Wang Zhixiang

Preoperative diagnosis of renal oncocytoma: case report and literature review

Abstract To differentiate renal oncocytoma from renal carcinoma, the clinical data of four patients with incidentally found renal oncocytomas were studied in this report. And additional Immunohistochemistry examinations were done to confirm diagnosis. Renal oncocytomas were found incidentally in four patients during medical examination. No characteristic changes were found in laboratory tests. Radiology examination provided the location and possibility of renal oncocytoma. History, laboratory test and radiology exanimation indicated the diagnosis of renal oncocytoma, but the final identify of renal oncocytoma need pathology examination.

LESS Adrenal Surgery

One-port, single-incision laparoscopy is part of the natural development of minimally invasive surgery. Laparoendoscopic single-site (LESS) surgery has been conceived as a natural evolution to a further reduction of surgical trauma and has been steadily gaining momentum during the past 6 years. The population has a favorable perception of scarless surgery, even in the case of increased procedural risks of complications, in spite of cure and surgical safety being the main concerns. Compared with conventional laparoscopic surgery, LESS adrenal surgery is more difficult. The clear disadvantages of LESS adrenal surgery include instrument congestion around a single port and the inability to achieve adequate triangulation. In this chapter, the LESS adrenalectomy and LESS partial adrenalectomy would be discussed. And particularly in LESS adrenalectomy, the transperitoneal approach and retroperitoneal approach would be represented step by step. The advantages and disadvantages of each transperitoneal approach and retroperitoneal approach during LESS adrenal surgery would be discussed.

RNA干扰 p 38基因对肾癌786-O细胞生物学特性的影响及对舒尼替尼的增敏作用

目的探讨RNA干扰p38基因对人肾癌细胞株786-O增殖、侵袭、细胞周期和细胞对舒尼替尼敏感性的影响。 方法构建针对p38的siRNA531和siRNA659两条siRNA，分别将其转染至肾癌786-O细胞株，即为siRNA531组和siRNA659组，同时设置转染无义siRNA的阴性对照组和仅加转染试剂的空白对照组。应用RT-PCR技术检测786-O细胞p38 mRNA的表达，蛋白质印迹法检测p38蛋白的表达。CCK-8法检测细胞的增殖情况和对舒尼替尼的敏感性，流式细胞术检测细胞的周期改变情况，Transwell实验检测细胞的侵袭能力。 结果RT-PCR及蛋白质印迹法检测发现siRNA转染后786-O细胞p38 mRNA及蛋白的表达均降低。与空白对照组和阴性对照组相比，siRNA531组和siRNA659组786-O细胞在转染后3~5 d时的增殖率均降低（P < 0.05，P < 0.01），细胞对舒尼替尼的敏感性增加，两组对舒尼替尼的IC50值均低于阴性对照组[（3.2±0.3）、（1.4±0.1）μmol/mL vs（5.4±0.2）μmol/mL，P < 0.05]。siRNA531组、siRNA659组G1期细胞数量明显多于对照组，且两组786-O细胞出现G0/G1阻滞。转染24 h后，两组的穿膜细胞数分别为56.43±6.02、34.00±8.12，与阴性对照组（76.27±5.08）相比，两组细胞的侵袭能力均下降（P < 0. 01）。 结论通过转染p38特异性siRNA可以成功沉默肾癌细胞株786-O的p38基因的表达，抑制肾癌细胞株786-O的增殖、侵袭能力，增加其对舒尼替尼的敏感性，为后续研究肾癌治疗及靶向耐药奠定基础。