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Featured researches published by W. J. Wales.


Journal of Dairy Science | 2013

Effects of different strategies for feeding supplements on milk production responses in cows grazing a restricted pasture allowance

M.J. Auldist; L. C. Marett; J.S. Greenwood; M.C. Hannah; J. L. Jacobs; W. J. Wales

Milk production responses of grazing cows offered supplements in different ways were measured. Holstein-Friesian cows, averaging 227 d in milk, were allocated into 6 groups of 36, with 2 groups randomly assigned to each of 3 feeding strategies: (1) cows grazed perennial ryegrass pasture supplemented with milled barley grain fed in the milking parlor and pasture silage offered in the paddock (control); (2) same pasture and allotment supplemented with the same amounts of milled barley grain and pasture silage, but presented as a mixed ration after each milking (PMR 1); and (3) same pasture and allotment, supplemented with a mixed ration of milled barley grain, alfalfa hay, corn silage, and crushed corn grain (PMR 2). For all strategies, supplements provided the same metabolizable energy and grain:forage ratio. [75:25, dry matter (DM) basis]. Each group of 36 cows was further allocated into 4 groups of 9, which were assigned to receive 6, 8, 10, or 12 kg of supplement DM/cow per day. Thus, there were 2 replicated groups per supplement amount per dietary strategy. The experiment had a 14-d adaptation period and an 11-d measurement period. Pasture allotment was approximately 14 kg of DM/d for all cows and was offered in addition to the supplement. Positive quadratic responses to increasing amounts of supplement were observed for yield of milk, energy-corrected milk (ECM), and fat and protein, and positive linear responses for concentrations of fat and protein for cows on all 3 supplement feeding strategies. No difference existed between feeding strategy groups in yield of milk, ECM, or protein at any amount of supplement offered, but yield and concentration of fat was higher in PMR 2 cows compared with control and PMR 1 cows at the highest amounts of supplementation. Responses in marginal ECM production per additional kilogram of supplement were also greater for PMR 2 than control and PMR 1 cows when large amounts of supplement were consumed. For all diets, marked daily variation occurred in ruminal fluid volatile fatty acids and pH, especially in cows fed the largest amounts of supplement. It was concluded that when supplements are fed to grazing dairy cows, a simple mix of grain and pasture silage has no benefit over traditional strategies of feeding grain in the parlor and forage in the paddock. However, yield of milk fat and marginal milk production responses can be greater if the strategy uses an isoenergetic ration that also contains alfalfa hay, corn silage, and corn grain.


Animal Production Science | 2013

Replacing wheat with canola meal in a partial mixed ration increases the milk production of cows grazing at a restricted pasture allowance in spring

M.J. Auldist; L. C. Marett; J. S. Greenwood; M. M. Wright; M.C. Hannah; J. L. Jacobs; W. J. Wales

Milk production responses were measured in grazing cows offered supplements in different ways. Holstein–Friesian cows averaging 70 days in milk were allocated into 20 groups of eight, each including one rumen-fistulated cow. One of three dietary treatments was then randomly assigned to each of the 20 groups. Treatments were (1) Control (8 groups), where cows were supplemented with rolled wheat grain fed twice daily in the dairy and pasture silage provided in the paddock; (2) partial mixed ration (PMR; 8 groups), where cows were offered a PMR comprising rolled wheat grain, maize grain, maize silage and lucerne hay, which was presented on a feedpad immediately after each milking; the PMR was formulated to provide the same estimated metabolisable energy intake as the Control supplements; and (3) PMR+Canola (4 groups), where cows were fed in the same way as the PMR cows, except that a proportion of the wheat in the PMR was replaced with solvent-extracted canola meal. This ration was formulated to provide the same metabolisable energy as the Control and PMR treatments, but had greater amounts of crude protein. For Control and PMR treatments, supplements were offered at 8, 10, 12 or 14 kg DM/cow.day (2 groups per amount) while for the PMR+Canola treatment supplement was offered at 12 or 14 kg DM/cow.day (2 groups per amount). In addition to their supplements, all groups grazed an allowance of ~14 kg DM/cow.day (measured to ground level) of perennial ryegrass pasture. Yields of energy-corrected milk increased linearly with increasing supplement intake, but there was no difference between Control and PMR cows. When canola meal was added to the PMR, there was an increase in energy-corrected milk at a predicted supplement intake of 13.0 kg DM/cow.day. This was associated with a greater concentration and yield of milk fat in the PMR+Canola cows. Ruminal fluid pH and DM intake from pasture were also greater in PMR+Canola cows. It is concluded that farmers feeding high amounts of supplements to grazing cows could increase milk production by carefully considering the composition and form of the supplement mix, including the inclusion of canola meal.


Journal of Dairy Science | 2013

Energy partitioning in herbage-fed dairy cows offered supplementary grain during an extended lactation

S.R.O. Williams; T. Clarke; M.C. Hannah; L. C. Marett; Peter J. Moate; M.J. Auldist; W. J. Wales

An experiment was conducted to quantify the changes in energy partitioning resulting from grain supplementation in herbage-fed dairy cows at 4 stages during a 670-d lactation. The experiment used 16 lactating Holstein-Friesian cows, with a control and a grain treatment being randomly allocated to 8 cows each. During 4 measurement periods (each of 4d in a metabolism stall and 3d in an indirect calorimeter) beginning at approximately 110, 270, 450, and 560 d in milk (DIM), the energy balance of each cow was measured. Cows in both groups were individually offered freshly cut ryegrass pasture (Lolium hybridum L.) in periods 1 and 3 and ryegrass pasture silage and alfalfa (Medicago sativa L.) hay in periods 2 and 4. In all periods, cows in the grain group were offered an additional 4.4 to 5.0 kg of dry matter of cereal grain/cow per day. Adding grain to the diet increased yields of fat and protein and tended to increase yields of milk and lactose, but did not affect milk composition. Gross energy intake (GEI) declined as lactation progressed. Adding grain to the diet decreased the percentage of GEI in feces and urine, but the extent of these reductions did not change as lactation progressed. Adding grain to the diet similarly reduced the percentage of GEI lost to heat, but again the extent of the reduction remained similar as lactation progressed. The magnitude of the increase in milk energy resulting from grain supplementation did not change with advancing lactation, but tissue energy retention was greater in the first 300 DIM compared with after 300 DIM. For herbage-based diets, CH(4) emissions ranged from 6.2 to 7.6% of GEI, which corresponds to 24.0 to 25.8 g of CH(4)/kg of dry matter intake. For diets supplemented with cereal grains, CH(4) emissions ranged from 6.3 to 7.3% of GEI, which corresponds to 21.6 to 25.2 g of CH(4)/kg of dry matter intake. It was concluded that, for cows producing <24 kg of milk/d and consuming herbage-based diets supplemented with grain, the efficiency of utilizing the additional energy in the grain, as measured by the loss of energy in heat, and its partitioning to milk, did not change as lactation progressed from 110 to 560 DIM.


Animal Production Science | 2006

Profitable feeding of dairy cows on irrigated dairy farms in northern Victoria

W. J. Wales; J. W. Heard; C. K. M. Ho; Clare M. Leddin; C. R. Stockdale; G. P. Walker; P. T. Doyle

Milk production per cow and per farm in the irrigated region in northern Victoria have increased dramatically over the past 2 decades. However, these increases have involved large increases in inputs, and average productivity gains on farms have been modest. Before the early 1980s, cows were fed predominantly pasture and conserved fodder. There is now large diversity in feeding systems and feed costs comprise 40–65% of total costs on irrigated dairy farms. This diversity in feeding systems has increased the need to understand the nutrient requirements of dairy cows and the unique aspects of nutrient intake and digestion in cows at grazing. Principles of nutrient intake and supply to the grazing dairy cow from the past 15 years’ research in northern Victoria are summarised and gaps in knowledge for making future productivity gains are identified. Moreover, since the majority of the milk produced in south-eastern Australia is used in the manufacture of products for export, dairy companies have increased their interest in value-added dairy products that better meet nutritional requirements or provide health benefits for humans. Finally, some examples of the impacts of farm system changes on operating profit for some case study farms in northern Victoria are presented to illustrate the need for thorough analysis of such management decisions.


Food Chemistry | 2013

Ultra-high-performance liquid chromatography-ion trap mass spectrometry characterisation of milk polar lipids from dairy cows fed different diets.

V. Craige Trenerry; Ghazal Akbaridoust; Tim Plozza; Simone Rochfort; W. J. Wales; Martin J. Auldist; Said Ajlouni

Milk polar lipids are an important class of biologically active species for human health and for improving the physical functionality of food ingredients. Milk polar lipids from 144 multiparous Holstein-Friesian dairy cows fed different diets were analysed using ultra-high-performance liquid chromatography-ion trap mass spectrometry (UHPLC-MS(n)). A complex profile of polar lipids, consisting of 7 species of phosphatidylinositol (PI), 12 species of phosphatidylethanolamine (PE), 18 species of phosphatidylcholine (PC) and 13 species of sphingomyelin (SM) were identified from the molecular ions and sequential MS(n) fragmentation. Qualitative assessment of the data suggested that different cow diets influenced the relative amounts of a small number of species in the milk samples, e.g. PE 14:0/18:1, PE 18:0/18:1, PC 15:0/18:1, PC 18:0/18:1, SM d18:1/14:0, SM d18:1/15:0, SM d18:1/22:0 and SM d18:1/23:0.


Animal Production Science | 2013

Use of partial mixed rations in pasture-based dairying in temperate regions of Australia

W. J. Wales; L. C. Marett; J. S. Greenwood; M. M. Wright; J. B. Thornhill; J. L. Jacobs; C. K. M. Ho; M.J. Auldist

There is a growing diversity and complexity of dairy farming systems in Australia. Feeding systems based on the provision of mixed rations to dairy cows grazing perennial pastures (termed partial mixed rations or PMR systems) have emerged and present the dairy industry with opportunities for improved production and feed efficiency, but also with significant challenges. Early research results are beginning to define the situations in which PMR systems are profitable and the mechanisms responsible for the improved milk responses. This review focuses on the role of PMR feeding systems in temperate dairying regions of Australia, highlights initial research findings, and identifies some of the gaps in current knowledge that warrant further research. The key findings were that, when very low allowances of pasture are offered to cows, milk production responses were driven mostly by increases in dry matter (DM) intake, and there appeared to be a minimal contribution to increased energy supply from improved whole tract DM digestibility. Differences in milk responses became apparent when >10 kg of total supplement DM was consumed (0.75u2009:u20090.25 concentrate to forage) as PMR. There was a consistent maintenance of milk fat concentration when increasing amounts of concentrates were consumed as PMR, in contrast with supplements consumed in the dairy. There was also a consistent finding that replacing some wheat in the PMR with canola meal resulted in cows consuming more grazed pasture despite the limitations of very low pasture allowances (10–15 kg DM/cow.day, expressed to ground level). This was accompanied by further increases in energy-corrected milk yield. The potential to improve DM intake was further highlighted when pasture allowance was increased, with intake increasing from 3.6% to 4.5% of liveweight (from 20 to 25 kg DM/day for a 550-kg cow). There was also an indication that ~50% of the milk production benefit from PMR can be captured by providing the concentrate supplement as a grain mix in the dairy. There did not appear to be negative impacts of PMR systems on the social and grazing behaviour or health of cows.


Animal Production Science | 2014

Methane emissions of dairy cows cannot be predicted by the concentrations of C8:0 and total C18 fatty acids in milk

S. R. O. Williams; Peter J. Moate; M. H. Deighton; M.C. Hannah; W. J. Wales

Methane (CH4) emissions from dairy cows are technically difficult and expensive to measure. Recently, some researchers have found correlations between the concentrations of specific fatty acids in milk fat and the CH4 emissions from cows that could obviate the need for direct measurement. In this research, data on individual cow CH4 emissions and concentration of caprylic acid (C8:0) and total C18 fatty acids in milk were collated from eight experiments involving 27 forage-based diets and 246 Holstein-Friesian dairy cows. Linear regressions between CH4 and both C8:0 and total C18 in milk were produced for published data and used to calculate 95% prediction regions for a new observation. The proportion of observed methane emissions from eight experiments that fell outside the 95% prediction region was 27.6% for the C8:0 model and 26.3% for the total C18 model. Neither model predicted CH4 emission well with Lin’s coefficient of concordance of less than 0.4 and the Nash–Sutcliffe efficiency coefficient of approximately zero for both the C8:0 and total C18 models. In addition, general linear model analysis showed significant differences between experiments in their intercepts (P < 0.001) and slopes (P < 0.001). It is concluded that the relationships tested cannot be used to accurately predict CH4 emissions when cows are fed a wide range of diets.


Animal Production Science | 2011

Calculating dry matter consumption of dairy herds in Australia: the need to fully account for energy requirements and issues with estimating energy supply

J. W. Heard; P. T. Doyle; S. A. Francis; M. v. H. Staines; W. J. Wales

Feed costs are the major component of the variable costs and a significant component of the total costs of milk production on Australian dairy farms. To improve farm productivity, farmers need to understand how much feed is being consumed and the nutritive characteristics of the diet. This paper reviews an existing simple approach, the ‘Target 10’ approach, which is commonly used by the dairy industry in Victoria to estimate annual forage consumption. An alternative approach – the ‘Feeding Systems’ approach – is then introduced. The ‘Feeding Systems’ approach is compared with estimated forage consumption measured under experimental conditions. An analysis of the sensitivity of both approaches to incremental changes in key variables is presented. The ‘Feeding Standards’ approach was concordant with estimated forage consumption measured under experimental conditions. Sensitivity analysis has highlighted key variables which may have considerable influence over simulated forage consumption using this approach. Given that none of the key variables tested in this analysis can be varied in the ‘Target 10’ approach, we feel confident that the ‘Feeding Standards’ approach provides an improved method of back-calculating annual on-farm forage consumption. Using a robust approach to calculate forage consumption which fully accounts for metabolisable energy requirements is important where farmers are using home-grown forage consumption as an indicator of farm feeding system performance. It is also important to understand the assumptions involved in estimating metabolisable energy supply from either supplements or forage.


Journal of Dairy Science | 2015

Response of plasma glucose, insulin, and nonesterified fatty acids to intravenous glucose tolerance tests in dairy cows during a 670-day lactation

L. C. Marett; M.J. Auldist; Peter J. Moate; W. J. Wales; K.L. Macmillan; F. R. Dunshea; B. J. Leury

This experiment investigated the metabolic response of dairy cows undergoing an extended lactation to a frequently sampled intravenous glucose tolerance test. The experiment used 12 multiparous Holstein cows that calved in late winter in a seasonally calving pasture-based system and were managed for a 670-d lactation by delaying rebreeding. In each of four 5-wk experimental periods commencing at approximately 73, 217, 422, and 520 (±9.1) days in milk (DIM), cows were offered a diet of perennial ryegrass (73 and 422 DIM) or pasture hay and silage (217 and 520 DIM) supplemented with 1kg of DM grain (control; CON) or 6kg of DM grain (GRN) as a ration. Daily energy intake was approximately 160 and 215 MJ of metabolizable energy/cow for the CON and GRN treatments, respectively. At all other times, cows were managed as a single herd and grazed pasture supplemented with grain to an estimated minimum daily total intake of 180 MJ of metabolizable energy/cow. Cows were fitted with an indwelling jugular catheter during the final week of each experimental period. The standard intravenous glucose tolerance test using 0.3g of glucose per kilogram of body weight was performed on each cow at approximately 100, 250, 460, and 560 DIM. Plasma concentrations of glucose, insulin, and nonesterified fatty acids (NEFA) responses were measured. Milk yield, milk solids yield, body weight, and basal plasma glucose were greater in the GRN compared with the CON treatment. The area under the plasma response curve relative to baseline (AUC) for glucose, insulin, and NEFA and their apparent fractional clearance rates indicated varied whole body responsiveness to insulin in terms of glucose metabolism throughout the 670-d lactation. The glucose AUC 0 to 20 min postinfusion was increased at 560 DIM, indicating reduced utilization of glucose by the mammary gland at this stage of lactation. The NEFA clearance rate, 6 to 30 min postinfusion, was greater at 460 and 560 DIM. These data indicated an increase in lipogenic activity or a decrease in lipolysis as lactation progressed, suggestive of an overall increase in responsiveness to insulin in terms of whole body lipid metabolism as lactation progressed. These observations are consistent with decreased priority of lactation beyond 300 DIM. Cows in the GRN treatment had decreased whole body responsiveness to hyperglycemia compared with CON cows in terms of glucose clearance and AUC for the glucose response. Variation in the response curves of plasma glucose, NEFA, and insulin was predominantly a result of stage of lactation and not diet. This may be due to changes in mammary gland uptake of glucose that is independent of insulin and the responsiveness of peripheral tissues to the actions of insulin at different stages of the lactation that are independent of diet.


Journal of Dairy Science | 2016

Milk production responses to different strategies for feeding supplements to grazing dairy cows

M.J. Auldist; L. C. Marett; J.S. Greenwood; M. M. Wright; M.C. Hannah; J. L. Jacobs; W. J. Wales

Milk production responses of grazing cows offered supplements in different ways were measured. Holstein-Friesian cows, averaging 45 d in milk, were allocated into 8 groups of 24, with 2 groups randomly assigned to each of 4 feeding strategies. These were control: cows grazed a restricted allowance of perennial ryegrass pasture supplemented with milled wheat grain fed in the milking parlor and alfalfa hay offered in the paddock; FGM: same pasture and allowance as the control supplemented with a formulated grain mix containing wheat grain, corn grain, and canola meal fed in the parlor and alfalfa hay fed in the paddock; PMRL: same pasture and allowance as the control, supplemented with a PMR consisting of the same FGM but mixed with alfalfa hay and presented on a feed pad after each milking; and PMRH: same PMR fed in the same way as PMRL but with a higher pasture allowance. For all strategies, supplements provided the same metabolizable energy and grain:forage ratio [75:25, dry matter (DM) basis]. Each group of 24 cows was further allocated into 4 groups of 6, which were randomly assigned to receive 8, 12, 14, or 16 kg of DM supplement/cow per d. Thus, 2 replicated groups per supplement amount per dietary strategy were used. The experiment had a 14-d adaptation period and a 14-d measurement period. Pasture allowance, measured to ground level, was approximately 14 kg of DM/d for control, FGM, and PMRL cows, and 28 kg of DM/d for the PMRH cows, and was offered in addition to the supplement. Positive linear responses to increasing amounts of supplement were observed for yield of milk, energy-corrected milk, fat, and protein for cows on all 4 supplement feeding strategies. Production of energy-corrected milk was greatest for PMRH cows, intermediate for FGM and PMRL cows, and lowest for control cows. Some of these differences in milk production related to differences in intake of pasture and supplement. Milk fat concentration decreased with increasing amount of supplement for all feeding strategies, but the decline was most marked for the control cows. Milk protein concentration increased for all groups as the amount of supplement increased, but was greater for FGM, PMRL, and PMRH cows than control cows. It is concluded that when supplements are fed to grazing dairy cows, inclusion of corn grain and canola meal can increase milk production even at similar metabolizable energy intakes, and that it does not matter whether these supplements are fed as a PMR or in the parlor and paddock.

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L. C. Marett

Cooperative Research Centre

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J. L. Jacobs

Cooperative Research Centre

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Peter J. Moate

University of Pennsylvania

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M. M. Wright

University of Melbourne

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B. J. Leury

University of Melbourne

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Mf Pyman

University of Melbourne

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