Waqas Wakil

Multiple Resistances Against Formulated Organophosphates, Pyrethroids, and Newer-Chemistry Insecticides in Populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Pakistan

ABSTRACT Field populations of Helicoverpa armigera Hübner from 15 localities across the Punjab, Pakistan, were assessed by the leaf dip method for resistance against formulated organophosphates, pyrethroids, and newer insecticide groups. Resistance levels in H. armigera have been incrementally increasing for organophosphate and pyrethroid insecticides after decades of use in Pakistan. Resistance ratios (RRs) documented for organophosphates were 24- to 116-fold for profenofos and 22- to 87-fold for chlorpyrifos. For pyrethroids, RRs were 3- to 69-fold for Cypermethrin and 3- to 27-fold for deltamethrin. Resistance levels against newer chemistries were 2- to 24-fold for chlorfenapyr, 1- to 22-fold for spinosad, 1- to 20-fold for indoxacarb, 1- to 18-fold for abamectin, and 1- to 16-fold for emamectin benzoate. Resistant populations of H. armigera were mainly in the southern part of the Punjab, Pakistan. The most resistant populations were collected from Pakpattan, Multan, and Muzzafargarh. Of the nine insecticides tested, LC50 and LC90 values were lower for newer insecticide groups; resistance levels were moderate to very high against organophosphates, very low to high against pyrethroids, and very low to low against the newerchemistry insecticides. These findings suggest that the newer-chemistry insecticides with different modes of action could be included in insecticide rotations or replace the older insecticides. Supplementing the use of synthetic insecticides with safer alternatives could help to successfully lower the farmers reliance on insecticides and the incidence of resistance due to repeated use of insecticides against major insect pests.

Insecticidal Efficacy of Azadirachta indica, Nucleopolyhedrovirus and Chlorantraniliprole Singly or Combined against Field Populations fo Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)

Se determino la eficacia insecticida de formulaciones de Azadirachta indica, Nucleopolihedrovirus (VPN) y el nuevo insecticida diamida antranilico (clorantraniliprol) en contra de segundo, tercero, cuarto y quinto estadios larvales de Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) recogidos de diversas ubicaciones geograficas de la provincia de Punjab, Pakistan. Azadirachta indica se aplico en dosis de 5 μL L-1; VPN en dosis 2.1 x 105 POB mL-1 y clorantraniliprol fue 0,01 μL L-1 ya sea solos o en combinaciones. Los bioensayos se realizaron a 27 ± 1 °C y 65 ± 5% de humedad relativa. La mortalidad fue notablemente variada entre los tratamientos, estadios larvales y diversas poblaciones. Las combinaciones de VPN con A. indica y clorantraniliprol dio una mayor mortalidad, la fase de pupa y el efecto aditivo producido en comparacion con su aplicacion solo en todas las poblaciones de la prueba. La poblacion de Rawalpindi fue siempre susceptible, mientras que Gujranwala fue resistente. Los resultados del presente trabajo sugieren que la eficacia de VPN y A. indica pueden ser beneficiados por la presencia de clorantraniliprol contra las larvas de H. armigera.

Aptness of Beauveria bassiana and enhanced diatomaceous earth (DEBBM) for control of Rhyzopertha dominica F.

A diatomaceous earth formulation enhanced with bitterbarkomycin (DEBBM) combined with Beauveria bassiana (Balsamo) Vuillemin was evaluated against lesser grain borer Rhyzopertha dominica F. (Coleoptera: Bostrychidae) under laboratory conditions. DEBBM was applied at the rates of 15 and 30 ppm alone as well as in combination with 6.69 × 106, 6.69 × 108 and 6.69 × 1010 conidia/kg of wheat. Mortality of treated adults was recorded after 5, 10 and 15 days of exposure. Bioassays were carried out at 20, 25 and 30°C with 55 and 75% relative humidity. The emergence of progeny was also assessed 60 days post exposure. The combined use of DEBBM and B. bassiana considerably increased adult mortality especially at increasing temperatures and longer exposure intervals compared with DEBBM and B. bassiana alone. Progeny production was less in wheat treated with high dose rates of DEBBM +B. bassiana. The per cent mycosis in the cadavers was maximum where B. bassiana was applied at low dose rates. The results of the present study indicated that a combination of DEBBM and B. bassiana may provide effective control of R. dominica.

Integrated management of Helicoverpa armigera in chickpea in rainfed areas of Punjab, Pakistan

The pod borer Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) is an important insect pest of chickpea and several other crops in Asia, Africa and Australia. The present studies were carried out on integration of various practices for the management of H. armigera in Punjab, Pakistan, during 2004-05. Chickpea variety ‘CM-2000’ was selected for evaluating various strategies such as cultural, mechanical, biological, microbial, and chemical control, individually and in various possible combinations, for the management of H. armigera at three different locations. Integration of weeding, hand picking and indoxacarb (an oxadiazine insecticide) proved to be the most effective in reducing the larval population and pod infestation and resulted in the maximum grain yield. The larval population of the combined effect of these practices was not significantly different from hand picking + indoxacarb, weeding + indoxacarb, and indoxacarb alone. Hand picking in combination with Bacillus thuringiensis, and weeding + hand picking, also controlled the larval population significantly, but was inferior to the above treatments. The release of Trichogramma chilonis Ishii (Hymenoptera: Trichogrammatidae) did not control H. armigera. It was concluded on the basis of the cost — benefit ratio that hand picking was the most cost-effective method for the control of H. armigera, while the maximum increase in grain yield was obtained in plots with weeding, hand picking, and indoxacarb.

Trends in integrated pest management strategies for the control of Helicoverpa armigera (Hübner) caterpillars on chickpea (Cicer arietinum L.).

A study was carried out at the University of Arid Agriculture, Rawalpindi, Pakistan to integrate different control measures against Helicoverpa armigera (Hübner) on chickpea, to minimize the use of hazardous insecticides and develop an eco‐friendly management program. Cultural (weeding), mechanical (hand picking), biological (release of Trichogramma chilonis), microbial (spraying of Bacillus thruingiensis) and chemical (Steward) control practices were applied three times at 10 day intervals individually and also in various combinations. The application of Steward proved most effective when applied alone, with 0.41 larvae/plant, pod infestation of 9.31% and the highest grain yield (1203.66 g/plot); however, the integration of weeding, hand picking and Steward proved to be the most effective in reducing the larval population (0.12 larvae/plant) with minimum pod infestation (5.45%) on variety CM‐2000, which resulted in the maximum grain yield (1260.33 g/plot). The cost–benefit ratio in the treatment where Steward was applied alone was 1:2.20, and it was 1:3.53 where weeding and hand picking practices were integrated.

INDUCTION OF RESISTANCE IN CHICKPEA (Cicer arietinum L.) AGAINST Ascochyta rabiei BY APPLYING CHEMICALS AND PLANT EXTRACTS

Since new fungicides are scarce in the market and because of environmental problems, researchers are now emphasizing other alternatives, such as the genetic potential of plants, resistance against pathogens, and the use of biotic and abiotic agents to develop induced or acquired resistance. We investigated the role of resistance-inducing substances (chemicals and plant extracts) in three chickpea cvs. C-44, Pb-91, and Bittle-98 in field experiments against Ascochyta blight disease. These cultivars were selected on the basis of better yield potential shown in previous experiments. Aqueous solution of salicylic acid (SA) at 0.5, 1.0, and 1.5 mM; acibenzolar-S-methyl (ASM) at 0.4, 0.8, and 1.2 mM; and KOH at 25, 50, and 75 mM were applied, whereas Azadirachta indica A. Juss., Datura metel L., and Allium sativum L. plant extracts were applied at 5, 10, and 15%. Disease reduction data was recorded at different intervals from 4 to 14 d after being induced and inoculated with the pathogen. Overall results revealed that significant disease reduction (79.3%) was provided by ASM in the cv. C-44 at 1.2 mM compared with SA, whereas the least significant was KOH. Maximum disease reduction (43.5%) against disease in the plant extracts was observed by applying A. indica leaf extract, but D. metel and A. sativum extracts were not effective. Our findings suggest that enhancing resistance before infecting chickpea plants could be an innovative control method for Ascochyta blight of chickpea.

Distribution of Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) in tomato fields and its relationship to weather factors

A survey was conducted in tomato fields at different localities to assess the distribution of tomato fruitworm Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) in Punjab Province, Pakistan. The effect of weather factors on its population was also investigated. Eleven tomato‐growing districts were selected and data for larval population and fruit infestation were recorded. The maximum larval population (5.2 larvae/plant) of H. armigera was recorded in Bahawalpur district with 32.6% fruit infestation. However, the minimum larval population (1.4 larvae/plant) inflicted 14.7% fruit infestation in Rawalpindi district. The temperature was positively correlated while relative humidity showed negative interaction with the larval population and fruit infestation.

Entomopathogenic fungus as a biological control agent against Rhyzopertha dominica F. (Coleoptera: Bostrychidae) on stored wheat

To evaluate the pathogenicity of Metarhizium anisopliae (Metschinkoff) Sorokin (Deuteromycotina: Hyphomycetes) a bioassay was designed under laboratory conditions against Rhyzopertha dominica F. (Coleoptera: Bostrychidae) on stored wheat. The fungus was applied at the dose rates of 8 × 103, 8 × 105, 8 × 107 and 8 × 109 conidia/kg of wheat and the bioassay was conducted at 25°C with 60% relative humidity. The data regarding the mortality was recorded after 7 and 14 days exposure intervals. All the treatments gave the significant mortality of R. dominica and M. anisopliae of 8 × 109 conidia/kg was found to be the most effective after a 14-day exposure interval. There was greater production of progeny when the low rate of M. anisopliae was applied to wheat. Overall, our study showed that M. anisopliae is vigorous when applied at a high dose rate which revealed an effective control of R. dominica and also played a pivotal role in the integrated pest management program (IPM) of stored wheat insect pests.

Effect of modified meridic diet on the development and growth of tomato fruitworm Helicoverpa armigera (Lepidoptera: Noctuidae)

The efficacy of one new modified and two old meridic diets on Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) for rearing six successive generations was studied. Duration of larval development for insects fed on the modified diet was considerably shortened as most of them went through only five stadia before pupation, while the per cent pupation and per cent eclosion were relatively higher than on other diets. The lowest pupal mortality (6.33 ± 0.13%) was recorded in the F1 generation reared on the modified diet, whereas the highest pupal mortality (19.49 ± 0.15%) was observed in insects reared on a natural diet in the F6 generation. Blending of chickpea Cicer arietinum L. and red kidney bean Phaseolus vulgaris L. flours with tomato paste proved highly favorable for adult reproduction. These results suggest that the vitality of the tomato fruitworm did not decline obviously after rearing on a modified diet for several generations.

Sustainable Pest Management in Date Palm: Current Status and Emerging Challenges

Date palm, Phoenix dactylifera , is a monocotyledonous species belonging to the palm family (Arecaceae or Palmae) which is perennial and dioecious and cultivated mostly in the arid regions of the world. Date palm is important to the agrarian economy of several countries, with the ability to withstand severe abiotic stresses prevalent in the world’s arid regions, including hot and dry climatic conditions, water stress and salinity. A recent report on the arthropod fauna of date palm lists 112 species of insects and mites associated with it worldwide, including 22 species attacking stored dates. In several date producing countries, the monoculture type of date palm cultivation, climate change, unrestrained use of chemical insecticides and extensive international trade is likely to impact the pest complex and its natural enemies in the date agroecosystems. Considering the signifi cance of date palm, we summarize the biology and sustainable management of major insect and mite pests addressing related challenges and future research areas. The emerging role of semiochemicals in date palm IPM is described including new strategies in W. Wakil (*) Department of Entomology , University of Agriculture , Faisalabad , Punjab , Pakistan e-mail: waqaswakeel@hotmail.com; arid1972@yahoo.com J. R. Faleiro Food and Agriculture Organization of the United Nations (UTF/SAU/043/SAU) , Date Palm Research Center , Al-Ahsa , Saudi Arabia e-mail: jrfaleiro@yahoo.co.in T. A. Miller Department of Entomology , University of California , Riverside , CA , USA e-mail: thomas.miller@ucr.edu G. O. Bedford Department of Biological Sciences , Macquarie University , NSW 2109 , Australia e-mail: geoffrey.bedford@mq.edu.au R. R. Krueger USDA-ARS, National Clonal Germplasm Repository for Citrus and Dates , Riverside , CA , USA e-mail: robert.krueger@ars.usda.gov