Warwick L. Marsden

Enzymatic Hydrolysis of Cellulose in Lignocellulosic Materials

The aim of this review is to examine the literature relevent to the hydrolysis of cellulose in lignocellulosic materials. It is a complex process involving the nature of the cellulose itself and its association with other substances such as lignin and hemicellulose, as well as a multicomponent enzyme system which appears to be able to hydrolyze the cellulose in at least two ways. Any process to convert this cellulose to monomeric glucose requires both pretreatment and hydrolysis steps. The two steps are interdependent and it is not practical to study them in isolation. 350 references.

Alternative pathway for glucose production from cellulose using Trichoderma reesei QM9414 cellulase

SummaryEvidence is presented which supports the view that two routes exist for the formation of glucose when cellulosic material is saccharified using T. reesei enzyme preparations. The first is via cellobiose and for the second, glucose appears to be formed by a route not involving cellobiose. The second route becomes more apparent when dealing with less crystalline cellulose. This should be considered when constructing strains to produce enzyme preparations for saccharification of less crystalline cellulose.

Action of Trichoderma reesei QM9414 and C30 cellulase on substrates with varying crystallinity

Abstract The action of cellulase [see 1,4-(1,3;1,4)-β- d -glucan 4-glucanohydrolase, EC 3.2.1.4] preparations from Trichoderma reesei QM9414 and C30 has been compared on Sigmacell, Solka Floc and alkali-treated bagasse in the presence and absence of added d -glucose and cellobiose. On the basis of equal filter paper activity the two preparations acted similarly on the two cellulosic substrates, while in the case of alkali-treated bagasse the C30 preparation gave greater d -glucose release. The relative levels of cellobiose produced from alkali-treated bagasse suggests that the non-cellobiose route was more important in d -glucose release by the C30 preparation compared to the QM9414 preparation.