Vincenzo Maccarrone

Behavioural and biochemical stress responses of Palinurus elephas after exposure to boat noise pollution in tank.

This study examined the effects of boat noise on the behavioural and biochemical parameters of the Mediterranean spiny lobster (Palinurus elephas). The experiment was conducted in a tank equipped with a video and audio recording system. 18 experimental trials, assigned to boat noise and control conditions, were performed using lobsters in single and group of 4 specimens. After a 1h habituation period, we audio- and video-recorded the lobsters for 1h. During the experimental phase, the animals assigned to the boat groups were exposed to boat noise pollution (a random sequence of boat noises). Exposure to the noise produced significant variations in locomotor behaviours and haemolymphatic parameters. Our results indicate that the lobsters exposed to boat noises increased significantly their locomotor activities and haemolymphatic bioindicator of stressful conditions such as glucose, total proteins, Hsp70 expression and THC when tested both singly and in groups.

The underwater acoustic activities of the red swamp crayfish Procambarus clarkii

This study describes the underwater acoustic behavior of the red swamp crayfish Procambarus clarkii. The study was conducted both in a tank and in the natural environment. The tank was equipped with video and acoustic recording systems. Observations were conducted to identify the underwater acoustic signals produced and their association with behavioral events and the movement status of the animals. In a lake in a natural reserve, a remote acoustic recording station was used to study the circadian underwater acoustic activity of the crayfish and to assess the acoustic features of the signals. The red swamp crayfish produces irregular trains of wide-band pulses (duration 0.4 ms, SPL(PK) 128 dB re 1 μPa, peak frequency 28 kHz, bandwidth(RMS) 20 kHz). The production of signals is positively related to intraspecific interactions (encounter/approach, fighting and successive Tail Flips). In the natural environment, acoustic activity is almost absent during the day, increases abruptly at sunset and continues until dawn. This study reveals the previously unknown underwater acoustic signals of Procambarus clarkii and the potential of passive acoustic methods to monitor the presence, the abundance and the behavioral activities of this invasive species.

Temporal patterns in the soundscape of the shallow waters of a Mediterranean marine protected area.

The study of marine soundscapes is an emerging field of research that contributes important information about biological compositions and environmental conditions. The seasonal and circadian soundscape trends of a marine protected area (MPA) in the Mediterranean Sea have been studied for one year using an autonomous acoustic recorder. Frequencies less than 1 kHz are dominated by noise generated by waves and are louder during the winter; conversely, higher frequencies (4–96 kHz) are dominated by snapping shrimp, which increase their acoustic activity at night during the summer. Fish choruses, below 2 kHz, characterize the soundscape at sunset during the summer. Because there are 13 vessel passages per hour on average, causing acoustic interference with fish choruses 46% of the time, this MPA cannot be considered to be protected from noise. On the basis of the high seasonal variability of the soundscape components, this study proposes a one-year acoustic monitoring protocol using the soundscape methodology approach and discusses the concept of MPA size.

Behavioural responses of the European spiny lobster, Palinurus elephas(Fabricius, 1787), to conspecific and synthetic sounds

This study examined the effects of three different acoustic stimuli on the behaviour of the European spiny lobster, Palinurus elephas(Fabricius, 1787). Twenty male and 20 female lobsters were exposed to: a low-frequency linear sweep tone (0.1-20 kHz), a high-frequency linear sweep tone (20-80 kHz), conspecific sounds (rasp: train of wideband pulses), and no sound stimulation (control). After a habituation period, lobsters were audio-video recorded for 30 min. Tail flipping, sound emissions, distance moved, mobility, velocity and movement were recorded. Differences between the males and females in the rasp playback condition were observed, with the females having highest values of the behaviours under study. Moreover, when analysing the male and female combined data we found that exposure to synthetic stimuli did not produce any significant variations. Finally, it can be assumed that there is: a capacity for rasp sound perception, and a significant role for these pulses in the intraspecific communication of P. elephas.

Depredation of striped dolphin on squid fishery and behavioural responses to interactive pinger.

The depredation of dolphins on some artisanal fisheries in the Mediterranean Sea is the major source of economic loss. This study aims to reveal the behavior of striped dolphin during interaction with flying squid fishery equipped with interactive pinger in the Ionian Sea (southern Italy). A four channel acoustics acquisition system was used during fishing hauls to record the clicks and to localize the positions of dolphins through the time delay of arrival method. The preliminary analysis shows that dolphins approached the artificial light, used for attract the squids, diving further on 100 m below the fishing boat. The number and the power of dolphins’ clicks decreased after the signals emitted by the interactive pinger. The distances of dolphins from fishing boat do not seem change significantly before and after pinger emissions. Although the efficiency of DDD pingers to decrease the depredation level was demonstrated in some study, the functioning mechanism is still unknown. Our data could indicate th...

Some consideration about the management and economic aspect on the pinger use for mitigation the depredation phenomena in an MPA Mediterranean area.

This study was carried out in a fishing area off the coast of southern Italy, where Tursiops truncatus is frequently observed to interact with bottom gill nets. The experiments aimed to assess the economic efficiency of the DDD pingers during the interaction between dolphins and monofilament bottom gill nets. A net was equipped with pingers and another without to measure the different biomass catch and costs. The economic effectiveness with an estimate of the costs/benefits was evaluated. We modeled a catch per unit effort (CPUE) both in economic terms as euros per 50 m of net set and in biomass terms as kilograms caught per 50 m of net set with and without pingers. The analysis of the data allowed us to evaluate the advantage of the economic aid provided by the European Fisheries Fund (EFF) for the use of pinger. The CPUE of the net equipped with pingers was statistically higher than the control net. The costs of investment for pingers could fully comeback in 74 days and with the economic assistance of EFF in 34 days of fishing. The use of pingers represents productive and economic benefits, even if their potential impact noise should be considered.