Vladimíra Vallová

Initial Experience with Determination of hTERC and MYCC Amplification in Cervical Intraepithelial Neoplasia and Cervical Carcinoma in the Czech Republic

Tumors are frequently characterized by series of cytogenetic abnormalities. Amplifications of hTERC (3q26) and MYCC (8q24) genes have been associated with cervical intraepithelial neoplasia (CIN) and carcinoma of uterine cervix. The results of genetic analysis could select patients with high risk of progression from CIN to carcinoma. In this study, chromosomal abnormalities in cytology specimens of cervical carcinoma or CIN of 26 patients were analyzed using new developed triple-colour HPV-FISH assay. HPV infection was proven in 85 % (22/26) of patients. Amplification of hTERC and MYCC gene was found in 46 % (12/26) and 62 % (16/26) of patients, respectively. Based on these results we were able to divide patients into high-risk, medium-risk and low-risk group. We confirmed that HPV-FISH assay can be used as an effective diagnostic procedure to identify patients carrying highly risking HPV infection and chromosomal aberrations associated with this malignancy. Patients of the high-risk group would benefit from intensive dispensarisation and aggressive therapy in the future. Tumors are frequently characterized by series of cytogenetic abnormalities. Amplifications of hTERC (3q26) and MYCC (8q24) genes have been associated with cervical intraepithelial neoplasia (CIN) and carcinoma of uterine cervix. The results of genetic analysis could select patients with high risk of progression from CIN to carcinoma. In this study, chromosomal abnormalities in cytology specimens of cervical carcinoma or CIN of 26 patients were analyzed using new developed triple-colour HPV-FISH assay. HPV infection was proven in 85 % (22/26) of patients. Amplification of hTERC and MYCC gene was found in 46 % (12/26) and 62 % (16/26) of patients, respectively. Based on these results we were able to divide patients into high-risk, medium-risk and low-risk group. We confirmed that HPV-FISH assay can be used as an effective diagnostic procedure to identify patients carrying highly risking HPV infection and chromosomal aberrations associated with this malignancy. Patients of the high-risk group would benefit from intensive dispensarisation and aggressive therapy in the future.