Wen-Yan Tian

CD40 signaling activated by agonistic anti-CD40 monoclonal antibody 5C11 has different effects on biological behavior of gastric carcinoma cells

A gastric cancer cell line, AGS has high-level expression of CD40. CD40 is present on the surfaces of a large variety of cells, including B cells, endothelial cells, dendritic cells and some carcinoma cells, and delivers signals regulating diverse cellular responses, such as proliferation, differentiation, growth suppression, cell death. In this research, the expression of CD40 and CD40 transcription in gastric cancer cell lines (AGS, BGC-823, HGC-27, SGC-7901) was investigated by semi-quantitative RT-PCR. Cancer cell proliferation inhibitory assay was also performed using different concentrations of anti-CD40 monoclonal antibody (mAb), 5C11. Results indicated that treatment of 5C11 alone and combined 5C11, IFN-alpha and 5-FU both significantly inhibited cancer cell proliferation. The synergistic effects of combined 5C11, IFN-alpha and 5-FU on growth inhibition of human gastric cancer AGS cells could be observed. These results suggest that humanized anti-CD40 monoclonal antibody (mAb), 5C11 can be used as a valuable reagent for clinical application.

Influence of sCD40L on gastric cancer cell lines

The CD40 signaling pathway plays a key role in tumor cell proliferation, differentiation, and apoptosis. Gastric cancer usually possesses a higher level of CD40 expression than normal tissue. We evaluated inhibition of soluble CD40 ligand (sCD40L) in apoptosis induction of gastric cancer cells. Gastric cancer cells (AGS and BGC-823) were incubated with sCD40L. Cell viability and cell cycle were determined by methyl-tetrazolium (MTT) assay and flow cytometry, respectively. The results showed that sCD40L hindered cell growth, arrested cells at G0/G1 phase and induced apoptosis. In conclusion, sCD40L suppress growth of gastric cancer cells through apoptosis induction and cell cycle quiescence. This work will provided a new approach to gene therapy of gastric cancer.

Analysis of the essential oil of magnolia and its effect on antioxidant enzyme activity, sCD40L and phosphorylate-activate Akt protein levels in gastric cancer mice

The chemical composition of essential oil from magnolia collected in Suzhou city, China, was studied using gas chromatography-mass spectroscopy. Fifty-four volatile compounds were identified in the essential oil, with eucalyptol (21.55%), β-Pinene(11.07%), D-Limonene (8.93%), 1R-α-Pinene (7.86%), Bicyclo[2.2.1]heptan-2-one, 1,7,7-trimethyl-, (1R)-(6.46%), Cyclohexene, 4-methylene-1-(1-methylethyl)- (5.30%), 1,4-Cyclohexadiene, 1-methyl-4-(1-methylethyl)- (5.10%), camphene (4.72%), being the major chemical constituents. In an in vivo approach, the protective effect of the essential oil against oxidative stress in gastric cancer (GC) mice was also investigated in terms of superoxide dismutase, CAT, and glutathione peroxidase activities. Our results indicate that the essential oil has an effective capability to enhance serum antioxidant enzyme activities and sCD40L, and decrease phosphorylate-activate Akt protein levels in a dose-dependent manner in gastric cencer mice. Overall, essential oil from magnolia appears to be an effective antioxidant and is quite suitable for further biological evaluation.