Walter Leira Teixeira Filho

Contribution on the study of Isospora hemidactyli Carini, 1936 and a report of an adeleid pseudoparasite of the house gecko Hemidactylus mabouia, from the Rio de Janeiro Metropolitan Region, Brazil

A description of the coccidium Isospora hemidactyli from the house gecko Hemidactylus mabouia, a very common at dwellings in Rio de Janeiro Metropolitan Region, was made in this study. Histograms and linear regression were made for this species and determined the homogeneity of these oocysts despite of large range. Besides it, polysporocystid oocysts also were recovered from feces of the H. mabouia house gecko and they were similar to those described previously as the genus Adelina. This species should be parasitizing an invertebrate ingested by house gecko, and for that reason, is a pseudoparasite. Oocysts of I. hemidactyli were subspherical to ellipsoidal, 24.4 x 22.3 microm, with single-layered wall and one polar granule. Sporocysts were subspherical to ellipsoidal, 11.8 x 9.8 microm with Stieda and substieda bodies, residuum and sporozoites with refractile body. Oocysts of the pseudoparasite Adelina sp. were ellipsoidal, 36.3 x 30.9 microm, with bi-layered wall and without micropyle, residuum and polar granule. Eight to 15 sporocysts were presents and were subspherical to broadly ellipsoidal, 12.4 x 11.2 microm. Stieda and substieda bodies were absent. Sporozoites present refractile bodies at both ends.

AFLATOXIN EFFETC ON THE OOCYSTS MORPHOMETRY AND CONTRIBUTION ON THE MORPHOLOGY OF Eimeria bateri BHATIA, PANDEY AND PANDE, 1965 FROM THE JAPANESE QUAIL Coturnix japonica IN BRAZIL

The purpose of this study was to characterize Eimeria bateri oocysts and to evaluate the aflatoxin effect in the morphometry of sporulated oocysts in Japanese quails infected naturally. Of a total of 50 quails naturally infected by E. bateri were randomly divided into two groups with 25 birds each. In one of them, quails were orally administered with aflatoxin in dose of 0.04 mg/kg body weight previously. Both experimental groups shed E. bateri oocysts. These oocysts were subspherical to ellipsoidal, 25.1 x 18.9 microm, with bi-layered wall. Micropyle and residuum were absent, but one or more polar granules were present. Sporocysts elongate ovoid, 12.5 x 7.4 microm. Stieda and substieda bodies were present. Sporocyst residuum was dispersed and sporozoites presented a nucleus and a refractile body. Histograms confirmed the presence of a single species, E. bateri. Linear regression proved that E. bateri oocysts are polymorphic, due, basically, to shape of these oocysts. The comparative morphometry between two experimental groups demonstrated that the aflatoxin influenced significantly in the E. bateri oocysts.

Infiltração de leucócitos e imunorreatividade antimieloperoxidase em granulócitos da mucosa e submucosa do intestino grosso de equinos submetidos à sobrecarga dietética com amido

This study was carried out to evaluate leukocyte infiltration and anti-myeloperoxidase immunoreactivity in granulocytes of the mucosa and submucosa of the large intestine of horses submitted to dietetic starch overload. Eight adult horses were allocated randomly in three treatments: Treatment I (Control) (n = 2), animals euthanized without starch overload; and Treatments II (n = 3) and III (n = 3), animals undergoing starch overload, with gastric infusion of 17.6 g starch per kg of body weight, euthanized after 24 and 36 hours, respectively. Only afflux of neutrophils in the intestinal mucosa and submucosa blood vessels (leukocyte stasis) was observed. Eosinophils were the predominant cells in the mucosa and submucosa in all horses, independent of dietetic overload, with infiltration grade from mild to moderate. Lymphocyte infiltration was also observed in all horses, but with lower intensity when compared to eosinophils. Congestion, edema and dilatation of lymphatic vessels were the main circulatory alterations observed, with more intensity in the submucosa. Higher immunoreactivity to the anti-myeloperoxidase antibodies was observed in the mucosa and submucosa of horses 36 hours after overload. Horses submitted to dietetic starch overload showed intestinal inflammatory response with prevalence of eosinophils, leukocyte stasis and circulatory alterations, varying from discreet to moderate.

Eimeria ninakohlyakimovae (Apicomplexa: Eimeriidae) de caprinos leiteiros de um criatório na microrregião do Rio de Janeiro: aspectos morfobiológicos

Com o objetivo de determinar a presenca de Eimeria ninakohlyakimovae, assim como os aspectos morfobiologicos relacionadosa essa especie nos caprinos leiteiros de um criatorio na microrregiao do Rio de Janeiro, utilizou-se um sistema decriacao semi-intensivo localizado no municipio de Marica. Foram formados dois grupos de animais portadores de infeccaonatural: 65 jovens com ate 180 dias de idade e 314 adultos. Foram coletadas 379 amostras individuais de fezes para verificara presenca de oocistos dessa especie e o tempo de esporulacao foi observado nas temperaturas de 20,33± 1 ,24 (temperaturaambiente); 18, 27 e 32°C. Observou-se pleomorfismo dessa amostra de E. ninakohlyakimovae e o tempo de esporulacaodos oocistos foi menor nas temperaturas mais elevadas.