Wanda Moscalewski Abrahão

Occurrence of Listeria monocytogenes in cheese and ice cream produced in the State of Paraná, Brazil

The occurrence of Listeria monocytogenes in Brazilian ice cream and in some soft and semi-soft cheeses produced and sold in the State of Parana, Brazil was evaluated. Ninety samples of cheese and sixty samples of ice creams were analyzed following the guidelines outlined by the official institutes, AOAC and FDA. In the ice cream samples no isolation of Listeria spp. was found. The percentage of these ninety samples of cheeses positive for Listeria spp. was 12.20%. Listeria monocytogenes was detected in six (6.70%) of the same samples. The presence of Listeria innocua was five (5.50%) in the samples analyzed was also observed. According to the results of the work it is possible to presume that there is a potential health risk to the brazilian population, heightened by aging and the increase in immunodepressed. These results indicate the need for the implementation of monitoring of these microorganisms as much by producers as by health inspectors. The results also show that the VIP (visual immunoprecipitation assay) is a viable triage method of contaminated samples for the liberation of products for commercialization, as it is quick, reliable and does not require additional equipment other than that normally found in production labs, while presenting reliable results.

Controle microbiológico em valvas cardíacas humanas

OBJECTIVE To evaluate, from microbiological point of view, the valves processed by Human Heart Valve Bank of Santa Casa de Misericórdia of Curitiba for use in cardiovascular surgeries. METHODS The processing of 1,671 valves, accomplished within the period of time between July 1999 and June 2004, was evaluated. Out of the valves and the solutions involved in the process, samples were collected and spread in culture mediums, such as fluid thioglycollate medium, tryptic soy broth and Sabouraud broth, for incubation during 14 days, using a modified methodology based on the Farmacopéia Brasileira 1988 (Brazilian Pharmacopeia) and USP 1990 (United States Pharmacopeia). The samples in which growing was observed were submitted to microbian identification. RESULTS In a set of 1,671 samples, 92% were considered proper for use under microbiological point of view, since they did not display microbian contamination. The remaining 8% were rejected for clinical use because of contamination in some stage of the valve processing. CONCLUSION From the Analysis of the results, it was observed the importance of microbiological control in human grafts, in order to avoid using microbiologically contaminated valves in patients submitted to cardiovascular surgery.

Evaluation of antimicrobial resistance of Campylobacter spp. isolated from broiler carcasses

Abstract 1. The aim of the present study was to evaluate the antimicrobial resistance of Campylobacter strains (C. jejuni, C. coli and C. lari) isolated from broiler carcasses processed in the State of Paraná, Brazil. 2. Rates of microbial resistance and susceptibility were assessed by both Disk Diffusion (DD) and Etest (Minimum Inhibitory Concentration) techniques. Antibiotics were tested using DD (12 antibiotics) and/or MIC (7 antibiotics) methods. 3. A total of 95.8% of the strains were resistant to at least two agents. In terms of multidrug resistance, 75% of strains were resistant to three or more groups of antibiotics. The highest rates of resistance were detected for cefalotin, ciprofloxacin, tetracycline and nalidixic acid. A high rate of susceptibility of the strains to erythromycin (95.8%) was found confirming that this is considered the agent of choice for treating campylobacteriosis. Comparison of the microbial resistance and susceptibility, as determined simultaneously by the two methods, found the techniques to be statistically equivalent for 5 out of the 6 antibiotics tested. 4. The results of this study suggest the need for adopting measures to control the use of antibiotics in broiler production to prevent multidrug resistance of Campylobacter strains and reduce the risk of serious human diseases caused by the consumption of contaminated chicken meat.

Enzyme-linked imunoassays for the detection of Listeria sp. and Salmonella sp. in sausage: a comparison with conventional methods

This study was carried out comparing the conventional methods (ISO 11290-1 and BAM method, 2008) and system mini-Vidas® (Biomerieux), for detection of Listeria sp. and Salmonella sp. in cooled sausage. The immunoenzymatic method has shown to be effective for the detection of target pathogens, it has presented itself as an excellent screening method.

Detection of thermophilic Campylobacter sp. in raw chicken sausages by methods ISO 10272: 2006 in Curitiba - Parana State - Brazil

The aim of this study was the detection of Campylobacter sp. in raw chicken sausages using the methods ISO 10272-1 and ISO 10272-2. The overall prevalence of Campylobacter sp. in the samples tested was 16.67%, representing a serious risk to the health of consumers, particularly if measures guaranteeing proper cooking of foods and prevention of cross-contamination are not adopted. Furthermore, the majority of campylobacteriosis cases in humans are caused by consumption or improper handling of contaminated raw or undercooked poultry meat, which constitute the main vehicle of this infection.

Comparison of selective agars recommended by method ISO 11290-1 and chromogenic agars for the isolation of Listeria sp. in refrigerated sausages

Este estudo teve como objetivo a analise da prevalencia de Listeria sp. em linguicas resfriadas e a comparacao dos meios seletivos utilizados no plaqueamento do metodo ISO 11290-1 (Agar PALCAM e Agar Oxford), e agares cromogenicos (Agares Listeria Cromogenico CM 1080 (OCLA) e CM 1084 (ISO)). A frequencia de Listeria sp. foi de 52,9%, sendo que destas, 13,7% corresponderam a L. monocytogenes. A eficacia dos quatro agares para o isolamento de L. monocytogenes demonstrou-se satisfatoria. Apesar de haver algumas diferencas nas composicoes dos meios cromogenicos analisados, estas nao pareceram influenciar nas concordâncias entre os resultados expressos. Contudo, o agar PALCAM mostrou-se mais eficaz na supressao de outros micro-organismos, aumentando, assim, a possibilidade de deteccao de especies de Listeria presentes em numero reduzido. Atraves deste trabalho sugere-se a utilizacao do agar PALCAM associado a um meio cromogenico para aumentar a chance de isolamento de cepas atipicas de Listeria sp. em produtos carneos.

Avaliação de diferentes alíquotas de enxaguadura para a contagem pelo método direto de Campylobacter spp. em carcaças de frango resfriadas

The direct method for detecting and enumerating Campylobacter spp. in chicken meat is easy to perform, but the volumes of 100μL and 400μL, as recommended in some methodologies, are often unable to achieve the colonies counts due to the occurrence of confluent growth or microbial contaminants. This study aimed at evaluating the different volumes for rinsing chicken meats in order to minimize the interference of microbial contaminants, without compromising the sensitivity of direct methodology. Rinse volumes of 5, 10, 50, 100 and 400μL were tested using the modified charcoal cefoperazone deoxycholate agar (mCCDA) and modified Bolton agar. The presence of Campylobacter was confirmed by phenotypic methods and PCR assay. However, the strategy of using less than 100 μL of rinse volumes did not improve the isolation and enumeration of Campylobacter colonies, because it reduces the sensitivity of the assay. Thus, a possible solution to minimize the interference of microbial contaminants would be in developing new selective media or incorporate them into other existing antimicrobial drugs.

Genotipagem e Antibiograma de Isolados de Campylobacter Spp. Procedentes de Amostras Clínicas e de Alimentos do Sul do Brasil

Thalyta Marina Benetti, Wanda Moscalewski Abrahao, Tereza Cristina Rocha Moreira Oliveira. Genotipagem e Antibiograma de Isolados de Campylobacter spp. Procedentes de Amostras Clinicas e de Alimentos do Sul do Brasil. In: Anais do 12o Congresso Latinoamericano de Microbiologia e Higiene de Alimentos MICROAL 2014 [= Blucher Food Science Proceedings, num.1, vol.1]. Sao Paulo: Editora Blucher, 2014. DOI 10.5151/foodsci-microal-245 Genotipagem e Antibiograma de Isolados de Campylobacter spp. Procedentes de Amostras Clinicas e de Alimentos do Sul do Brasil

Avaliação da Qualidade Microbiológica de Bolos Cremosos Comercializados em Curitiba, Paraná

O Programa de Puericultura acompanha o crescimento e o desenvolvimento de criancas durante os primeiros anos de vida, estabelecendo condutas preventivas adequadas a idade da crianca, sendo fundamental na prevencao de doencas e no controle da mortalidade infantil. Este trabalho teve como objetivo avaliar o estado nutricional das criancas atendidas no Programa de Puericultura em uma Unidade de Saude do Municipio de Guarapuava-PR. Foram investigados 55 prontuarios de criancas inscritas no Programa para verificacao das variaveis de peso, altura, perimetro cefalico, numero de consultas realizadas, aleitamento materno exclusivo ate os seis meses de idade, alimentacao atual e uso de suplementacao. Os resultados mostraram que a maioria das criancas avaliadas estava eutroficas para os parâmetros nutricionais avaliados, sendo Peso/Idade (85,5%), Peso/Estatura (65,5%), Estatura/Idade (76,4%) e Indice de Massa Corporal/Idade (72,7%). A maioria das criancas fazia uso de suplementacao (67,3%), 38,2% de aleitamento materno complementar e 40% nao receberam leite materno de forma exclusiva ate os seis meses de idade. Destaca-se a importância do acompanhamento do crescimento e desenvolvimento como parte integral a saude da crianca em programas e acoes de promocao a saude e qualidade de vida.O comportamento alimentar dos adolescentes geralmente e vinculado ao consumo de alimentos com alto valor energetico e pobres em nutrientes, com omissao de refeicoes e tendencia as restricoes alimentares, contribuindo, dessa forma, para alteracoes no estado nutricional, o que pode desencadear futuras patologias como a anorexia e bulimia. O objetivo do presente trabalho foi avaliar o efeito da imagem corporal sobre o estado nutricional e comportamento alimentar de adolescentes do sexo feminino, com faixa etaria entre 14 e 17 anos (media de 15,41±1,11 anos), frequentadoras de um Centro Estadual de Educacao Profissional no municipio de Guarapuava-PR. Participaram do estudo 96 adolescentes, regularmente matriculados na Instituicao. Foram analisadas questoes relativas a percepcao da imagem corporal, comportamento alimentar e dados antropometricos. Apos a avaliacao dos resultados, observou-se uma media de altura de 1,62±0,06m, peso de 56,07±5,21kg e IMC de 21,41±2,24kg/m². A maioria das meninas apresentou IMC adequado (84,4%) e nao mostraram riscos para desenvolver transtornos alimentares (72,9%), sendo que a maioria demonstrou distorcao da imagem corporal (52,1%). Torna-se necessario maior atencao em relacao a alimentacao, pois a distorcao da imagem corporal pode levar a pratica de dietas restritivas e desenvolvimento de transtornos alimentares.O aumento da procura de produtos prontos para consumo e considerado uma tendencia atual, principalmente em grandes centros urbanos, refletindo na expansao de estabelecimentos como redes de “fast-food”, padarias, confeitarias e lanchonetes, e aumentado de forma direta a preocupacao pelos orgaos de vigilância na manutencao da qualidade higienico-sanitaria dos produtos comercializados. Este trabalho teve como objetivo avaliar a qualidade microbiologica de bolos cremosos comercializados no municipio de Curitiba-PR e analisar as condicoes higienico-sanitarias as quais os produtos foram expostos durante o processamento e armazenamento. Para a presente pesquisa foram coletadas 14 amostras diferentes de bolos cremosos. Avaliou-se a qualidade microbiologica de cada amostra seguindo recomendacoes contidas na RDC no12, de 02 de janeiro de 2001, ANVISA/MS. Neste estudo constatou-se o nao isolamento de Salmonella em nenhuma das amostras analisadas. Em 14,3% (n=2) das amostras o NMP de coliformes termotolerantes apresentou-se acima do padrao microbiologico vigente. Em 28,6% (n=4) das amostras, observou-se contagens de estafilococos coagulase-positiva acima do limite estabelecido, sendo que 02 amostras eram potencialmente capazes de causar intoxicacao alimentar. Observou-se a incidencia de 14,3% (n=2) de Listeria sp nas das amostras analisadas. O numero mais provavel de coliformes totais acima de 2,17 log/g foi quantificado em 05 amostras (35,7%). Os resultados mostram que ha necessidade de maior controle de qualidade desde a aquisicao da materia-prima ate ao produto acabado, atraves da adocao de Boas Praticas de Fabricacao e o Programa de Analise de Perigos e Pontos Criticos de Controle, de forma a garantir um produto saudavel e seguro para o consumidor.