Vilnei Mattioli Leite
Federal University of São Paulo
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Vilnei Mattioli Leite.
Journal of Neuroscience Methods | 2008
Marcela Fernandes; Sandra Gomes Valente; Maria José da Silva Fernandes; Evandro Penteado Villar Félix; Maria da Graça Naffah Mazzacoratti; Débora Amado Scerni; João Baptista Gomes dos Santos; Vilnei Mattioli Leite; Flávio Faloppa
The aim of this study was to compare the outcomes of nerve autografts (GRF) and venous grafts containing mononuclear bone marrow cells (BMCs) in sciatic nerve-lesioned rats. Control animals underwent sham operations (SHAM), received empty venous grafts (EPV), or received venous grafts containing BMC vehicle (AGR). Outcome was evaluated through sciatic functional index (SFI), morphometric and morphologic analyses of the nerve distal to the lesion, and the number of spinal cord motor neurons positive for the retrograde tracer, Fluoro-Gold. All groups exhibited poor results in SFI when compared to SHAM animals throughout the postoperative period. All groups also had a significantly greater fiber density, decreased fiber diameter, and decreased motor neuron number than the SHAM group. No significant difference between the GRF and BMC groups was observed in any of these parameters. On the other hand, vessel density was significantly higher in BMC than all other groups. BMC-containing venous grafts are superior to nerve autografts in increasing vessel density during sciatic nerve regeneration.
Acta Ortopedica Brasileira | 2006
Lia Miyamoto Meirelles; João Baptista Gomes dos Santos; Luciana Leonel dos Santos; Marco Aurélio Branco; Flávio Faloppa; Vilnei Mattioli Leite; Carlos Henrique Fernandes
Entre os anos de 1995 e 1998, foram realizadas 112 cirurgias para tratamento da Sindrome do Tunel do Carpo (STC) pela tecnica de incisao palmar e utilizacao do retinaculo de Paine. Com o objetivo de avaliar os resultados em longo prazo, os pacientes foram convocados. Houve o retorno de 44 pacientes. Deste total, tres pacientes, por terem doencas associadas, foram excluidos, resultando, um total de 53 maos analisadas. Apresentaremos os resultados da avaliacao subjetiva, obtidos atraves da aplicacao de um teste de auto-avaliacao chamado de questionario de Boston. Este questionario consiste em perguntas que avaliam a gravidade dos sintomas e o estado funcional no momento da aplicacao do mesmo. Atraves da aplicacao do referido questionario encontramos um escore de 1,41 ± 0,57 para gravidade dos sintomas e 1,59 ± 0,93 para o estado funcional. Como este questionario nao foi aplicado no pre-operatorio deste grupo de pacientes analisados, comparou-se a pontuacao obtida com as encontradas na literatura pertinente. Os resultados obtidos demonstraram que as pontuacoes pos-operatorias sao similares aquelas existentes na literatura, mesmo sendo referidas a tempos diferentes de seguimento pos-operatorios, concluindo que havendo uma melhora dos sintomas, o questionario de Boston e sensivel a esta mudanca clinica.
Acta Ortopedica Brasileira | 2003
Marco Aurélio Sertório Grecco; Vilnei Mattioli Leite; Walter Manna Albertoni; João Baptista Gomes dos Santos; Celso Kiyoshi Hirakawa; Flávio Faloppa; Sandra Gomes Valente
The authors studied, in rats, of nerve regeneration in nerve grafts comparing with control group using Fluoro-Gold® (FG) labeled motor neurons count in spinal cord. In control group both tibial nerves were exposed to FG® and motor neurons counted in spinal cord in 48 hours, after perfusion, in a medular segment from L3 to S1. In experimental group, ressecting 8 mm, a gap was created in both tibial nerves and nerve segment of one nerve was used to repair contralateral side in a traditional nerve graft suture. After four months, right tibial nerve was exposed to FG® distal to graft and left tibial nerve exposed to FG proximal to graft. In 48 hours, after perfusion, motoneurons were counted in a medular segment from L3 to S1. In both groups medular segment was cut in 40 mm slices and all labeled cells counted. Wilcoxon and Student tests were used for statistical analysis. Control group presented a significative increased number of motoneurons when compared to experimental group. In experimental group number of motoneurons was significantly decreased when tibial nerve was exposed to FG® distal to nerve graft. Nerve graft promoted a partial block to axon migration after four months of surgery.
Journal of Reconstructive Microsurgery | 2014
Rodrigo Guerra Sabongi; Luiz Augusto Lucas Martins De Rizzo; Marcela Fernandes; Sandra Gomes Valente; João Baptista Gomes dos Santos; Flávio Faloppa; Vilnei Mattioli Leite
BACKGROUND In nerve injury with nervous gap, no restitution method was found better than the autograft, however, it has the disadvantage of damaging a normal nerve to be used as a graft. Platelet-rich plasma (PRP) is a possible filler material for vein grafts used as conduits for nerve regeneration, preventing its collapse, and providing growth factors and osteoconductive proteins. METHODS Isogenic rats were randomly divided into three groups. They received nerve autografts (GRF), PRP-containing vein grafts or a sham operation. Outcomes were evaluated by the sciatic functional index (SFI), morphometric, and morphologic analyses of the nerve distal to the lesion, and the number of spinal cord motoneurons positive for retrograde Fluoro-Gold (Santa Cruz Biotechnology, Inc., Dallas, TX) tracer. RESULTS The PRP and GRF groups had lower SFI values than the control animals throughout the postoperative period. The SFI was significantly higher in the PRP group than the GRF group at 90 days postoperatively (p = 0.011). Fiber diameter and number of motoneurons were significantly decreased in both the PRP and GRF groups, as compared with the control. CONCLUSION PRP within a vein conduit may be an effective alternative or adjuvant to GRF, the current preferred treatment for nerve injury with a nerve gap, and further investigations are required to fully define the role of PRP in nerve regeneration.
Acta Ortopedica Brasileira | 2012
Ewerton Bastos dos Santos; Marcela Fernandes; João Baptista Gomes dos Santos; Vilnei Mattioli Leite; Sandra Gomes Valente; Flávio Faloppa
Objective This study compared nerve regeneration in Wistar rats, using epineural neurorrhaphy with a gap of 1.0 mm and without a gap, both wrapped with jugular vein tubes. Motor neurons in the spinal cord between L3 and S1 were used for the count, marked by exposure of the tibial nerve to Fluoro-Gold (FG). Method The tibial nerves on both sides were cut and sutured, with a gap on one side and no gap in the other. The sutures were wrapped with a jugular vein. Four months after surgery the tibial nerves were exposed to Fluoro-Gold and the motor neuron count performed in the spinal cord. Results The results were statistically analyzed by the paired Wilcoxon test. There was a statistical difference between the groups with and without gap in relation to the motor neuron count (p=0.013). Conclusion The epineural neurorraphy without gap wrapped with jugular vein showed better results for nerve regeneration than the same procedure with gap. Level of Evidence: Experimental Study.OBJECTIVE: This study compared nerve regeneration in Wistar rats, using epineural neurorrhaphy with a gap of 1.0 mm and without a gap, both wrapped with jugular vein tubes. Motor neurons in the spinal cord between L3 and S1 were used for the count, marked by exposure of the tibial nerve to Fluoro-Gold (FG). METHOD: The tibial nerves on both sides were cut and sutured, with a gap on one side and no gap in the other. The sutures were wrapped with a jugular vein. Four months after surgery the tibial nerves were exposed to Fluoro-Gold and the motor neuron count performed in the spinal cord. RESULTS: The results were statistically analyzed by the paired Wilcoxon test. There was a statistical difference between the groups with and without gap in relation to the motor neuron count (p=0.013). CONCLUSION: The epineural neurorraphy without gap wrapped with jugular vein showed better results for nerve regeneration than the same procedure with gap. Level of Evidence: Experimental Study.
Revista Brasileira De Ortopedia | 2012
Mário Sérgio Viana Xavier; Vilnei Mattioli Leite
OBJECTIVE: To evaluate the effect of butyl-2-cyanoacrylate tissue adhesive in osteotomies and bone grafts, with regard to macroscopic and radiographic characteristics. METHODS: Forty-eight rabbits were used, randomly divided into four groups of 12 animals, with observation periods of two, four, eight and 16 weeks. Both thoracic limbs were operated in each animal and two osteotomies were performed in each of the radii, withdrawing a bone fragment (bone graft) of 1 cm in length. On one side, the bone graft was then replaced and a drop of adhesive was applied to each of the osteotomies. On the other side, the same procedure was performed without applying the adhesive. The rejection level for the nullity hypothesis was set at 0.05% or 5%. RESULTS: Blue marks were present in all the surgical specimens in which adhesive was applied. From the fourth week onwards, there was absence of movement of the bone grafts with adhesive and control. In group A, in the proximal osteotomies with adhesive, there was less deviation of the bone graft (p = 0.02). In group C, the union (p = 0.03) and the integration of the bone graft (p = 0.02) were better in the proximal osteotomies with adhesive. CONCLUSIONS: The adhesive was not completely metabolized within 16 weeks. There was clinical consolidation of the osteotomies within four weeks. The adhesive stabilized the bone graft within the first weeks and did not interfere with the consolidation of the osteotomies, or the integration of the bone graft in radiographic observations.
Revista Brasileira De Ortopedia | 2012
Mário Sérgio Viana Xavier; Vilnei Mattioli Leite
Objective: To evaluate the effect of butyl-2-cyanoacrylate tissue adhesive in osteotomies and bone grafts, with regard to macroscopic and radiographic characteristics. Methods: Forty-eight rabbits were used, randomly divided into four groups of 12 animals, with observation periods of two, four, eight and 16 weeks. Both thoracic limbs were operated in each animal and two osteotomies were performed in each of the radii, withdrawing a bone fragment (bone graft) of 1 cm in length. On one side, the bone graft was then replaced and a drop of adhesive was applied to each of the osteotomies. On the other side, the same procedure was performed without applying the adhesive. The rejection level for the nullity hypothesis was set at 0.05% or 5%. Results: Blue marks were present in all the surgical specimens in which adhesive was applied. From the fourth week onwards, there was absence of movement of the bone grafts with adhesive and control. In group A, in the proximal osteotomies with adhesive, there was less deviation of the bone graft (p = 0.02). In group C, the union (p = 0.03) and the integration of the bone graft (p = 0.02) were better in the proximal osteotomies with adhesive. Conclusions: The adhesive was not completely metabolized within 16 weeks. There was clinical consolidation of the osteotomies within four weeks. The adhesive stabilized the bone graft within the first weeks and did not interfere with the consolidation of the osteotomies, or the integration of the bone graft in radiographic observations.
Lasers in Medical Science | 2012
Carla Christina Medalha; Giuliana Castro Di Gangi; Caroline Bublitz Barbosa; Marcela Fernandes; Odair Aguiar; Flávio Faloppa; Vilnei Mattioli Leite; Ana Claudia Muniz Renno
Revista Acta Fisiátrica | 2003
Júnia Amorim Andrade; Vilnei Mattioli Leite; Luci Fuscaldi Teixeira-Salmela; Pola Maria Poli de Araújo; Yara Juliano
Revista Brasileira De Ortopedia | 2004
Edson S. Sato; Walter Manna Albertoni; Vilnei Mattioli Leite; João Baptista Gomes dos Santos; Flávio Faloppa