycobacterial DNA by IS6110 polymerase chain reaction (PCR) in clinical samples is a promising approach for the rapid diagnosis of pleural tuberculosis infections. The aim of the present study is to evaluate the advantage of using IS6110 PCR for rapid detection of Mycobacterium tuberculosis (M. tuberculosis) from pleural fluid. 102 clinically suspected cases of pleural tuberculosis cases were enrolled from inwards and outwards of the Department of Pulmonary Medicine at Chattrapati Shahuji Maharaj Medical University, Lucknow from April 2007 to April 2010. The pleural fluids were processed at the Mycobacteriology Laboratory of Department of Microbiology at Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Pleural fluid samples were processed and examined by Ziehl Neelsen (ZN) staining for acid fast bacilli and detection of M. tuberculosis by BACTEC culture. We applied IS6110 PCR to detect specific M. tuberculosis complex in pleural fluid samples. We found a significant difference in sensitivity of different tests, acid fast bacilli were detected in 17 (16.6%) samples by ZN Staining , 47 (46.1%) by BACTEC culture and using IS6110 PCR, 62 (60.7%) were positive for IS6110 PCR for M. tuberculosis. We found IS6110 PCR was much more sensitive than ZN staining and BACTEC culture. IS6110 PCR detection of M. tuberculosis may be very useful in cases that are highly suspect as pleural tuberculosis and those that are negative for AFB and culture. IS6110 PCR may gain an immense prospective to better clinicians ability to improve diagnosis of pleural tuberculosis.


Case reports in dermatological medicine | 2015

Mixed Cutaneous Infection Caused by Mycobacterium szulgai and Mycobacterium intermedium in a Healthy Adult Female: A Rare Case Report.

Amresh Kumar Singh; Rungmei S. K. Marak; Anand Kumar Maurya; Manaswini Das; Vijaya Lakshmi Nag; Tapan N. Dhole

Nontuberculous mycobacteria (NTMs) are ubiquitous and are being increasingly reported as human opportunistic infection. Cutaneous infection caused by mixed NTM is extremely rare. We encountered the case of a 46-year-old female, who presented with multiple discharging sinuses over the lower anterior abdominal wall (over a previous appendectomy scar) for the past 2 years. Microscopy and culture of the pus discharge were done to isolate and identify the etiological agent. Finally, GenoType Mycobacterium CM/AS assay proved it to be a mixed infection caused by Mycobacterium szulgai and M. intermedium. The patient was advised a combination of rifampicin 600 mg once daily, ethambutol 600 mg once daily, and clarithromycin 500 mg twice daily to be taken along with periodic follow-up based upon clinical response as well as microbiological response. We emphasize that infections by NTM must be considered in the etiology of nonhealing wounds or sinuses, especially at postsurgical sites.


Indian Journal of Orthopaedics | 2014

The efficacy of diagnostic battery in Pott's disease: A prospective study

Manoj Kumar; Raj Kumar; Arun Srivastva; Vijaya Lakshmi Nag; Narendra Krishnani; Anand Kumar Maurya; Tapan N. Dhole; Sunil G. Babu

Background: The diagnosis of Potts disease is mostly based on clinicoradiological observations substantiated by the bacterial culture, staining and histopathology. Since, no single technique is enough to conclude Potts disease in diagnosis, the present study was undertaken to correlate the clinicoradiological, microbiological, histopathological and molecular method to evaluate the effectiveness in diagnosis of Potts disease. Materials and Methods: 62 clinicoradiologically suspected cases of Potts disease were included in this study. The specimens for diagnostic work up were collected either during surgery or by computed tomography guided fine needle aspiration. All these specimens were tested for tuberculosis (TB) through Ziehl-Neelsen (ZN) microscopy, BACTEC culture, histopathology and polymerase chain reaction (PCR). The final diagnosis was established by the results of performed tests and clinicoradiological improvement of cases at the end of 6 months on anti tubercular treatment. Results: Out of 62 cases, 7 were excluded from this study as these were turned out to be neoplastic lesions on histopathology. Amongst remaining 55 cases, the TB was diagnosed in 39 (71%) on histopathology, 37 (67.5%) on PCR, 27 (49%) on BACTEC culture and 20 (36.3%) on ZN microscopy. Ultimately 45 cases were tested as positive and 10 were detected as negative for TB in combination of ZN microscopy, BACTEC culture and histopathology. PCR was positive in 37 of 45 cases and 10/55 cases remained negative. On clinical analysis of these 10 cases, it was noted that these were cases of relapse/poor compliance. The combination of PCR and histopathology was also shown positive for TB in 45 cases. Hence, the PCR showed a fair positive agreement (Κc = 0.63) against the combined results of all performed traditional methods. Conclusions: The combination of PCR and histopathology is a rapid and efficient tool for diagnosis of Potts disease.


International Journal of Infectious Diseases | 2009

Rapid diagnosis and successful drug therapy of primary parotid tuberculosis in the pediatric age group: a case report and brief review of the literature

Vijaya Lakshmi Nag; Jagdeep Singh; Sashikant Srivastava; Isha Tyagi

Isolated parotid tuberculosis is difficult to diagnose in the pediatric age group. Often the problem leads to surgery. We describe the case of a 13-year-old female who presented with right parotid swelling with facial palsy. Computerized tomography, aspiration cytology, PCR differentiation, culture sensitivity, and drug therapy led to complete resolution. A literature review revealed case reports suggesting a trend towards a conservative approach.


Australian Journal of Rural Health | 2009

Need for syphilis screening and counselling in HIV counselling and testing centres: A curtain raiser study from north India

Vijaya Lakshmi Nag; Nihar Ranjan Dash; Ashutosh Pathak; Surendra Kumar Agarwal

BACKGROUND Voluntary counselling and testing centres (VCTC) are important HIV screening points for the population with suspicion or apprehension for HIV, because of high-risk exposures. Theoretically, these are also at the risk of having co-infections, commonest being syphilis. The present short-term study was aimed at knowing the sero-reactivity of syphilis among a study cohort attending the VCTC in King Georges Medical University, Lucknow, India. METHOD During a 2.5-month period, 49 HIV-positive and 171 HIV-negative sera were tested for venereal disease research laboratory (VDRL). The positive sera were further tested for treponema pallidum haemagglutination (TPHA). RESULT Eleven (22.4%) HIV-positive sera and 104 (60.8%) HIV-negative sera were VDRL-reactive (>or=1:8 dilutions). Of these, TPHA was reactive in three (27.3%) and four (3.9%), respectively. CONCLUSION The result might suggest the need for routine screening and counselling for syphilis at VCTC. Studies from other centres on larger population are required.


Biomedical and Biotechnology Research Journal (BBRJ) | 2017

Recent methods for diagnosis of nontuberculous mycobacteria infections: Relevance in clinical practice

Anand Kumar Maurya; Vijaya Lakshmi Nag; Surya Kant; Anuradha Sharma; Ravi Shekhar Gadepalli; Ram Awadh Singh Kushwaha

Nontuberculous mycobacteria (NTM) infections are ever more important in recent years for leading causes of morbidity and mortality worldwide. Clinical appearance of Mycobacterium tuberculosis (TB) complex and NTM is same, but the treatment regimen is always different. NTM is challenging for both diagnostic and therapeutic with reason that it mimic pathological, microbiological, immunological, and radiological findings of TB. Newer molecular diagnostic methods allow for a better identification of NTM infections in patients not responding to antitubercular treatment and falsely categorized as drug-resistant TB. This article will explore the recent methods for the diagnosis and identification of NTM infections in clinical practice. In the future, the molecular-based diagnosis will significantly reduce the turnaround time of the diagnosis and thereby improving patient outcome.


International Journal of Infectious Diseases | 2011

PP-212 Rapid detection of Mycobacterium tuberculosis complex in spinal aspirate from cases of Pott's spine disease

S.G. Babu; Manoj Kumar; Rajesh Kumar; Vijaya Lakshmi Nag; U. Singh; Tapan N. Dhole

PP-212 Rapid detection of Mycobacterium tuberculosis complex in spinal aspirate from cases of Pott’s spine disease S.G. Babu1 *, M. Kumar1, R. Kumar2, V.L. Nag3, U. Singh4, T.N. Dhole3. 1Babasaheb Bhimrao Ambedkar University, Lucknow, India, 2Department of Neurosurgery, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India, 3Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India, 4Department of Biostatistics, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India


BioScience Trends | 2010

Multi-drug resistant tuberculosis: an iatrogenic problem.

Surya Kant; Anand Kumar Maurya; Rashmi Kushwaha; Vijaya Lakshmi Nag; Rajendra Prasad


Journal of Infection in Developing Countries | 2010

Assessment of World Health Organization definition of dengue hemorrhagic fever in North India

Prashant Gupta; Vineeta Khare; Sanjeev Tripathi; Vijaya Lakshmi Nag; Mohammad Yahiya Khan; Tapan N. Dhole

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Tapan N. Dhole

Sanjay Gandhi Post Graduate Institute of Medical Sciences

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Anand Kumar Maurya

King George's Medical University

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Manoj Kumar

Jaypee Institute of Information Technology

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Surya Kant

King George's Medical University

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Ram Awadh Singh Kushwaha

King George's Medical University

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Narendra Krishnani

Sanjay Gandhi Post Graduate Institute of Medical Sciences

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Raj Kumar

Sanjay Gandhi Post Graduate Institute of Medical Sciences

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Rajesh Kumar

Post Graduate Institute of Medical Education and Research

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Ram Nawal Rao

Sanjay Gandhi Post Graduate Institute of Medical Sciences

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