Vivek Krishna

Antioxidant study and assignments of NMR spectral data for 3', 4', 7-trihydroxyflavanone 3', 7-di-o-β-d-glucopyranoside (butrin) and its hydrolyzed product

The NMR spectral data including high resolution 1H, 13C and 2D NMR for butrin, 3′,4′,7-trihydroxyflavanone 3′,7-di-O-β-D-glucopyranoside, isolated from flowers of Butea monosperma, are reported here for the first time. Butrin was hydrolyzed using b-glucosidase to butin in high yield. They were subjected to free radical scavenging test using 2,2-diphenyl-1-picrylhydrazyl (DPPH) spectrophotometric assay. At a dose of 4 × 10-8 mol of tested compounds, the percentage of reduced DPPH for butin was 14.5% while no reduction was observed for butrin (0%).