Wei Junfu

Protein molecularly imprinted polysiloxane prepared using calcium silicate/calcium alginate composite membrane as the matrix

Calcium silicate/calcium alginate composite hydrogel membrane (CaSiO3/CaAlg) was prepared by cross-linking the mixture of calcium silicate and sodium alginate with CaCl2 solution. Then the Haemoglobin (Hb) molecularly imprinted polysiloxane (MIP) was synthesized using CaSiO3/CaAlg composite hydrogel membrane as the matrix to adsorb Hb (the template), using Ethylenetri(β-methoxy)ethyoxysilane (KH-570) and γ-amidopropyltriethyoxysilane (KH-550) as functional monomers. The surface morphology and the thermal performance of the MIP membrane were characterized by scanning electron microscope (SEM) and thermogravimetric (TG) analysis. The surface contact angle and the swelling properties of the MIPs prepared with different silanes were studied. The adsorption properties of MIP and non-imprinted polysiloxane (NIP) were evaluated and the results showed that MIP membrane exhibited a significant improvement in terms of rebinding capacity for Hb compared with NIP. The MIP membrane could selectively recognize the template protein. The curves of Hb adsorption for Both MIP and NIP membrane are more in line with the Langmuir adsorption model. The adsorption rate of the MIP prepared in this paper was fast, the adsorption quantity was big and the MIP membrane was easy to be used. The MIP membrane could be widely used in protein separation and analysis, protein control release and biomedical engineering.