W.M.L. Molhoek
Wageningen University and Research Centre
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Featured researches published by W.M.L. Molhoek.
European Journal of Plant Pathology | 1995
J. Köhl; W.M.L. Molhoek; Carin H. van der Plas; N.J. Fokkema
In this study, the hypothesis was tested that removal of substrate for sporulation ofBotrytis spp. may lead to a retardation of an epidemic if the majority of the inoculum is produced inside the treated crop. Suppression of sporulation ofBotrytis spp. could be an attractive option for biological control ofBotrytis leaf spot in onions. In a field experiment, necrotic leaf tissue was removed to simulate the effect of a biocontrol agent. By this means, the amount of substrate on whichBotrytis spp. sporulates was reduced. In the experiment, the spore load above the onion plots was significantly reduced and the epidemic of onion leaf spot was retarded. At the end of the growing season, the number of leaf lesions in the green leaf area was lower in plots with substrate removal than in control plots (0.6 and 1.1 cm−2, respectively). The results demonstrated that an epidemic of onion leaf spot largely depends on the rate of inoculum production inside a crop. Thus, suppression of sporulation on necrotic leaf tissue is a valid control strategy that could be applied by using sporulation suppressing antagonists.
European Journal of Plant Pathology | 1995
J. Köhl; C. H. van der Plas; W.M.L. Molhoek; N.J. Fokkema
Saprophytic antagonists were evaluated for suppression of sporulation ofBotrytis allii andB. cinerea on artificially killed segments of onion leaves that were pre-inoculated with the pathogens. During incubation of the antagonisttreated leaf segments in moist chambers, periods of leaf wetness and leaf dryness were alternated to simulate conditions in the field. Interruption of humid conditions with dry periods had a differential effect on antagonists.Alternaria alternata, Chaetomium globosum, Ulocladium atrum andU. chartarum suppressed sporulation ofB. allii almost completely under continuously wet conditions, and when the leaf wetness periods were interrupted with drying periods of 9h imposed 16, 40, and 64 h after the antagonists were applied. When leaf wetness was interrupted 16 h after antagonist application, the number of conidia ofB. allii produced cm−2 leaf surface after eight days was under the detection limit of 5.2 × 103 conidia on leaves treated with these antagonists compared to 3.7 × 105 conidia on leaves that were not treated. On the other hand,Gliocladium roseum, G. catenulatum andSesquicillium candelabrum, all highly efficient under continuously wet conditions, were of low to moderate efficiency when leaf wetness periods had been interrupted 16 h after application of the antagonists. The antagonists showed the same differentiation and sensitivity to interrupted wetness periods when tested withB. cinerea.
Plant Disease | 2015
J. Köhl; Christian Scheer; Imre Holb; Sylwester Masny; W.M.L. Molhoek
Apple scab, caused by Venturia inaequalis, is the most important disease in apple production, reducing yield and quality of fruit. Control of apple scab in commercial orchards currently depends on multiple applications of fungicides. The potential of the antagonistic isolate Cladosporium cladosporioides H39, originating from a sporulating colony of V. inaequalis, to control apple scab development was tested in eight trials during 2 years in orchards in Eperjeske (Hungary), Dabrowice (Poland), and Bavendorf (Germany) planted with different cultivars. Treatments were conducted as calendar sprays or after infection periods. Additional trials in an orchard in Randwijk (The Netherlands) focused on the effect of timing of antagonist application before or after infection periods. The overall results of the field trials consistently showed-for the first time-that stand-alone applications of the antagonist C. cladosporioides H39 can reduce apple scab in leaves and fruit. This was demonstrated in an organic growing system as well as in conventional orchards by spray schedules applied during the primary or the summer season. In both systems, the same control levels could be reached as with common fungicide schedules. Efficacies reached 42 to 98% on leaf scab incidence and 41 to 94% on fruit scab. The antagonist was also effective if applied one or even several days (equivalent to approximately 300 to 2,000 degree h) after infection events in several field trials and a trial conducted in Randwijk with single-spray applications at different intervals before or after infection events. Better understanding of the biology of the antagonist will help to further exploit its use in apple scab control.
Phytopathology | 1995
J. Köhl; W.M.L. Molhoek; C. H. Van Der Plas; N.J. Fokkema
European Journal of Plant Pathology | 2009
J. Köhl; W.M.L. Molhoek; Belia H. Groenenboom-de Haas; Helen H. M. Goossen-van de Geijn
Ecofruit. 13th International Conference on Cultivation Technique and Phytopathological Problems in Organic Fruit-Growing. Proceedings of the conference, Weinsberg, Germany, 18-20 February 2008 | 2008
J. Köhl; W.M.L. Molhoek; L. Groenenboom-de Haas; H.M. Goossen-van de Geijn; U. Eiben
In: M. Manka (ed.). Environmental biotic factors in integrated plant disease control. The Polish Phytopathological Society, Poznan, pp. 309-311 | 1995
J. Köhl; W.M.L. Molhoek; C.H. van der Plas; N.J. Fokkema
Archive | 2008
J. Köhl; W.M.L. Molhoek; L. Groenenboom-de Haas; H.M. Goossen-van de Geijn; U. Eiben
Archive | 2008
J. Köhl; W.M.L. Molhoek; B.H. de Haas; H.M. Goossen-van de Geijn
Archive | 1995
J. Köhl; W.M.L. Molhoek; C.H. van der Plas; N.J. Fokkema; M. Gerlagh