William C. Hatch
Albert Einstein College of Medicine
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by William C. Hatch.
Brain Research | 1992
William D. Lyman; William C. Hatch; E. Pousada; G. Stephney; William K. Rashbaum; Karen M. Weidenheim
We have previously reported the establishment of organotypic cultures derived from human fetal brain tissue. Although these cultures permit the testing of multiple hypotheses about normal human neurodevelopment and neuropathologic conditions, they have the limitation of not being myelinated and therefore preclude the study of questions related to myelinogenesis and diseases of myelin. In the current communication, we describe recent developments that allow us to overcome this limitation and permit the establishment of a myelinated organotypic culture model. Sections of dorsal column dissected from the lumbar spinal cord of human fetuses ranging in age 21-23 weeks of gestation were placed in culture. The explants were maintained for up to 12 weeks during which time they were characterized and shown to express a number of CNS cell-type-specific markers including glial fibrillary acidic protein (astrocytes), nerve growth factor receptor and neurofilament protein (neurons), CD68 (microglia), and myelin basic protein, HNK-1 and galactocerebroside (oligodendrocytes). In addition, lectin histochemistry using Ricinus communis agglutinin-1 detected microglia and endothelial cells. Upon explantation, abundant myelin was seen by electron microscopy in the cultures. Although during the culture period there was degradation of myelin, there was also evidence of maintenance of intact myelin sheaths around small caliber axons and de novo myelin synthesis. This model system may permit the further use of human organotypic cultures to investigate issues related to neurodevelopment and to pathologic conditions including those relevant to dysmyelination and demyelination.
Pediatric Hematology and Oncology | 1992
Y. Burstein; William K. Rashbaum; William C. Hatch; T. A. Calvelli; M. Golodner; William D. Lyman
Human granulocyte-macrophage colony-stimulating factor (GM-CSF) has been described as a multi-lineage growth factor that induces in vitro colony formation of bone marrow erythroid burst-forming units (BFU-E), multipotential colony-forming units (CFU-GEMM), granulocyte-macrophage CFU (CFU-GM), granulocyte CFU (CFU-G), macrophage CFU (CFU-M), as well as eosinophil colony-forming units (CFU-Eo). Because of the preeminent role of the liver in fetal hematopoiesis, the effect of human recombinant GM-CSF (hrGM-CSF) on hematopoietic cells isolated from human fetal liver was tested in liquid cultures and in semisolid colony assays. hrGM-CSF induced a significant increase in the number of mature eosinophils in liquid culture and to a lesser extent in semisolid cultures when compared to untreated culture controls. The kinetics of this effect on eosinophils reached its peak on day 21 of culture. When GM-CSF and erythropoietin (Ep) were added simultaneously to the cultures, no significant change in the number of eosinophils compared to hrGM-CSF alone was observed. Ep or granulocyte colony-stimulating factor (G-CSF) did not show any CFU-Eo activity when added separately or simultaneously to both liquid and semisolid cultures. These results indicate that hrGM-CSF alone may be a potent stimulating factor for CFU-Eo obtained from human fetal liver and, in combination with other growth factors, control optimal development of human fetal eosinophils.
American Journal of Pathology | 1993
Sunhee C. Lee; William C. Hatch; Wei Liu; Yvonne Kress; William D. Lyman; Dennis W. Dickson
Research publications - Association for Research in Nervous and Mental Disease | 1994
Dennis W. Dickson; Sunhee C. Lee; William C. Hatch; L. A. Mattiace; Celia F. Brosnan; William D. Lyman
Developmental Brain Research | 1991
William D. Lyman; M. Tricoche; William C. Hatch; Yvonne Kress; F.-C. Chiu; William K. Rashbaum
Journal of Acquired Immune Deficiency Syndromes | 1992
Kathryn E. Tanaka; William C. Hatch; Yvonne Kress; Ruy Soeiro; Theresa Calvelli; William K. Rashbaum; Arye Rubinstein; William D. Lyman
Annals of the New York Academy of Sciences | 1993
Sunhee C. Lee; William C. Hatch; Wei Liu; Celia F. Brosnan; Dennis W. Dickson
Journal of Immunology | 1992
William C. Hatch; Kathryn E. Tanaka; Theresa Calvelli; William K. Rashbaum; Yvonne Kress; William D. Lyman
Annals of the New York Academy of Sciences | 1993
William D. Lyman; William C. Hatch; Jorge N. Larocca; William K. Rashbaum
Archive | 1993
Lee Simon; William C. Hatch; Wei-Kuang Liu; Yvonne Kress; William D. Lyman; Dennis W. Dickson