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Featured researches published by Wibowo Mangunwardoyo.


Malaria Journal | 2012

Existence of the rdl mutant alleles among the anopheles malaria vector in Indonesia

Puji Budi Setia Asih; Lepa Syahrani; Ismail E. Rozi; Nandha R Pratama; Sylvia S Marantina; Dian Sidik Arsyad; Wibowo Mangunwardoyo; William A. Hawley; Ferdinand Laihad; Shinta; Supratman Sukowati; Neil F. Lobo; Din Syafruddin

BackgroundThe gamma-aminobutyric acid (GABA) receptor-chloride channel complex is known to be the target site of dieldrin, a cyclodiene insecticide. GABA-receptors, with a naturally occurring amino acid substitution, A302S/G in the putative ion-channel lining region, confer resistance to cyclodiene insecticides that includes aldrin, chlordane, dieldrin, heptachlor, endrin and endosulphan.MethodsA total of 154 mosquito samples from 10 provinces of malaria-endemic areas across Indonesia (Aceh, North Sumatra, Bangka Belitung, Lampung, Central Java, East Nusa Tenggara, West Nusa Tenggara, West Sulawesi, Molucca and North Molucca) were obtained and identified by species, using morphological characteristic. The DNA was individually extracted using chelex-ion exchanger and the DNA obtained was used for analyses using sequencing method.ResultsMolecular analysis indicated 11% of the total 154 Anopheles samples examined, carried Rdl mutant alleles. All of the alleles were found in homozygous form. Rdl 302S allele was observed in Anopheles vagus (from Central Java, Lampung, and West Nusa Tenggara), Anopheles aconitus (from Central Java), Anopheles barbirostris (from Central Java and Lampung), Anopheles sundaicus (from North Sumatra and Lampung), Anopheles nigerrimus (from North Sumatra), whereas the 302 G allele was only found in Anopheles farauti from Molucca.ConclusionThe existence of the Rdl mutant allele indicates that, either insecticide pressure on the Anopheles population in these areas might still be ongoing (though not directly associated with the malaria control programme) or that the mutant form of the Rdl allele is relatively stable in the absence of insecticide. Nonetheless, the finding suggests that integrated pest management is warranted in malaria-endemic areas where insecticides are widely used for other purposes.


Journal of Microbiology | 2017

Antibacterial compound produced by Pseudomonas aeruginosa strain UICC B-40, an endophytic bacterium isolated from Neesia altissima

Rina Hidayati Pratiwi; Iman Hidayat; Muhammad Hanafi; Wibowo Mangunwardoyo

This study’s aim was to determine the identity of antibacterial compounds produced by Pseudomonas aeruginosa strain UICC B-40 and describe the antibacterial compounds’ mechanisms of action for damaging pathogenic bacteria cells. Isolation and identification of the compounds were carried out using thin layer chromatography (TLC), nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography mass spectrometry (LC-MS) analyses. Antibacterial activity was assayed via minimum inhibitory concentration (MIC) and the antibacterial compound mechanism was observed morphologically through scanning electron microscopy (SEM). This study successfully identified the (2E,5E)-phenyltetradeca-2,5-dienoate antibacterial compound (molecular weight 300 g/mol), composed of a phenolic ester, fatty acid and long chain of aliphatic group structures. MIC values for this compound were determined at 62.5 μg/ml against Staphylococcus aureus strain ATCC 25923. The mechanism of the compound involved breaking down the bacterial cell walls through the lysis process. The (2E,5E)-phenyltetradeca-2,5-dienoate compound exhibited inhibitory activity on the growth of Gram-positive bacteria.


INTERNATIONAL SYMPOSIUM ON CURRENT PROGRESS IN MATHEMATICS AND SCIENCES 2015 (ISCPMS 2015): Proceedings of the 1st International Symposium on Current Progress in Mathematics and Sciences | 2016

Fimbriae-C Salmonella typhi primers as clinical detection of typhoid disease by PCR methods

Muktiningsih Nurjayadi; Fera Kurniadewi; Irma Ratna Kartika; Suhartono; Restu Nidia Sandra; Fitri Wulandari; Taufan Ardianto; Dalia Sukmawati; Wibowo Mangunwardoyo

Salmonella typhi is bacteria causes typhoid disease in human. The mortality rate of typhoid disease in Indonesia is increasing. The most common detection method currently used is serological test. However, this method is often resulted in less accurate, less sensitive and specific detection. The previous research has successfully discovered fim-C-S. typhi gene which is able to code protein to contribute in adherent or colonization in human epithelial cell. The information of fim-C-S. typhi gene is used to develop more specific and more accurate detection method for S. typhi bacteria by amplification, characterization, specificity and sensitivity assay, and continue with clinical assay. The PCR result was the amplified 0.2 kilo base (kb) DNA fragment using the fimbriae-C-S. typhi primer pairs. The specificity assay was conducted by comparing the amplicon in size of 0.2 kb S. typhi with Gram negative bacteria genome with similar characteristics. The results indicated the fimbriae-C-S. typhi primers can diff...


Mycoscience | 2009

Lecanicillium and Verticillium species from Indonesia and Japan including three new species

Nampiah Sukarno; Yuko Kurihara; Muhammad Ilyas; Wibowo Mangunwardoyo; Erny Yuniarti; Wellyzar Sjamsuridzal; Ju-Young Park; Rasti Saraswati; Shigeki Inaba; Yantyati Widyastuti; Katsuhiko Ando; Shigeaki Harayama


Journal of Ethnopharmacology | 2015

The local knowledge of medicinal plants trader and diversity of medicinal plants in the Kabanjahe traditional market, North Sumatra, Indonesia.

Marina Silalahi; Nisyawati; Eko Baroto Walujo; Jatna Supriatna; Wibowo Mangunwardoyo


Mycoscience | 2008

Entomopathogenic fungi isolated from suspended-soil-inhabiting arthropods in East Kalimantan, Indonesia

Yuko Kurihara; Nampiah Sukarno; Muhammad Ilyas; Erny Yuniarti; Wibowo Mangunwardoyo; Rasti Saraswati; Ju-Young Park; Shigeki Inaba; Yantyati Widyastuti; Katsuhiko Ando


Mycoscience | 2008

Indonesian Kickxellales: two species of Coemansia and Linderina

Yuko Kurihara; Nampiah Sukarno; Muhammad Ilyas; Erny Yuniarti; Wibowo Mangunwardoyo; Ju-Young Park; Rasti Saraswati; Yantyati Widyastuti; Katsuhiko Ando


Makara Journal of Science | 2010

SELEKSI DAN PENGUJIAN AKTIVITAS ENZIM L-HISTIDINE DECARBOXYLASE DARI BAKTERI PEMBENTUK HISTAMIN

Wibowo Mangunwardoyo; Romauli Aya Sophia; Endang Sri Heruwati


Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan | 2010

Produksi Senyawa Bioaktif dari Aspergillus ustus MFW 26-08 yang Berasosiasi dengan Spons Laut dalam Berbagai Media

Asri Pratitis; Gintung Patantis; Wibowo Mangunwardoyo; Ekowati Chasanah


Jurnal Riset Akuakultur | 2016

UJI PATOGENISITAS DAN VIRULENSI Aeromonas hydrophila Stanier PADA IKAN NILA (Oreochromis niloticus Lin.) MELALUI POSTULAT KOCH

Wibowo Mangunwardoyo; Ratih Ismayasari; Etty Riani

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Achmad Dinoto

Indonesian Institute of Sciences

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Endang Sukara

Indonesian Institute of Sciences

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Nampiah Sukarno

Bogor Agricultural University

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Yantyati Widyastuti

Indonesian Institute of Sciences

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Andi Salamah

University of Indonesia

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Muhammad Ilyas

Indonesian Institute of Sciences

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Ju-Young Park

National Institute of Technology and Evaluation

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Katsuhiko Ando

National Institute of Technology and Evaluation

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Yuko Kurihara

National Institute of Technology and Evaluation

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