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Featured researches published by Volker Büttcher.


Biochimica et Biophysica Acta | 1999

MOLECULAR CLONING, FUNCTIONAL EXPRESSION AND PURIFICATION OF A GLUCAN BRANCHING ENZYME FROM NEISSERIA DENITRIFICANS

Volker Büttcher; Martin Quanz; Lothar Willmitzer

The nucleotide sequence containing the complete structural information for a glucan branching enzyme was isolated from a Neisseria denitrificans genomic library. The gene was expressed in Escherichia coli and the active recombinant protein was purified. The deduced protein of 762 amino acids with a calculated molecular weight of 86313 Da shows similarity to the primary protein sequences of other known glucan branching enzymes. Amino acid sequencing of the isolated protein by Edman degradation confirmed the deduced start codon of the structural gene of the glucan branching enzyme. The purified glucan branching enzyme has a stimulating effect on the Neisseria amylosucrase activity.


Archive | 1995

DNA SEQUENCES CODING FOR ENZYMES CAPABLE OF FACILITATING THE SYNTHESIS OF LINEAR α-1,4 GLUCANS IN PLANTS, FUNGI AND MICROORGANISMS

Jens Kossmann; Volker Büttcher; Thomas Welsh


Archive | 1999

Nucleic acid molecules which code a branching enzyme from bacteria of the genus neisseria, and a method for producing alpha-1,6-branched alpha-1,4-glucans

Volker Büttcher; Martin Quanz


Archive | 2007

Method for producing alpha-1, 6-branched alpha-1, 4-glucans from sucrose

Volker Büttcher; Martin Quanz


Archive | 1995

Microorganisms permitting the intracellular polyhydroxy alkanoate synthesis with simultaneous extracellular polysaccharide synthesis and processes for producing the same

Jens Kossman; Volker Büttcher; Thomas Welsh


Archive | 2000

Modified starch from plants, plants synthesizing the same, as well as process for their production

Jens Kossmann; Franziska Springer; Volker Büttcher


Archive | 1999

Method for producing α-1, 6-branched α-1, 4-glucans from sucrose

Volker Büttcher; Martin Quanz


Archive | 1999

Nucleinssäuremoleküle codierend ein Verzweigungsenzym aus Bakterien der Gattung Neisseria sowie Verfahren zur Herstellung von alpha-1,6-verzweigten alpha-1,4-Glucanen

Volker Büttcher; Martin Quanz


Archive | 1999

MOLECULAS DE ACIDOS NUCLEICOS QUE CODIFICAN UNA ENZIMA RAMIFICADORA PROCEDENTE DE BACTERIAS DEL GENERO NEISSERIA, ASI COMO PROCEDIMIENTOS PARA LA PREPARACION DE ALFA-1,4-GLUCANOS RAMNIFICADOS EN LAS POSICIONES ALFA-1,6.

Volker Büttcher; Martin Quanz


Archive | 1999

Nucleic acid molecules encodeing a branching enzyme from bacteria of the genus neisseria as well as methods for the production of .alpha.-1,6-branched .alpha.-1,4-glucans

Volker Büttcher; Martin Quanz

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