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Dive into the research topics where Vincenzo Cupelli is active.

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Featured researches published by Vincenzo Cupelli.


Analytica Chimica Acta | 2011

Fully automated solid-phase microextraction-fast gas chromatography-mass spectrometry method using a new ionic liquid column for high-throughput analysis of sarcosine and N-ethylglycine in human urine and urinary sediments.

Stefano Dugheri; M. Musci; A. Bonacchi; E. Salvadori; Giulio Arcangeli; Vincenzo Cupelli; Michele Lanciotti; L. Masieri; Sergio Serni; Marco Carini; Maria Careri; A. Mangia

A fully automated, non invasive, rapid and high-throughput method for the direct determination of sarcosine and N-ethylglycine in urine and urinary sediments using hexyl chloroformate derivatization followed by direct immersion solid-phase micro extraction and fast gas chromatography-mass spectrometric analysis was developed and validated. The use of a new ionic liquid narrow bore column, as well as the automation and miniaturization of the preparation procedure by a customized configuration of the utilized XYZ robotic system, allowed a friendly use of the GC apparatus achieving a quantitation limit of 0.06 μg L(-1) for sarcosine, good repeatability with CV always lower than 7% and reduced analysis times useful for point-of-care testing. The method was then applied for the analysis of 56 samples of urine and urinary sediments in healthy subjects, in those with benign prostatic hypertrophy and in patients with clinically localized prostate cancer. The results obtained showed that the medians of sarcosine/creatinine in urine were 103, 137 and 267 μg g(-1) respectively, thus assessing the potential use of sarcosine as urinary biomarker for prostate cancer detection. The highest values of sensitivity (79%) and specificity (87%) were obtained in correspondence of a cut-off value of 179 μg sarcosine(g creatinine)(-1), thus by using this cut-off threshold, sarcosine was significantly associated with the presence of cancer (p<0.0001). Finally, ROC analyses proved that the discrimination between clinically localized prostate cancer and patients without evidence of tumor is significantly correlated with sarcosine.


The Cardiology | 1989

Relationship between age and anterograde refractoriness of the accessory pathway in Wolff-Parkinson-White patients.

Antonio Michelucci; Luigi Padeletti; Alessandro Mezzani; Tiziana Giovannini; Michele Miceli; Vincenzo Cupelli; Roberto Musante

In 22 patients (age range 13-40 years) with Wolff-Parkinson-White ECG pattern without evidence of associated cardiomyopathy we measured the anterograde effective refractory period of the accessory pathway (ERP-AP) by extrastimulus method (at twice diastolic threshold) during atrial pacing (100/min). The ERP-AP range was 220-480 ms. There was a significant direct correlation between age and ERP-AP (r = 0.50, p less than 0.01). An ERP-AP less than or equal to 250 ms was found in 4 patients (age less than or equal to 23 years). This is noteworthy in the light of reports that, over the years: (1) typical Wolff-Parkinson-White ECG signs can disappear and (2) the frequency of tachycardic episodes decreases. Our data suggest a lower risk of high ventricular rates during atrial fibrillation with increasing age.


Biomedical Chromatography | 2008

Determination of organic acids in urine by solid‐phase microextraction and gas chromatography–ion trap tandem mass spectrometry previous ‘in sample’ derivatization with trimethyloxonium tetrafluoroborate

Marco Pacenti; Stefano Dugheri; Fabio Villanelli; Gianluca Bartolucci; Luca Calamai; Pierpaolo Boccalon; Giulio Arcangeli; Febe Vecchione; Paolo Alessi; Ireneo Kikic; Vincenzo Cupelli

A method for the determination of the organic acids directly in the urine employing derivatization with trimethyloxonium tetrafluoroborate as a methylating agent and sequential extraction by head space and direct immersion/solid phase microextraction is reported. Furoic acid, hippuric acid, methylhippuric acid, mandelic acid, phenylglyoxylic acid and trans, trans muconic acid contained in urine and proposed by the American Conference of Governmental Industrial Hygienists as biological exposure indices were determined after a fast and economically convenient preparation step and sensitive gas chromatography-ion trap-mass spectrometry/tandem mass spectrometry analysis. Urine is rather a complex sample and hence the acquisition method required specific GC-MS instrumentation capable of supporting the changeover, fully automated during a single chromatographic separation, from mass to tandem mass spectrometry and both chemical and electron ionization modes. The automation of the analytical method provides a number of advantages, including reduced analysis time for both routine analysis and method development, and greater reproducibility. The equilibrium and kinetics of this substances vs head space/direct immersion-solid phase microextraction were investigated and evaluated theoretically.


Science of The Total Environment | 2001

Effects of silica on human lung fibroblast in culture

Giulio Arcangeli; Vincenzo Cupelli; G. Giuliano

Silica has been reported to directly stimulate cellular proliferation of human lung fibroblasts, and silica-treated macrophage supernatants induce fibroblast proliferation and some of their biosynthetic activities. Alveolar macrophages produce increased amount of tumour necrosis factor alpha (TNF-alpha) and transforming growth factor beta (TGF-beta). Lung fibroblasts are producers of interleukin-6 (IL-6). We investigated the capacity of lung fibroblasts obtained from normal and silicosis subjects to elaborate IL-6 in response to TNF-alpha and to TGF-beta. Our data show that TNF-alpha and TGF-beta are able to stimulate the proliferation of human lung fibroblasts in culture, to increase the collagen production of the cells and are both able to increase IL-6 production by lung fibroblasts of patients with silicosis. We hypothesise that silica is able to stimulate lung fibroblast both directly, increasing the cell proliferation, and indirectly stimulating the release of factors (as TNF-alpha and TGF-beta) from activated alveolar macrophages, that are able to increase proliferative and biosynthetic activities of fibroblast.


Industrial Health | 2014

Do I Just Look Stressed or am I Stressed? Work-related Stress in a Sample of Italian Employees

Gabriele Giorgi; Jose M. Leon-Perez; Vincenzo Cupelli; Nicola Mucci; Giulio Arcangeli

Work-related stress is becoming a significant problem in Italy and it is therefore essential to advance the theory and methodology required to detect this phenomenon at work. Thus, the aim of this paper is to propose a new method for evaluating stress at work by measuring the discrepancies between employees’ perceptions of stress and their leaders’ evaluation of the stress of their subordinates. In addition, a positive impression scale was added to determine whether workers might give socially desirable responses in organizational diagnosis. Over 1,100 employees and 200 leaders within several Italian organizations were involved in this study. Structural equation modeling was used to test such new method for evaluating stress in a model of stress at work that incorporates relationships among individual (positive impression), interpersonal (workplace bullying) and organizational factors (working conditions, welfare culture, training). Results showed that the leaders’ capacity to understand subordinates’ stress is associated with subordinates’ psychological well-being since higher disagreement between self and leaders’ ratings was related to lower well-being. We discuss the implications of healthy leadership for the development of healthy organizations.


Journal of Chromatography A | 2014

Ionic liquid-based solid phase microextraction necklaces for the environmental monitoring of ketamine

Franco Bisceglie; Stefano Dugheri; Giulio Arcangeli; Vincenzo Cupelli; Elia Del Borrello; Len Sidisky; Maria Careri

Wearable solid phase microextraction (SPME) devices consisting in necklaces and pins were developed for the environmental monitoring of ketamine in recreational places using ionic liquid as coating. SPME fibers obtained using both monocationic and dicationic polymeric ionic liquids were characterized in terms of morphology, film thickness, thermal stability and pH resistance. An average thickness of 30±5μm, an excellent thermal stability until 350°C and a very good fiber-to-fiber and batch-to-batch repeatability with RSD lower than 4% were some of the features of the developed coatings. A quantitation limit (LOQ) of 0.05mg/m(3) with a sampling time of 1min proved the feasibility of the developed method for the quantitation of ketamine in air at low concentration levels. Finally, the capabilities of the fibers for the rapid SPME sampling of ketamine in recreational places were proved obtaining extraction efficiencies at least two-fold higher than those obtained using commercial devices and extraction recoveries ranging from 84.2±3.3% to 93.6±2.6% (n=3).


Science of The Total Environment | 2015

Work-related stress assessment in a population of Italian workers. The Stress Questionnaire

Nicola Mucci; Gabriele Giorgi; Vincenzo Cupelli; Pier Agostino Gioffrè; Maria Valeria Rosati; Francesco Tomei; Gianfranco Tomei; Edgar Breso-Esteve; Giulio Arcangeli

The present study shows detailed information about the reliability and validity of the psychosocial risk scale included in the Stress Questionnaire (SQ) developed by our research group. The primary purpose of this work is to test the factor structure of the psychosocial risk scale through a first-order confirmatory factor analysis (CFA) using a large pooled dataset obtained from a population of 2026 employees of 15 Italian medium-large companies. Data were collected by a team of researchers who examined demographic variables, work-related stress, workplace bullying, mental health and other constructs. In addition to these substantive issues, the survey was designed to better understand response bias. After the evaluation of the results we conclude that the psychosocial risk scale reported a satisfactory reliability and validity. In addition, it allowed a careful measurement of work related stress, considering both leaders and followers perspectives.


The Cardiology | 1990

Effects of Training on the Electrophysiologic Properties of Atrium and Accessory Pathway in Athletes with Wolff-Parkinson-White Syndrome

Alessandro Mezzani; Tiziana Giovannini; Antonio Michelucci; Luigi Padeletti; Angelo Resina; Vincenzo Cupelli; Roberto Musante

Twenty-two subjects with Wolff-Parkinson-White (WPW) electrocardiographic pattern performing agonistic physical activity were referred to our laboratory to assess arrhythmogenic risk (group 1). This allowed us to evaluate a less known aspect, namely that of effects of training on the electrophysiologic properties of the atrium and accessory pathway. This was done utilizing a control group of 10 WPW patients who did not perform agonistic physical activity (group 2). All subjects were symptom free, and without signs of associated cardiopathy if we exclude 1 patient of group 1, who presented moderate mitral valve prolapse. Group 1 patients showed significantly higher mean values for basic cycle length (p less than 0.001), atrial effective (p less than 0.04) and functional (p less than 0.02) refractory period, and anterograde effective refractory period of the accessory pathway (p less than 0.02). The different behavior observed in group 1 patients could be explained considering the known influence of training on the equilibrium of the autonomic nervous system. Moreover, it is noteworthy that the two groups did not differ for inducibility of atrial fibrillation (AF). This should be taken into account considering the importance of AF in WPW. In conclusion, our study does not demonstrate any negative electrophysiologic effects of training in patients with WPW.


Chemosphere | 2016

Workers exposed to low levels of benzene present in urban air: Assessment of peripheral blood count variations

T. Casale; C. Sacco; Serafino Ricci; Beatrice Loreti; Alessandro Pacchiarotti; Vincenzo Cupelli; Giulio Arcangeli; Nicola Mucci; Vittorio Antuono; Federica De Marco; Gianfranco Tomei; Francesco Tomei; Maria Valeria Rosati

BACKGROUND Few studies in the literature have examined the effects of benzene on blood cells. AIM The aim of this study was to evaluate the possible correlation between the blood benzene levels and the blood cell counts. MATERIALS AND METHODS From a population of 2658 workers, we studied a group of 215 subjects. Each worker underwent blood sampling for the assessment of the blood benzene levels and the blood cell counts. The Mann-Whitney U test for two-mode variables and the Kruskal-Wallis test for more-than-two-mode variables were performed on all subjects. We estimated the Pearson correlation index between the variables in the total sample and the subgroups divided according to sex, the smoking habit, and job. After the main confounding factors were evaluated, multiple linear regression was performed on both the total sample and the subgroups. RESULTS A significant inverse correlation was found among the blood benzene levels and the white blood cells, lymphocytes, and neutrophils in traffic policemen, motorcyclists, and other outdoor workers. We did not find any significant correlation with any other parameters of blood cell count. DISCUSSION AND CONCLUSIONS Our results, which must be considered preliminary, indicate that increased blood benzene levels in outdoor workers lead to decreased counts of white blood cells, neutrophils, and lymphocytes, because of possible immune effects. These are worth investigating in the future by specific immune tests.


Psychology Research and Behavior Management | 2015

Psychometric properties of the impact of event scale-6 in a sample of victims of bank robbery

Gabriele Giorgi; Francisco Saverio Fiz Perez; Andrea Castiello D’Antonio; Nicola Mucci; Claudia Ferrero; Vincenzo Cupelli; Giulio Arcangeli

Bank robberies are becoming a serious problem in Italy. This study aims to evaluate the validity and the reliability of the Italian version of Impact of Event Scale (IES)-6. It is also hypothesized that a potential posttraumatic disorder, as measured by the IES-6, is associated with mental health problems and several peritraumatic variables. A database was built from data collected through a survey of victims of robbery in an Italian bank. The final sample comprised 350 employees. This study tests different models of IES, comparing the validity of IES-6 with the 22-item original version (IES-R) and the 15-item Italian version (recently adopted in a sample of flood victims). A confirmatory factor analysis supported the IES-6 three-factor solution as the best model. In addition, the internal consistency of the IES-6 and the subscales was good. Outcomes revealed a robust structure supporting the composition of the IES-6 Italian version.

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Gabriele Giorgi

Sapienza University of Rome

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