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Dive into the research topics where Wen-Bo Deng is active.

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Featured researches published by Wen-Bo Deng.


Journal of Biological Chemistry | 2012

Progesterone and DNA Damage Encourage Uterine Cell Proliferation and Decidualization through Up-regulating Ribonucleotide Reductase 2 Expression during Early Pregnancy in Mice

Wei Lei; Xu Hui Feng; Wen-Bo Deng; Hua Ni; Zhi Rong Zhang; Bo Jia; Xin Ling Yang; Tong-Song Wang; Ji-Long Liu; Ren Wei Su; Xiao Huan Liang; Qian Rong Qi; Zeng-Ming Yang

Background: Ribonucleotide reductase M2 (RRM2) is a rate-limiting step for DNA synthesis. It is still unknown how RRM2 is involved in decidualization. Results: RRM2 is highly expressed in the decidua and up-regulated by progesterone and DNA damage. Decidualization is significantly inhibited by specific RRM2 inhibitors. Conclusion: RRM2 is essential for mouse decidualization. Significance: This study will shed light on understanding the mechanism underlying decidualization. Embryo implantation into the maternal uterus is a crucial step for the successful establishment of mammalian pregnancy. Following the attachment of embryo to the uterine luminal epithelium, uterine stromal cells undergo steroid hormone-dependent decidualization, which is characterized by stromal cell proliferation and differentiation. The mechanisms underlying steroid hormone-induced stromal cell proliferation and differentiation during decidualization are still poorly understood. Ribonucleotide reductase, consisting of two subunits (RRM1 and RRM2), is a rate-limiting enzyme in deoxynucleotide production for DNA synthesis and plays an important role in cell proliferation and tumorgenicity. Based on our microarray analysis, Rrm2 expression was significantly higher at implantation sites compared with interimplantation sites in mouse uterus. However, the expression, regulation, and function of RRM2 in mouse uterus during embryo implantation and decidualization are still unknown. Here we show that although both RRM1 and RRM2 expression are markedly induced in mouse uterine stromal cells undergoing decidualization, only RRM2 is regulated by progesterone, a key regulator of decidualization. Further studies showed that the induction of progesterone on RRM2 expression in stromal cells is mediated by the AKT/c-MYC pathway. RRM2 can also be induced by replication stress and DNA damage during decidualization through the ATR/ATM-CHK1-E2F1 pathway. The weight of implantation sites and deciduoma was effectively reduced by specific inhibitors for RRM2. The expression of decidual/trophoblast prolactin-related protein (Dtprp), a reliable marker for decidualization in mice, was significantly reduced in deciduoma and steroid-induced decidual cells after HU treatment. Therefore, RRM2 may be an important effector of progesterone signaling to induce cell proliferation and decidualization in mouse uterus.


Journal of Biological Chemistry | 2014

Egr1 Protein Acts Downstream of Estrogen-Leukemia Inhibitory Factor (LIF)-STAT3 Pathway and Plays a Role during Implantation through Targeting Wnt4

Xiao-Huan Liang; Wen-Bo Deng; Ming Li; Zhen-Ao Zhao; Tong-Song Wang; Xu-Hui Feng; Yu-Jing Cao; Enkui Duan; Zeng-Ming Yang

Background: The downstream molecules of estrogen-LIF-STAT3 pathway during implantation are still unclear. Results: Egr1 is regulated by estrogen through LIF-STAT3 pathway in mouse uterus and regulates decidualization by targeting Wnt4. Conclusion: We showed Egr1 as a downstream target of LIF-STAT3 pathway and its involvement in decidualization. Significance: Our data could be a valuable source for future study on embryo implantation. Embryo implantation is a highly synchronized process between an activated blastocyst and a receptive uterus. Successful implantation relies on the dynamic interplay of estrogen and progesterone, but the key mediators underlying embryo implantation are not fully understood. Here we show that transcription factor early growth response 1 (Egr1) is regulated by estrogen as a downstream target through leukemia inhibitory factor (LIF) signal transducer and activator of transcription 3 (STAT3) pathway in mouse uterus. Egr1 is localized in the subluminal stromal cells surrounding the implanting embryo on day 5 of pregnancy. Estrogen rapidly, markedly, and transiently enhances Egr1 expression in uterine stromal cells, which fails in estrogen receptor α knock-out mouse uteri. STAT3 is phosphorylated by LIF and subsequently recruited on Egr1 promoter to induce its expression. Our results of Egr1 expression under induced decidualization in vivo and in vitro show that Egr1 is rapidly induced after deciduogenic stimulus. Egr1 knockdown can inhibit in vitro decidualization of cultured uterine stromal cells. Chromatin immunoprecipitation data show that Egr1 is recruited to the promoter of wingless-related murine mammary tumor virus integration site 4 (Wnt4). Collectively, our study presents for the first time that estrogen regulates Egr1 expression through LIF-STAT3 signaling pathway in mouse uterus, and Egr1 functions as a critical mediator of stromal cell decidualization by regulating Wnt4.


Reproductive Sciences | 2013

The Mesenchymal–Epithelial Transition During In Vitro Decidualization

Xiu-Hong Zhang; Xuan Liang; Xiao-Huan Liang; Tong-Song Wang; Qian-Rong Qi; Wen-Bo Deng; Ai-Guo Sha; Zeng-Ming Yang

The epithelial–mesenchymal transition plays a critical role in embryonic development, cancer progression, and metastasis. Decidualization is the process by which the fibroblast-like endometrial stromal cells differentiate into polygonal epithelial-like cells. However, it is still unclear whether mesenchymal–epithelial transition (MET) occurs during decidualization. The aim of this study was to examine whether decidualization causes the downregulation of some mesenchymal markers and upregulation of some epithelial markers in cultured uterine stromal cells. We showed that decidualization causes the downregulation of snail and vimentin expression, and upregulation of E-cadherin and cytokeratin expression. During in vitro decidualization, cultured stromal cells lose elongated shape and show epithelium-like characteristics. Our data suggest that the process of MET may exist during decidualization.


Scientific Reports | 2016

Non-coding RNA LINC00473 mediates decidualization of human endometrial stromal cells in response to cAMP signaling

Xiao-Huan Liang; Wen-Bo Deng; Yue-Fang Liu; Yu-Xiang Liang; Zong-Min Fan; Xiao-Wei Gu; Ji-Long Liu; Aiguo Sha; Hong-Lu Diao; Zeng-Ming Yang

Decidualization is an essential step in the establishment of pregnancy. However, the functional contributions of long intergenic noncoding RNAs (LincRNAs) to decidualization have not been explored. To explore the regulation and role of LincRNAs during human decidualization, human endometrial stromal cells (HESCs) are induced to undergo in vitro decidualization by treating with estradiol-17β, db-cAMP and medroxyprogesterone acetate. LINC00473 (LINC473) expression is highly induced in HESCs after decidual stimulus. We found that cAMP-PKA pathway regulates the expression of LINC473 through IL-11-mediated STAT3 phosphorylation. RNA interference-mediated down-regulation of LINC473 inhibits in vitro decidualization. These results suggested that LINC473 might be functionally required for human decidualization. This is the first report demonstrating the presence of LincRNA during human decidualization.


Molecular and Cellular Endocrinology | 2013

Heparin-binding epidermal growth factor-like growth factor (HB-EGF) induction on Snail expression during mouse decidualization.

Xiu-Hong Zhang; Xuan Liang; Tong-Song Wang; Xiao-Huan Liang; Ru-Juan Zuo; Wen-Bo Deng; Zhi-Rong Zhang; Fu-Niu Qin; Zhen-Ao Zhao; Zeng-Ming Yang

Embryo implantation requires a precise synchronism between the receptive uterus and activated blastocyst and is regulated by complicated molecular networks. Although many implantation-related genes have been identified, the crosstalk among them is still unknown. Snail, a transcription repressor, plays a central role during epithelial-mesenchymal transition. Our previous study showed that Snail is highly expressed at implantation site in mouse uterus. This study was to examine how Snail is related with other implantation-related genes in mice. Uterine stromal cells were isolated from mouse uteri on day 4 of pregnancy and treated with HB-EGF. Snail was induced significantly by HB-EGF. By using specific inhibitors and siRNA, we demonstrated that HB-EGF induction on Snail expression is dependent on the EGFR-ERK-Stat3 pathway. Cox-2 was regulated by Snail. The current findings demonstrate that Snail can relate with HB-EGF, Stat3 and Cox-2 and may play a role during mouse embryo implantation and decidualization.


Endocrinology | 2014

Regulation and Function of Deiodinases During Decidualization in Female Mice

Wen-Bo Deng; Xiao-Huan Liang; Ji-Long Liu; Zeng-Ming Yang

Thyroid dysfunction during human pregnancy is closely related to serious pregnancy outcome. However, the regulation and function of thyroid hormones during early pregnancy are largely unknown. We found that type II deiodinase, an enzyme converting T4 to activated T3, is highly expressed in the mouse uterus on days 3 and 4 of pregnancy. Once the embryo implants into the receptive uterus, type III deiodinase (Dio3), a mainly paternally imprinted gene for inactivating T3, is significantly induced in the stromal cells and accompanied by DNA hypermethylation of intergenic differentially CpG methylation regions in the δ-like 1 homolog-Dio3 imprinting cluster. The concentration of uterine free T3 is actually decreased after embryo implantation. T3 induces Dio3 expression both in vivo and in vitro, suggesting a positive feedback loop. T3 addition or Dio3 knockdown compromises decidualization. These results indicate that the Dio3-mediated local T3 decrease is critical for decidualization of stromal cells during early pregnancy. Furthermore, we found that progesterone regulates Dio3 expression through its cognate receptor both in vivo and in vitro. Additionally, cAMP regulates Dio3 transcription through the protein kinase A-cAMP response element-binding protein pathway. The inhibition of the protein kinase A pathway results in decreased Dio3 expression and impaired decidualization. Dio3 opposite strand (Dio3os) expressed in a similar pattern to Dio3, is transcribed from the opposite strand of Dio3 and fine-tunes Dio3 expression during decidualization. Our data indicate that Dio3 is strongly expressed and tightly controlled during decidualization.


FEBS Letters | 2014

Differential expression and anti-oxidant function of glutathione peroxidase 3 in mouse uterus during decidualization

Xiu Xu; Jing-Yu Leng; Fei Gao; Zhen-Ao Zhao; Wen-Bo Deng; Xiao-Huan Liang; Yi-Juan Zhang; Zhi-Rong Zhang; Ming Li; Ai-Guo Sha; Zeng-Ming Yang

Glutathione peroxidase 3 (GPX3) is an important member of antioxidant enzymes for reducing reactive oxygen species and maintaining the oxygen balance. Gpx3 mRNA is strongly expressed in decidual cells from days 5 to 8 of pregnancy. After pregnant mice are treated with GPX inhibitor for 3 days, pregnancy rate is significantly reduced. Progesterone stimulates Gpx3 expression through PR/HIF1α in mouse endometrial stromal cells. In the decidua, the high level of GPX3 expression is closely associated with the reduction of hydrogen peroxide (H2O2). Based on our data, GPX3 may play a major role in reducing H2O2 during decidualization.


Fertility and Sterility | 2012

Arachidonic acid regulation of the cytosolic phospholipase A2α/cyclooxygenase-2 pathway in mouse endometrial stromal cells

Zhen-Ao Zhao; Zhi-Rong Zhang; Xiu Xu; Wen-Bo Deng; Ming Li; Jing-Yu Leng; Xiao-Huan Liang; Zeng-Ming Yang

OBJECTIVE To investigate the role of arachidonic acid (AA) in mouse endometrial stromal cells. DESIGN Experimental animal study. SETTING University research laboratory. ANIMAL(S) Sexually mature female CD1-strain mice. INTERVENTION(S) Primary culture of endometrial stromal cells. MAIN OUTCOME MEASURE(S) Western blot and real-time polymerase chain reaction for gene expression and/or phosphorylation analysis. Luciferase assay for Cox-2 promoter analysis. RESULT(S) AA-derived prostaglandins play important roles during embryo implantation and decidualization. However, the function of AA itself in reproduction is largely unknown. In this study, exogenous AA stimulated cPLA(2α) phosphorylation and COX-2 expression, mainly through ERK1/2 in mouse endometrial stromal cells, and p38 inhibitor modestly inhibited cPLA(2α) phosphorylation induced by AA. The induction of COX-2 by AA was diminished by short interfering RNA against C/EBPβ and inhibitory C/EBPβ (LIP). C/EBPβ binding site at -872--864 of Cox-2 promoter contributes to Cox-2 promoter activation induced by C/EBPβ transfection. The expression of C/EBPβ protein induced by AA was inhibited by p38 inhibitor, and the phosphorylation of C/EBPβ induced by AA was inhibited by p38 inhibitor and ERK1/2 inhibitor. A nonmetabolized analogue of AA (ETYA) also enhanced cPLA(2α) phosphorylation and COX-2 expression. The activation of cPLA(2α)/COX-2 by AA was not inhibited by COX inhibitor indomethacin. CONCLUSION(S) AA can induce cPLA(2α)/COX-2 pathway activation in mouse endometrial stromal cells.


FEBS Letters | 2016

Progesterone induces the expression of lipocalin‐2 through Akt‐c‐Myc pathway during mouse decidualization

Yue-Fang Liu; Wen-Bo Deng; Shu-Yun Li; Mei-Nan Yao; Jie Liu; Hai-Tin Dou; Meng-Long Zhao; Zeng-Ming Yang; Xiao-Huan Liang

Lipocalin‐2 (Lcn2) is a small glycoprotein involved in a number of biological processes such as inflammation and antibacterial response. In our study, Lcn2 is expressed in the subluminal stromal cells at implantation site on day 5 of pregnancy. The expression of Lcn2 in stromal cells is under the control of progesterone through Akt‐c‐Myc signaling pathway. Data from Lcn2 knockdown and recombinant protein treatments indicate that Lcn2 promotes mPGES‐1 expression in stromal cells. The expression of Lcn2 and mPGES‐1 is strongly stimulated by lipopolysaccharide (LPS), indicating that Lcn2 mediates LPS‐induced inflammation. These findings shed light on the role of Lcn2 during decidualization.


Molecular and Cellular Endocrinology | 2014

Cyclic adenosine monophosphate-induced argininosuccinate synthase 1 expression is essential during mouse decidualization

Zhu Huang; Tong-Song Wang; Yue-Chao Zhao; Ru-Juan Zuo; Wen-Bo Deng; Yu-Jing Chi; Zeng-Ming Yang

L-Arginine (L-Arg), a conditional essential amino acid in adults, has been shown to enhance pregnancy outcome. Argininosuccinate synthase (Ass1) and argininosuccinate lyase (Asl) are the key enzyme for L-Arginine (L-Arg) biosynthesis. Based our microarray analysis, Ass1 expression is upregulated significantly at implantation site on day 5 of pregnancy compared to that at inter-implantation site. However, the expression, regulation and function of Ass1 during early pregnancy remain unknown. Here we found that Ass1 is highly expressed in mouse decidua and uterine stromal cells undergoing decidualization, and Asl is weakly expressed in mouse decidua and uterine stromal cells undergoing decidualization. α-Methyl-DL-aspartic acid (MDLA), a specific inhibitor for Ass1, can significantly increase the rate of embryonic reabsorption. Under in vitro induced decidualization, MDLA clearly inhibits the expression of decidual/trophoblast prolactin-related protein (Dtprp), a marker for decidualization in mice. Only Ass1 expression is induced by cAMP through PKA/p-Creb signaling pathway. Results from our cell culture models further indicates that the high level of L-Arg enhances stromal proliferation, while enzymatic activity or Ass1 expression level is essential to determine the magnitude of both mouse and human decidualization. Interestingly, L-Arg at high concentration down-regulates Ass1 and Asl expression by negative feedback to maintain L-Arg homeostasis. These findings highlight that cAMP-induced Ass1 expression is important in controlling the magnitude of decidualization through regulating L-Arg level.

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Zeng-Ming Yang

South China Agricultural University

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Xiao-Huan Liang

South China Agricultural University

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Zhen-Ao Zhao

Chinese Academy of Sciences

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Ming Li

University of Maryland Center for Environmental Science

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Xiu Xu

Northeast Agricultural University

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Ji-Long Liu

South China Agricultural University

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