W.M. Davis

Cannabichromene and Δ9-tetrahydrocannabinol: Interactions relative to lethality, hypothermia and hexobarbital hypnosis

Abstract 1. 1. LD50s in mice after single intraperitoneal (i.p.) doses of cannabichromene (CBC) and Δ9-tetrahydrocannabinol (Δ9-THC) were 113.4 and 276.3 mg/kg, respectively. A small dose (25 mg/kg) of CBC given concurrently with Δ9-THC lowered the LD50 of Δ9-THC to 152.0 mg/kg. 2. 2. CBC, like Δ9-THC, caused hypothermia in mice; it reduced the effect of Δ9-THC at early times and increased it at later times after the two were injected simultaneously i.p. 3. 3. CBC and Δ9-THC, in 25 mg/kg i.p. doses, each prolonged hexobarbital hypnosis equally in mice, but had no additive effect in combination.

Perinatal exposure to cannabichromene and Δ9-tetrahydrocannabinol: Separate and combined effects on viability of pups and on male reproductive system at maturity

The effects of cannabichromene (CBC), delta 9-tetrahydrocannabinol (delta 9-THC) and their combination (all doses 50 mg/kg orally) were determined after being administered to female mice for 7 days beginning on the 20th day of gestation. The THC treatment reduced postnatal viability, impaired male reproductive behavior at maturity and significantly reduced seminal vesicle weights. No changes from control values occurred after CBC or CBC + THC. Thus, CBC alone at this dosage did not act like THC; moreover, it antagonized the effects of THC when the two were given in combination.

Synergism of cannabichromene and CNS depressants in mice

Abstract 1. 1. One intraperitoneal dose of the cannabinoid, cannabichromene (CBC), increased the duration of loss of righting reflex in mice after hexobarbital, barbital or zoxazolamine. 2. 2. After 7 daily doses of CBC, prolongation of response to the 3 drugs no longer occurred; duration for hexobarbital and zoxazolamine was less than for vehicle controls. 3. 3. The data reflect a CNS depressant action of CBC, the development of tolerance to this action, and a possible induction of drug metabolism.

Comparison of stimulants and hallucinogens on shuttle avoidance in rats

Rats were trained to a high level of performance of a conditioned avoidance response in a shuttlebox to test effects of several classical stimulants in comparison to a variety of hallucinogens. A previously-reported biphasic pattern of effects of mescaline on shuttle avoidance was replicated and extended to 12 other hallucinogens of both phenylethylamine and indolealkylamine classes. Response patterns of hallucinogens could be differentiated from 3 stimulants and from a methoxyamphetamine compound that lacks hallucinogenic activity.

Effects of an i.v. lethal dose of 3,4-methylenedioxyamphetamine (MDA) in the dog and antagonism by chlorpromazine

A high intravenous (i.v.) dose of MDA (20 mg/kg) to mongrel dogs elevated body temperature, heart rate, mean arterial pressure and other cardiovascular parameters initially, but only the 1st two remained high. Other functions soon became quite depressed, and death shortly ensued. Arterial pO2 decreased, but pH and pCO2 showed a biphasic response after an initial decrease, Dogs that received chlorpromazine (10 mg/kg, i.v.) after MDA showed stabilization of physiological parameters, and survival through 48 hr.