Vlastimil Štětina

Properties of the strains Enterococcus haemoperoxidus and E.moraviensis, new species among enterococci

Antibiotic susceptibility or resistance, urease activity, detection of the structural genes for bacteriocin production, bacteriocin activity as well as sensitivity of the isolates to enterocins (Ent) A and M were determined in 23 isolates of new speciesEnterococcus haemoperoxidus andE. moraviensis. The majority of the strains were antibiotic sensitive and exhibited low urease activity (<10 nkat/mL). The most frequently detected genes for Ent wereentA andentP. However, only the strain 466 ofE. haemoperoxidus produced an antibacterial substance with inhibitory activity against 21 G+ indicators. It was partially purified reaching an activity of up to 12 800 AU/mL. This bacteriocin active strain also possessed the genes for EntA and EntP. The other strains did not inhibit the indicator strains. The substance produced by the 466 strain was active even after a 5-months storage at +4 and −20°C. This substance has proteolytic and hydrophilic character, pH optimum of bacteriocin production by this strain being between 4 and 7. WhileE. moraviensis strains showed sensitivity to EntA (produced byE. faecium EK13) and to EntM (produced byE. faecium AL41),E. haemoperoxidus strains were sensitive to EntA (except strain 382) but less sensitive to the treatment by EntM.

Identification of Staphylococcus piscifermentans from dog feces

The AD 2 strain isolated from feces of a healthy dog in Slovakia was characterized phenotypically by the conventional tests and commercial identification kits API Staph and ID32 Staph. Results of biochemical tests identified the strain asS. piscifermentans, fully corresponding with the species description. Further characterization by whole-cell protein profile analysis (SDS-PAGE) confirmed the identification based on biochemical tests and showed that the AD 2 strain isS. piscifermentans; lactic acid production, urease activity, bacteriocin production and the antibiotic susceptibility of it were also determined.S. piscifermentans AD 2 isolated first from an animal source was deposited in theCzech Collection of Microorganisms asStaphylococcus piscifermentans CCM 7165.