Vinita Tiwari

Shoot regeneration and somatic embryogenesis from different explants of Brahmi ( Bacopa monniera (L.) Wettst.)

Abstract The morphogenetic potential of node, internode and leaf explants of Brahmi [Bacopa monniera (L.) Wettst.] was investigated to develop reliable protocols for shoot regeneration and somatic embryogenesis. The explants were excised from shoots raised from axillary buds of nodal explants cultured on Murashige and Skoog (MS) basal medium. Presence of 6-benzylaminopurine (BA) or kinetin influenced the degree of callus formation, from which a large number of shoot buds regenerated. Leaf explants gave the largest number of shoot buds followed by node and internode explants. BA was superior to kinetin; BA at 1.5 – 2.0 mg/l appeared to be optimum for inducing the maximum number of shoot buds. MS + 0.1 mg/l BA + 0.2 mg/l indole-3-acetic acid was the most suitable for shoot elongation. Elongated shoots were rooted on full- or half-strength MS medium with or without 0.5 – 1.0 mg/l indole-3-butyric acid or 0.5 – 1.0 mg/l α-naphthaleneacetic acid. The rooted plants were successfully established in soil. Calli derived from nodal explants cultured on MS medium containing 0.5 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), when subcultured on MS medium containing 0.1 or 0.5 mg/l BA or 0.2 mg/l 2,4-D + 0.1 or 0.5 mg/l kinetin, developed somatic embryos. The somatic embryos germinated either on the same media or on MS basal medium, and the resulting plantlets were successfully transplanted to soil.

Awareness Regarding Risk Factors, Symptoms and Treatment Facilities for Cancer in Selected States of India

OBJECTIVE To study the level of awareness and knowledge about cancers and associated risk factors among households in selected states of India. METHODS In the study 3070 households were interviewed from six states viz, West Bengal, Kerala, Madhya Pradesh, Rajasthan and Mizoram. RESULTS Knowledge of cancers other than those related to tobacco was very low (prostate 8%, colon 11% ) among the communities, with a poor awareness of warning signs and symptoms. The knowledge varied from state to state. It is found that the major source of information related to cancers was television (38%) followed by friends and relatives (36%). Only about 15 % of respondents had knowledge about cancer awareness camps organized in their districts but they did not have knowledge about the organizers of the camp. Findings suggested a strong need for strengthening of DCCP. CONCLUSION It is important to create awareness among community through educational programs on cancer prevention, preventable cancer risk factors, benefits of early diagnosis, and availability of screening facilities. Integration of District Cancer Control activities with NRHM could be the most cost-effective strategy to prevent cancers and rural population.

Implementing Rashtriya Swasthya Bima Yojana: Experience from Delhi

Rashtriya Swasthya Bima Yojana aims to improve access to quality health care and relieve the burden of health care costs of the poor population. This article, based on interviews conducted with different stakeholders of the scheme during October–December 2010, looks at the implementation of the scheme in Delhi. A large proportion of the beneficiaries were unaware about the features and benefits of the scheme. A significant proportion of the families availing benefits had incurred the out-of-pocket expenses. The coverage of population was very low. The empanelled hospitals/nursing homes were hesitant in admitting patients with chronic ailments requiring continuous treatment at the hospital. Low package rates and delay in settlement of claims were reported as the major issues by the empanelled hospitals.