Vincent Guérin

Light and nitrogen nutrition regulate apical control in Rosa hybrida L.

Apical control is defined as the inhibition of basal axillary bud outgrowth by an upper actively growing axillary axis, whose regulation is poorly understood yet differs markedly from the better-known apical dominance. We studied the regulation of apical control by environmental factors in decapitated Rosa hybrida in order to remove the apical hormonal influence and nutrient sink. In this plant model, all the buds along the main axis have a similar morphology and are able to burst in vitro. We concentrated on the involvement of light intensity and nitrate nutrition on bud break and axillary bud elongation in the primary axis pruned above the fifth leaf of each rose bush. We observed that apical control took place in low light (92 μmol m(-2)s(-1)), where only the 2-apical buds grew out, both in low (0.25 mM) and high (12.25 mM) nitrate. In contrast, in high light (453 μmol m(-2)s(-1)), the apical control only operates in low nitrate while all the buds along the stem grew out when the plant was supplied with a high level of nitrate. We found a decreasing photosynthetic activity from the top to the base of the plant concomitant with a light gradient along the stem. The quantity of sucrose, fructose, glucose and starch are higher in high light conditions in leaves and stem. The expression of the sucrose transporter RhSUC2 was higher in internodes and buds in this lighting condition, suggesting an increased capacity for sucrose transport. We propose that light intensity and nitrogen availability both contribute to the establishment of apical control.

Low-amplitude, high-frequency electromagnetic field exposure causes delayed and reduced growth in Rosa hybrida

It is now accepted that plants perceive high-frequency electromagnetic field (HF-EMF). We wondered if the HF-EMF signal is integrated further in planta as a chain of reactions leading to a modification of plant growth. We exposed whole small ligneous plants (rose bush) whose growth could be studied for several weeks. We performed exposures at two different development stages (rooted cuttings bearing an axillary bud and 5-leaf stage plants), using two high frequency (900MHz) field amplitudes (5 and 200Vm(-1)). We achieved a tight control on the experimental conditions using a state-of-the-art stimulation device (Mode Stirred Reverberation Chamber) and specialized culture-chambers. After the exposure, we followed the shoot growth for over a one-month period. We observed no growth modification whatsoever exposure was performed on the 5-leaf stage plants. When the exposure was performed on the rooted cuttings, no growth modification was observed on Axis I (produced from the elongation of the axillary bud). Likewise, no significant modification was noted on Axis II produced at the base of Axis I, that came from pre-formed secondary axillary buds. In contrast, Axis II produced at the top of Axis I, that came from post-formed secondary buds consistently displayed a delayed and significant reduced growth (45%). The measurements of plant energy uptake from HF-EMF in this exposure condition (SAR of 7.2 10(-4)Wkg(-1)) indicated that this biological response is likely not due to thermal effect. These results suggest that exposure to electromagnetic field only affected development of post-formed organs.

Asparagine and sugars are both required to sustain secondary axis elongation after bud outgrowth in Rosa hybrida

Nitrogen is required for optimal plant growth, especially in young organs such as secondary axes (axes II) after axillary bud outgrowth. Several studies have shown an increase of nitrogen concentration in xylem sap concomitantly with bud outgrowth, but the relation between nitrogen, sugars and plant hormones in axis II still remains unclear. We investigated in Rosa hybrida the involvement of nitrogen nutrition in axis II elongation in relation with sugars and cytokinins using 15N-labeled nitrate and sugars, amino acids and cytokinin quantifications. Besides, we measured the effect of the exogenous supply of these compounds on axis II elongation using in vitro excised bud culture. We demonstrated that nitrogen in the axis II comes mainly from new root uptake after decapitation. Asparagine, which concentration increases in sap exudates and tissues during axis II elongation, was the sole amino acid able to sustain an efficient elongation in vitro when supplied in combination with sucrose.

Impacts of contrasting light on bud burst and on RwMAX1 and RwMAX2 expression in rose

Bud burst is a crucial factor in plant architecture and is strongly induced by light. In Rosa sp., this light effect was correlated with the growth of axillary buds and RwMAX1 and RwMAX2 expression within buds. In this paper, we investigated whether strigolactone pathway is involved in the regulation of axillary bud in response to light intensity. Hence, young roses were subjected to two contrasting light intensity regimes: high/high and high/low. The phenotype was characterized in both conditions and the expression of RwMAX1 and RwMAX2 genes was measured in the basal, middle and apical parts of rose primary branch. Light treatments showed a strong impact on axillary bud. The percentage of bud burst was severely reduced in the treatment high/low compared to the treatment high/high in all branch parts. In addition, the expression of RwMAX1 and RwMAX2 was strongly inhibited by high/high light regime and was conversely correlated with the rate of bud burst. In in vitro-grown axillary buds supplied with sucrose, glucose and fructose, RwMAX1 expression was significantly stimulated whereas that of RwMAX2 was significantly inhibited. Our results suggest that although RwMAX1 and RwMAX2 expression can be regulated by light, this expression does not explain the ability of bud burst.

Effect of fertigation timetable on growth and nitrogen content of an ornamental shrub (Ligustrum ovalifolium L.)

In order to limit nitrate leaching without affecting growth of soilless culture, a better adjustment between fertilisation timetable and nitrogen (N) seasonal demand of plants is required. One-year-old Ligustrum ovalifolium L. were grown outdoors in containers for two years. The first year plants received three different fertigation -water and nutrients- timings (continuous, during spring and autumn or only during spring). The second year, only water was provided, compelling plants to use their reserves for regrowth. Growth and total N were determined for nineteen destructive harvests along the two years. Stopping fertigation in summer had no immediate effect on growth. Restoring fertigation in autumn (did not) increase current growth but allowed N pool reconstitution. Plants receiving continuous fertigation and spring and autumn fertigation had an identical regrowth. Regrowth was linked to an identical N status of both plants in winter. Treatment combining spring and autumn fertigation allowed to limit nitrate supply and to ensure a sufficient N storage to support regrowth.

Absorption, storage and mobilisation of nitrogen and phosphorus in an ornamental shrub (Ligustrum ovalifolium) during a two year fertilisation experiment.

For soilless ligneous plant culture, improving the fertilisation schedule requires better knowledge of storage dynamics and nutrient remobilisation. Ligustrum ovalifolium was grown for fifteen months with two different fertilisation treatments during the first year [spring, summer and autumn (SsA) or spring and autumn (SA) and no fertilisation the second year. Growth, nitrogen (N) and phosphorus (P) contents were measured throughout the experiment (18 sampling dates) in roots, old and young leaves, old and young stems. Stopping fertilisation during the summer of the first year affected neither the next spring plant regrowth nor the N and P remobilisation towards young ramifications. The evolution of N and P contents in the whole plant was strongly linked. Nitrogen was preferentially stored in and mobilised from the perennial aerial parts of the plant whereas phosphorus was stored in and mobilised from all the perennial parts of the shrub.