Wilfried Lubisch
Bosch
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Featured researches published by Wilfried Lubisch.
Biological Chemistry | 2004
Christiane Neuhof; Verena Fabiunke; Karin Deibele; Maria Speth; Achim Möller; Wilfried Lubisch; Hans Fritz; Harald Tillmanns; H. Neuhof
Abstract Two novel calpain inhibitors (A-705239 and A-705253) were studied in isolated perfused rabbit hearts subjected to 60-min occlusion of the ramus interventricularis of the left coronary artery (below the origin of the first diagonal branch), followed by 120 min of reperfusion. The inhibitors were added to the perfusion fluid in various final concentrations from the beginning of the experiments before the coronary artery was blocked. Hemodynamic monitoring and biochemical analysis of perfusion fluid from the coronary outflow were carried out. Myocardial infarct size and the area at risk (transiently non-perfused myocardium) were determined from left ventricular slices after a special staining procedure with Evans blue and 2,3,5-triphenyltetrazolium chloride. The infarcted area (dead myocardium) was 77.9±2.3% of the area at risk in untreated controls (n=12). The infarct size was significantly reduced in the presence of both calpain inhibitors. The best effect was achieved with 10-8 M A-705253 (n=8), which reduced (p<0.001) the infarcted area to 49.3±3.9% of the area at risk, corresponding to an infarct reduction of 61.8%. No statistical difference was observed between the experimental groups in coronary perfusion, left ventricular pressure, and in the release of lactate dehydrogenase and creatine kinase from heart muscle.
Biological Chemistry | 2003
Christiane Neuhof; Oliver Götte; Sonata Trumbeckaite; Markus Attenberger; Nermin Kuzkaya; Frank N. Gellerich; Achim Möller; Wilfried Lubisch; Maria Speth; Harald Tillmanns; H. Neuhof
Abstract The effects of the novel calpain inhibitor A-705239 were studied in isolated perfused rabbit hearts subjected to 45 min of global ischemia, followed by 60 min of reperfusion. During 15 min of perfusion the inhibitor accumulated in myocardial tissue up to 16 times the concentration in the perfusate. Almost complete recovery and survival of heart function (90%) was seen with an inhibitor concentration of 10 8 M in the perfusion fluid when the compound was administered prior to ischemia. Left ventricular pressure amplitude and coronary flow showed significantly higher values during reperfusion in the presence of the inhibitor. A-705239 significantly reduced the release of creatine kinase, from 166±49 U/l in untreated hearts to 44±10 U/l, and diminished the release of lactate dehydrogenase from 118±20 U/l in untreated hearts to 63±4 U/l. Mitochondrial dysfunction following ischemia and reperfusion was markedly attenuated by the inhibitor. Thus, the state 3 respiration rate only decreased to 4.2 in contrast to 2.6 nmol O2/(min×mg s.w.) in untreated hearts, reflecting a reduced damage of oxidative phosphorylation. Furthermore, in the presence of the inhibitor the inner mitochondrial membranes became less permeable as indicated by a smaller leak respiration. The excellent properties of A-705239 should make this compound a valuable tool for further pharmacological studies.
Archive | 2000
Wilfried Lubisch; Michael Kock; Thomas Höger; Roland Grandel; Uta Holzenkamp; Sabine Schult; Reinhold Müller
Archive | 1999
Wilfried Lubisch; Michael Kock; Thomas Höger; Sabine Schult; Roland Grandel; Reinhold Müller
Journal of Medicinal Chemistry | 2003
Wilfried Lubisch; Edith Beckenbach; Sabina Bopp; Hans-Peter Hofmann; Arzu Kartal; Claudia Kästel; Tanja Lindner; Marion Metz-Garrecht; Jutta Reeb; Ferdinand Regner; Michael Vierling; Achim Möller
Archive | 1999
Wilfried Lubisch; Michael Kock; Thomas Höger
Archive | 2004
Wilfried Lubisch; Wilfried Hornberger; Thorsten Oost; Daryl R. Sauer; Liliane Unger; Wolfgang Wernet; Hervé Geneste
Archive | 2001
Wilfried Lubisch; Michael Kock; Thomas Hoeger; Roland Grandel; Reinhold Mueller; Sabine Schult
Archive | 2000
Wilfried Lubisch; Jens Sadowski; Michael Kock; Thomas Höger
Archive | 1999
Wilfried Lubisch; Michael Kock; Thomas Höger