Volker Höllt

Endogenous opioid peptides: comparative evaluation of their receptor affinities in the mouse brain.

The affinities of certain endogenous opioid peptides and related sequences for mu, delta and k opiate receptors have been determined in membrane preparations from mouse brain. It was found that the KIs for the delta receptor changed very little when the sequence of the enkephalins was enlarged; on the contrary, the mu and especially the k activity were highly dependent not only on the specificity of the sequence but also on the length of the peptide. Most of the peptides have similar affinities for more than one receptor type. The enkephalins are the most selective for the delta receptor. beta-Endorphin, BAM-12P, BAM-22P, peptide E and dynorphin A display the best potency at the mu site. Dynorphin A (1-8), dynorphin A and dynorphin B are the most selective for the k site.

Acute δ-opioid receptor activation induces CREB phosphorylation in NG108-15 cells

Abstract A growing body of evidence supports an important role of the transcription factor cAMP responsive element binding protein (CREB) in mediating opioid-induced changes in the cAMP pathway. Regulation of CREB and subsequent changes in gene expression may underlie some long-term cellular adaptations associated with the administration of opioid drugs. The effect of morphine on the level of the transcription factor CREB, as well as CREB phosphorylation, was investigated in NG108-15 cells. Morphine and the δ-opioid receptor agonist [ d -Pen 2,5 ]enkephalin (DPDPE) produced a dose-dependent increase in CREB phosphorylation. The effect was reversed by naloxone and naltrindole, respectively. The calmodulin antagonist N -(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride (W-7), the protein kinase inhibitor staurosporine, as well as 1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride (H-7), an inhibitor of protein kinase C and cAMP-dependent protein kinase, but not N -[2-(methylamino)ethyl]-5-isoquinolinesulfonamide dihydrochloride (H-8), an inhibitor of cAMP- and cGMP-dependent protein kinase, blocked the opioid-induced CREB phosphorylation. The obtained results suggest that in the cells studied opioids affect, via the δ-opioid receptor, stimulatory intracellular mediator systems involving Ca 2+ /calmodulin and the protein kinase C pathway.

Pro-enkephalin intermediates in bovine brain and adrenal medulla: Characterization of immunoreactive peptides related to BAM-22P and peptide F

Using antibodies against the synthetic opioid peptides BAM-22P and peptide F, immunoreactive (ir-) peptides were measured in bovine brain and adrenal medulla. In addition to the high levels in the adrenal medulla, both ir- peptides were measurable in various areas of the brain with highest concentrations in the anterior hypothalamus. Analysis of the ir- components by gel filtration revealed molecular heterogeneity. Besides peptides with the size of BAM-22P or peptide F, various higher molecular weight species were found. These forms were found in the adrenal medulla in much higher concentrations than in the brain indicating a different processing mechanism for proenkephalin. Synthetic BAM-22P injected intracerebroventricularly into mice produces a substantial analgesia (ED50 6.4 nmole) which is almost as high as that of morphine (ED50 2.8 nmole). This finding and the presence of BAM-22P-like compounds in the brain suggests a role of the enkephalinergic system in pain transmission.

K receptor activities of the three opioid peptide families

A large number of opioid peptides derived from the three established precursors, pro-opiomelanocortin (POMC), pro-enkephalin A (pro-enk A), and pro-enkephalin B (pro-enk B) were tested for their ability to inhibit electrically induced contractions of the isolated rabbit vas deferens, a preparation sensitive to k but not to mu opioid ligands. In the presence of enzyme inhibitors, all peptides exhibit roughly similar potencies, but the shorter peptides display lower potencies in the absence of enzyme inhibitors. This suggests that the loss in activity is due to their enzymatic degradation. It is concluded that the pro-enk B gene codes peptides with high k-receptor activities, whereas peptides produced by the POMC gene are devoid of k-receptor activity and the peptides, coded by the pro-enk A gene exhibit weak to moderate k-receptor activity.

Extrahypothalamic Corticotropin and α-Melanotropin in Human Brain

The distribution of corticotropin (ACTH) and α -melanotropin ( α -MSH) in human brain was investigated by radioimmunoassay using an antiserum which recognized h-ACTH