W.R. Breg
Yale University
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Featured researches published by W.R. Breg.
Cytogenetic and Genome Research | 1963
O.J. Miller; B.B. Mukherjee; W.R. Breg; A. Van N. Gamble
Flattened metaphase figures were obtained by squashing or air-drying cells from human leucocyte cultures that had been treated with colchicine and hypotonic sodium citrate prior to fixation. In such metaphase figures the Y chromosome was at the periphery of the figure more often than chance expectation and more often than the chromosome pairs 1, 2, or 16 or the chromosome groups 13–15, 17–18, 19–20, or 21–22. The Y chromosome was further from the center of circular metaphase figures than the mean value of all the chromosomes or the chromosome pairs 1, 2, or 3 or the chromosome groups 4–5, 6–12+X, 19–20 or 21–22.
Cytogenetic and Genome Research | 1963
O.J. Miller; W.R. Breg; B.B. Mukherjee; A. Van N. Gamble; A.C. Christakos
Two collections of XY-containing karyotypes were screened to obtain every karyotype in which the Y chromosome was distinguishable from the other small acrocentric chromosomes. The metaphase figures co
Cytogenetic and Genome Research | 1973
Elaine H. Zackai; W.R. Breg
Two males with apparently identical rings of chromosome 7 and very different phenotypic features are presented. Patient 1 is severely retarded, has short stature, microcephaly, craniosynostosis, unilateral proptosis, ptosis, and microcornea, small phallus, first-degree hypospadias, and undescended testes. Patient 2 is of normal intelligence, and his only abnormalities are short stature and a small head. Study of lymphocytes at metaphase showed the same proportion of cells with the ring, 46, XY, r(7), and those without a ring, 45, XY,-7, in each case. Quinacrine fluorescence and Giemsa banding techniques showed all the bands of chromosome 7 to be present, suggesting little material missing as a result of ring formation. A similar degree of instability of the ring in each case was indicated by the frequency of abnormal cells at anaphase and telophase.
Cytogenetic and Genome Research | 1984
M. Rabin; M. Watson; P.E. Barker; J. Ryan; W.R. Breg; Frank H. Ruddle
The human transforming gene NRAS, cloned from SK-N-SH neuroblastoma cells, has been mapped to region p11----p13 on chromosome 1 by in situ hybridization.
Cytogenetic and Genome Research | 1966
M.W. Steele; W.R. Breg; A.I. Eidelman; D.T. Lion; T.A. Terzakis
An instance of a B-group ring chromosome with a unique mosaic pattern occurring in a newborn with cri du chat syndrome is described in detail. The patient initially demonstrated mosaicism with 98% of cells having 45 chromosomes and one ring and 2% having 46 chromosomes and no ring. Reasons for this mosaic pattern are discussed. The ring chromosome seemed stable in size but decreased in frequency as the patient aged. Autoradiographic studies on this ring chromosome, as reported by Miller et al. (1966), did not clearly indicate whether it was one of the late or early replicating B chromosomes. Dermatoglyphic and blood grouping studies are presented.
Experimental Cell Research | 1964
B.B. Mukherjee; O.J. Miller; W.R. Breg; S. Bader
Abstract Patterns of chromosome replication in cultured leucocytes from a presumptive XXX and a presumptive XXXXY human subject have been studied by the method of autoradiography. Leucocyte cultures were exposed to tritiated thymidine and colchicine for 8–9 hr and harvested directly from the isotope-containing medium. Ambiguity in classification of individual chromosomes due to overlying grains was overcome by taking two photographs of each cell, one before applying the film and the other at the focal plane of the overlying grains. The characteristic pattern of DNA replication found in the cultured leucocytes from the XXX female were indistinguishable from that seen in leucocytes from the XXXXY male. This pattern was also indistinguishable from the DNA replication pattern seen in normal females. One of the chromosomes in group 19–20 was the first chromosome in the whole complement to terminate replication, followed first by one chromosome each in group 17–18 and group 21–22, and then by a single chromosome in group 13–15. Many other examples of homologue asynchrony were noted, one of the most striking involving the X chromosomes. Two chromosomes in group 6–12 + X, presumably X chromosomes, in metaphase figures from the presumptive XXX female and three chromosomes of the same group in metaphase figures from the presumptive XXXXY male were the last chromosomes in the whole complement to complete DNA synthesis. One homologue of chromosome 3 and one chromosome each in group 4–5 and 13–15 also terminated replication very late and only before the late-replicating presumptive X chromosomes. The late-replicating presumptive X chromosomes were at the periphery of the metaphase figures more often than would be expected by chance. They showed variation in both length and arm ratios, even within individual metaphase figures.
Cytogenetic and Genome Research | 1976
Gloria C. Koo; Stephen S. Wachtel; W.R. Breg; O.J. Miller
Cytogenetic and Genome Research | 1976
W.R. Breg; M.M. Aronson; R. Hill; A.E. Greene; L.L. Coriell
Cytogenetic and Genome Research | 1977
W.R. Breg; M.M. Aronson; A.E. Greene; L.L. Coriell
Cytogenetic and Genome Research | 1976
W.R. Breg; M.M. Aronson; A.E. Greene; L.L. Coriell